Chemical composition, antioxidant and antimicrobial activity of Thymus praecox supercritical extracts
2016
Authors:
Petrović, Nada V.Petrović, Slobodan S.
Džamić, Ana M.
Ćirić, Ana
Ristić, Mihailo S.
Milovanović, Stoja L.
Petrović, Slobodan D.
Document Type:
Article (Published version)
,
© 2016 Elsevier B.V.
Metadata
Show full item recordAbstract:
The chemical composition, antimicrobial and antioxidant activity of Thymus praecox extracts obtained by
fractional supercritical fluid extraction (SFE) with carbon dioxide were investigated. Properties of super critical extracts and extracts obtained by Soxhlet extraction were compared. The chemical composition
of extracts was determined using GC–FID and GC–MS analytical methods. The antimicrobial activity of
extracts was evaluated against 16 pathogenic microorganisms using microdilution method. Antioxidant
potential of extracts to neutralize DPPH radicals was also investigated. Investigated supercritical extracts
showed better antimicrobial activity than commercial antibiotics (streptomycin and ampicillin) and com mercial fungicides (bifonazol and ketoconazole) against all tested strains. For bacteria MIC and MBC were
38–200 g/ml and 75–300 g/ml, respectively, while for fungi MIC and MFC were 17–150 g/ml and
35–300 g/ml, respectively.
Keywords:
Thymus praecox; Supercritical fluid extraction; Antimicrobial activity; Thymol; DPPH radicalsSource:
The Journal of Supercritical Fluids, 2016, 110, 117-125Funding / projects:
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-MESTD-Basic Research (BR or ON)-173021)
DOI: 10.1016/j.supflu.2016.01.001
ISSN: 0896-8446