Acute-Phase-Dependent Binding Affinity of C/EBPβ from the Nuclear Extract and Nuclear Matrix towards the Hormone Response Element of the α2-Macroglobulin Gene in Rat Hepatocytes

Abstract

Interactions of nuclear extract and nuclear matrix proteins from rat hepatocytes with the hormone response element of the alpha2-macroglobulin gene were studied. By Western and South-Western blot analysis we have shown the presence of C/EBP beta in the examined nuclear fractions as well as its increased binding affinity to the examined gene sequence during the acute-phase response. The results suggest that both nuclear protein fractions could participate in the transcriptional regulation of the alpha2-macroglobulin gene in the rat hepatocytes.