New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
2022
Аутори:
Smailagić, DijanaBanjac, Nevena
Ninković, Slavica
Savić, Jelena
Ćosić, Tatjana
Pěnčík, Aleš
Ćalić, Dušica
Bogdanović, Milica
Trajković, Milena
Stanišić, Mariana
Тип документа:
Чланак у часопису (Објављена верзија)
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© 2022 Smailagić, Banjac, Ninković, Savić, Ćosić, Pěnčík, Ćalić, Bogdanović, Trajković and Stanišić.
Метаподаци
Приказ свих података о документуАпстракт:
Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
Кључне речи:
allelopathy; ARD; auxin; dihydrochalcones; Malus×domestica Borkh. (apple); phloretin; phytotoxicity; polar auxin transportИзвор:
Frontiers in Plant Science, 2022, 13, 875528-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
DOI: 0.3389/fpls.2022.875528
ISSN: 1664-462X
PubMed: 35873993