Inactivation of nanocrystalline C60 cytotoxicity by γ-irradiation
2006
Authors:
Isaković, AleksandraMarković, Zoran
Nikolić, Nadežda
Todorović-Marković, Biljana
Vranješ-Đurić, Sanja
Harhaji-Trajković, Ljubica
Zogović, Nevena
Romčević, Nebojša
Vasiljević-Radović, Dana
Dramićanin, Miroslav
Trajković, Vladimir
Document Type:
Article (Published version)
,
© 2006 by Elsevier Ltd.
Metadata
Show full item recordAbstract:
We investigated the effect of gamma-irradiation on the cytotoxicity of pure C60 solubilized in water by using tetrahydrofuran (THF/n-C60 or THF/n-C60). In contrast to THF/n-C60, its gamma-irradiated counterpart failed to generate oxygen radicals and cause extracellular signal-regulated kinase (ERK)-dependent necrotic cell death in various types of mammalian cells. Moreover, gamma-irradiated THF/n-C60 protected cells from the oxidative stress induced by native THF/n-C60 or hydrogen peroxide. The observed biological effects were associated with gamma-irradiation-mediated decomposition of THF and subsequent derivatization of the n-C60 surface. These results for the first time demonstrate gamma-irradiation-mediated changes in the physico-chemical properties of THF-prepared nanocrystalline C60, resulting in a complete loss of its cytotoxic effect and its conversion to a cytoprotective agent.
Source:
Biomaterials, 2006, 27, 29, 5049-5058Funding / projects:
- Citotoksični, citoprotektivni i imunomodulatorni efekti nanočestica (RS-MESTD-MPN2006-2010-145073)
DOI: 10.1016/j.biomaterials.2006.05.047
ISSN: 0142-961
PubMed: 16784774