Correlation between Sperm Parameters and Protein Expression of Antioxidative Defense Enzymes in Seminal Plasma: A Pilot Study
2015
Аутори:
Macanović, BiljanaVučetić, Milica
Janković, Aleksandra
Stančić, Ana
Buzadžić, Biljana J.
Garalejić, Eliana
Korać, Aleksandra
Korać, Bato
Otašević, Vesna
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Background. Semen analysis is the cornerstone in the evaluation of male
(in) fertility. However, there are men with normal semen tests but with
impaired fertilizing ability, as well as fertile men with poor sperm
characteristics. Thus, there is rising interest to find novel parameters
that will help to predict and define the functional capacity of
spermatozoa. Methods. We examined whether there is a correlation between
semen parameters (count, progressive motility, and morphology) and
protein expression/activity of antioxidative defense enzymes in seminal
plasma from 10 normospermic subjects. Results. Sperm progressive
motility was in positive correlation with seminal plasma protein
expression of both superoxide dismutase (SOD) isoforms (MnSOD and
CuZnSOD) and catalase. Also, positive correlation was observed between
sperm count and MnSOD protein expression, as well as between sperm
morphology and protein expression of catalase in seminal plasma. In
contrast, protein expression of glutathione peroxidase was not in
correlation with any sperm parameter, while its activity negatively
correlated with sperm morphology and motility. Conclusions. These data
suggest that evaluation of protein expression of antioxidative defense
enzymes in seminal plasma might be of importance in the evaluation of
male fertility status and that could be used as an additional biomarker
along with classic semen analysis in assessment of semen quality.
Извор:
Disease Markers, 2015, 436236Финансирање / пројекти:
- Улога реактивних врста кисеоника и азота у репродукцији: могућа примена у лечењу хуманог стерилитета (RS-MESTD-Basic Research (BR or ON)-173054)
- COST BM1203
DOI: 10.1155/2015/436236
ISSN: 1875-8630