Modified Electrophysiological Responses of Neurons and Glial Cells Acutely Isolated From The Hibernating Land Snail
2014
Аутори:
Nikolić, LjiljanaBataveljić, Danijela
Moldovan, Ivana
Balind, Snežana
Todorović, Dajana
Nedeljković, Miodrag
Petković, Branka
Остала ауторства
Đurić, DraganJakovljević, Vladimir
Тип документа:
Конференцијски прилог (Објављена верзија)
,
© 2014 by the Serbian Physiological Society
Метаподаци
Приказ свих података о документуАпстракт:
Hibernation is a physiological state that enables certain animal species to survive severe
environmental conditions during the cold periods. In hibernation state the brain activity
is maintained at a very low, but functionally responsive level, indicating that neurons
and glial cells undergo certain physiological modifications. These modifications should
be characterized as a neuroprotective, since the brain of hibernated animals endures extreme
physiological conditions without any damage. In the present study we examined
the electrophysiological responses of neurons and glial cells all acutely isolated from
active and hibernating land snail Helix pomatia by the patch-clamp technique. Hibernating
state lasted six months. Data obtained revealed that the steady-state outward neuronal
current density was significantly lower in hibernation. In addition, the fast Na+
inward current density was significantly reduced in the population of isolated hibernated
neurons, indicating that neuronal activity is suppressed in hibernation. The lower neuronal
activity was supported by the suppressed electrophysiological response of glial
cells. Thus, hibernated glia had significantly lower specific membrane conductance and
reduced inward current density compared to active glia. Particularly important was the
modification of the glial inwardly rectifuing potassium (Kir) channel activity, essential
for the coupling of the function of glial cells with neuronal activity. Thus, in the presence
of BaCl2, Ba2+-sensitive current density mediated by the glial Kir channels was significantly
lower in hibernation. Altogether, our data indicate that overall suppression of
neuronal and glial activity is important natural neuroprotective strategy necessary.
Финансирање / пројекти:
- Утицај магнетних поља и других срединских стресора на физиолошке одговоре и понашање различитих врста (RS-MESTD-Basic Research (BR or ON)-173027)
У:
- Đurić D, Jakovljević V, editors. Molecular, Cellular and Integrative Basis of Health and Disease : transdisciplinary approach: abstract book. 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-31; Belgrade, Serbia. Belgrade: Serbian Physiological Society; 2014. p. 149.