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Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro
dc.creator | Brisevac, Dusica | |
dc.creator | Bajić, Aleksandar | |
dc.creator | Bjelobaba, Ivana | |
dc.creator | Milosević, Milena B | |
dc.creator | Stojiljković, Mirjana B | |
dc.creator | Beyer, Cordian | |
dc.creator | Clarner, Tim | |
dc.creator | Kipp, Markus | |
dc.creator | Nedeljković, Nadezda N | |
dc.date.accessioned | 2017-11-23T11:11:35Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2013 | sr |
dc.identifier.issn | 0895-8696 | sr |
dc.identifier.other | Rad_konverzija_2953 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/958 | |
dc.description.abstract | Nucleoside triphosphate diphosphohydrolases (NTPDases) are ecto-enzymes catalyzing the first step of sequential hydrolysis of extracellular ATP to adenosine, as the final product. Among eight members of NTPDase family, NTPDases1-3 have been shown to be expressed in the brain. Although altered NTPDase expression has been observed in relation to cell death and reactive gliosis in several experimentally induced neuropathologies, regulators of NTPDases expression and function are largely unknown. The present study explored the effects of several inflammatory factors (i.e., INF-gamma, TNF-alpha, LPS, peroxide, and glutamate) on NTPDase1-3 activity and expression by cultured cortical astrocytes. We were able to demonstrate that INF-gamma and TNF-alpha increased both ATP and ADP hydrolysis, while LPS specifically increased ATP hydrolysis. Consistent with the observed enhanced nucleotidase activity, INF-gamma induced the upregulation of NTPDase1 at the mRNA and protein level. Furthermore, we were able to demonstrate that INF-gamma and TNF-alpha decreased the relative abundance of dominant astrocytic NTPDase2 in favor of NTPDase1. In summary, these results suggest that INF-gamma, TNF-alpha, and LPS may be relevant in vivo regulators of NTPDase expression in neuropathologies associated with neuroinflammation. | en |
dc.description.sponsorship | Serbian Ministry of Education and Science [III41014]; DAAD/Serbian Ministry of Education and Science; DAAD in Germany; DAAD in Serbia; START grant of RWTH Aachen University, Medical Faculty | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Journal of Molecular Neuroscience | sr |
dc.title | Expression of Ecto-Nucleoside Triphosphate Diphosphohydrolase1-3 (NTPDase1-3) by Cortical Astrocytes After Exposure to Pro-inflammatory Factors In Vitro | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Беyер, Цордиан; Брисевац, Дусица; Бајић, Aлександар; Милосевић, Милена Б; Стојиљковић, Мирјана Б; Бјелобаба, Ивана; Цларнер, Тим; Кипп, Маркус; Недељковић, Надезда Н; | |
dc.citation.issue | 3 | sr |
dc.citation.volume | 51 | sr |
dc.citation.spage | 23 | sr |
dc.citation.epage | 879 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_958 |
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