C20-nor-Abietane and Three Abietane Diterpenoids from Plectranthus mutabilis Leaves as P-Glycoprotein Modulators.
2022
Authors:
Ntungwe, Epole N.Jovanović Stojanov, Sofija
Duarte, Noélia M.
Candeias, Nuno R.
Díaz-Lanza, Ana M.
Vágvölgyi, Máté
Hunyadi, Attila
Pešić, Milica
Rijo, Patrícia
Document Type:
Article (Published version)
,
© 2022 American Chemical Society
Metadata
Show full item recordAbstract:
In this study, a bioguided fractionation of Plectranthus mutabilis extract was performed by chromatographic methods. It yielded one new nor-abietane diterpene, mutabilol (1), and three known abietanes, coleon-U-quinone (2), 8α,9α-epoxycoleon-U-quinone (3), and coleon U (4). The abietane diterpenoid 5 was also tentatively identified using HPLC-MS/MS. Moreover, the extract profile and quantification of each isolated compound were determined by HPLC-DAD. Compound 4 was the major compound in the extract. Compounds 2-4 were found to be selective toward cancer cell lines and were able to inhibit P-glycoprotein (P-gp) activity in NCI-H460/R cells at longer exposure of 72 h and consequently revert doxorubicin (DOX) resistance in subsequent combined treatment. None of the compounds influenced the P-gp expression in NCI-H460/R cells, while the extract significantly increased it.
Keywords:
Abietane diterpenoids; Anticancer effect; Multidrug resistance; P-glycoprotein; Plectranthus mutabilisSource:
ACS Medicinal Chemistry Letters, 2022, 13, 4, 674-680Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.1021/acsmedchemlett.1c00711
ISSN: 1948-5875
PubMed: 35450348
WoS: 000791541600029
Scopus: 2-s2.0-85126589451
URI
https://pubs.acs.org/doi/10.1021/acsmedchemlett.1c00711http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9014510
http://radar.ibiss.bg.ac.rs/handle/123456789/4945