Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis
2013
Аутори:
Saksida, TamaraMiljković, Đorđe
Timotijević, Gordana S
Stojanović, Ivana D.
Mijatović, Sanja
Fagone, Paolo
Mangano, Katia
Mammana, Santa
Farina, Claudio
Ascione, Ester
Maiello, Valentina
Nicoletti, Ferdinando
Stošić-Grujičić, Stanislava
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Transferrin (Tf) has a major role in T cell activation and proliferation. Here, we investigated whether Tf exerts immunomodulatory effects on T cells and in development of T-cell driven experimental autoimmune encephalomyelitis (EAE). While treatment of concanavalin A-stimulated splenocytes with apotransferrin (ApoTf) did not affect release of IL-1 beta, TNF, INF-gamma, IL-17, IL-4, and IL-10, it markedly and dose-dependently down-regulated synthesis of IL-2 in these cells. ApoTf also inhibited IL-2 generation in purified CD3(+) T cells and the effect was accompanied with down-regulation of MAPK p44/42 and NF kappa B signaling. Despite impeded IL-2 release, proliferation of splenocytes was not inhibited by ApoTf. Importantly, ApoTf ameliorated EAE in mice and significantly reduced ex vivo IL-2 production in proteolipid protein-specific lymphocytes. Thus ApoTf may be a promising beneficial agent for multiple sclerosis. (C) 2013 Elsevier B.V. All rights reserved.