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Modulation of tumor necrosis factor-mediated cell death by fullerenes
dc.creator | Harhaji-Trajković, Ljubica | |
dc.creator | Isaković, Aleksandra | |
dc.creator | Vučićević, Ljubica | |
dc.creator | Janjetović, Kristina | |
dc.creator | Misirkić Marjanović, Maja | |
dc.creator | Marković, Zoran | |
dc.creator | Todorović-Marković, Biljana | |
dc.creator | Nikolić, Nadežda | |
dc.creator | Vranješ-Đurić, Sanja | |
dc.creator | Nikolić, Zoran | |
dc.creator | Trajković, Vladimir | |
dc.date.accessioned | 2024-08-13T08:54:08Z | |
dc.date.available | 2024-08-13T08:54:08Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 0724-8741 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6942 | |
dc.description.abstract | Purpose. The fullerene (C60/C70 mixture—C60/70) nanocrystalline suspension prepared by solvent exchange method using tetrahydrofyran (THF/nC60/70) and polyhydroxylated C60/70 [C60/70(OH)n] were compared for their ability to modulate cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNF). Materials and Methods. TNF-induced cytotoxicity was assessed in L929 fibrosarcoma cells by crystal violet assay. The type of cell death (apoptosis/necrosis), production of reactive oxygen species, mitochondrial depolarization and caspase activation were determined by flow cytometry using the appropriate reporter dyes. Results. THF/nC60/70 augmented, while C60/70(OH)n reduced the cytotoxicity of TNF. The numbers of cells undergoing apoptosis/necrosis, as well as of those displaying the activation of apoptosis-inducing enzymes of caspase family, were respectively increased or reduced by THF/nC60/70 or C60/70(OH)n. The antioxidant N-acetylcysteine and mitochondrial permeability transition inhibitor cyclosporin A each partly blocked the cytotoxic action of TNF, indicating the involvement of oxidative stress and mitochondrial dysfunction in the TNF cytotoxicity. Accordingly, THF/nC60/70 or C60/70(OH)n potentiated or suppressed, respectively, TNF-triggered oxidative stress and mitochondrial depolarization. Conclusion. The ability of different fullerene preparations to modulate TNF-induced oxidative stress and subsequent cell death suggests their potential value in the TNF-based cancer therapy or prevention of TNF-dependent tissue damage | sr |
dc.language.iso | en | sr |
dc.publisher | Academic Press | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/145073/RS// | sr |
dc.rights | restrictedAccess | sr |
dc.source | Pharmaceutical Research | sr |
dc.subject | apoptosis | sr |
dc.subject | fullerene | sr |
dc.subject | necrosis | sr |
dc.subject | oxidative stress | sr |
dc.subject | tumor necrosis factor | sr |
dc.title | Modulation of tumor necrosis factor-mediated cell death by fullerenes | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2007 Springer Science + Business Media, LLC | sr |
dc.citation.issue | 6 | |
dc.citation.volume | 25 | |
dc.identifier.doi | 10.1007/s11095-007-9486-y | |
dc.identifier.pmid | 17999162 | |
dc.identifier.scopus | 2-s2.0-43149126321 | |
dc.identifier.wos | 000255753900012 | |
dc.citation.spage | 1365 | |
dc.citation.epage | 1376 | |
dc.type.version | publishedVersion | sr |
dc.citation.rank | M21 |