Lemon extract reduces hepatic oxidative stress and persulfidation levels by upregulating Nrf2 and Trx1 expression in old rats
Running head: Lemon extract improves redox environment in old-aged livers

This document contains:

1. Table S1 - List of primer pairs used in qPCR analysis

Abbreviations
Gpx, glutathione peroxidase;
Gr, glutathione reductase;
Cat, catalase;  
Nrf2, nuclear factor erythroid 2-related factor 2; 
Sod1, superoxide dismutase 1; 
Sod2, superoxide dismutase 2; 
Trx1, thioredoxin1; 
Mst, 3-mercapto pyruvate sulfurtransferase; 
Cbs,  cystathionine beta-synthase; CSE,  cystathionine gamma-lyase;
Cse,  cystathionine gamma-lyase; 
Gst, glutathione S-transferase;  
Gclc, glutamate–cysteine ligase catalytic subunit; 
Gclm, glutamate–cysteine ligase modifier subunit;
Nqo1, NAD(P)H quinone dehydrogenase 1;
Sirt1, NAD-dependent deacetylase sirtuin-1;
Gapdh, Glyceraldehyde 3-phosphate dehydrogenase.


2. Table S2 - List of antibodies used in Western blot (WB), immunofluorescent (IF) analysis and persulfide detection (P-SSH)

Abbreviations
KEAP1, Kelch-like ECH-associated protein 1;
GPx, glutathione peroxidase;
GR, glutathione reductase;
CAT, catalase;  
NRF2, nuclear factor erythroid 2-related factor 2; 
SOD1, superoxide dismutase 1; 
SOD2, superoxide dismutase 2; 
TRX1, thioredoxin1; 
TRXR1, thioredoxin reductase 1; 
MST, 3-mercapto pyruvate sulfurtransferase; 
CBS,  cystathionine beta-synthase; CSE,  cystathionine gamma-lyase;
CSE,  cystathionine gamma-lyase;  
SIRT1, NAD-dependent deacetylase sirtuin-1;
GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.