Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase
2015
Autori:
Božić, IvaSavić, Danijela
Jovanović, Marija
Bjelobaba, Ivana
Laketa, Danijela
Nedeljković, Nadežda
Stojiljković, Mirjana
Peković, Sanja
Lavrnja, Irena
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Microglia play a key role in defending central nervous system from
various internal and external threats. However, their excessive and/or
chronic activation is associated with deleterious effects in a variety
of neurodegenerative diseases. Previously, we have shown that ribavirin
when applied in clinically relevant dosage (10 mu M) modulates activated
microglia in complex fashion inducing both anti-and proinflammatory
effects, simultaneously causing cytotoxicity. Here, we examined
potential of low-dose ribavirin (0.1 and 1 mu M) to modulate activated
BV-2 microglia. Morphological and functional activation of BV-2 cells
was achieved with lipopolysaccharide (LPS) stimulation. Our results
demonstrated that low-dose ribavirin did not induce cell death, while 10
mu M ribavirin promoted LPS induced apoptosis. We determined that 1 mu M
ribavirin was equally efficient in deactivation of LPS induced
morphological changes as 10 mu M ribavirin treatment. Ribavirin showed
halfway success in reducing markers of functional activation of
microglia. Namely, none of the doses had effect on LPS triggered
production of proinflammatory cytokine tumor necrosis factor alpha. On
the other hand, low-dose ribavirin proved its effectiveness in reduction
of another inflammatory mediator, nitric oxide, by inhibiting inducible
form of nitric oxide synthase. Our results imply that low-dose ribavirin
may alleviate nitrosative stress during neuroinflammation.
Izvor:
Analytical Cellular Pathology, 2015, 923614Finansiranje / projekti:
- Ćelijska i molekulska osnova neuroinflamacije: potencijala ciljna mesta za translacionu medicinu i terapiju (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
DOI: 10.1155/2015/923614
ISSN: 2210-7185