Role of Sodium Nitroprusside on Potential Mitigation of Salt Stress in Centaury (Centaurium erythraea Rafn) Shoots Grown In Vitro
2023
Аутори:
Trifunović-Momčilov, MilanaStamenković, Nikola
Đurić, Marija
Milošević, Snežana
Marković, Marija
Giba, Zlatko
Subotić, Angelina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Soil salinity is one of the most common abiotic stressors that affects plant growth and
development. The aim of this work was to investigate the influence of sodium nitroprusside (SNP),
a donor of nitric oxide (NO), on the physiological response of common centaury (Centaurium erythraea)
shoots grown under stress conditions caused by sodium chloride (NaCl) in vitro. Centaury
shoots were first grown on nutrient medium containing different SNP concentrations (50, 100 and
250 μM) during the pretreatment phase. After three weeks, the shoots were transferred to nutrient
media supplemented with NaCl (150 mM) and/or SNP (50, 100 or 250 μM) for one week. The results
showed that salinity decreased photosynthetic pigments, total phenolic content and DPPH (1,1-diphenyl-
2-picrylhydrazyl radical) concentration. The activities of antioxidant enzymes, namely superoxide
dismutase (SOD), catalase (CAT) and peroxidase (POX), were also reduced under salt
stress. However, MDA concentration was decreased, while H2O2 and proline content did not drastically
change under the stress conditions caused by NaCl. Exogenous application of SNP altered
the biochemical parameters of centaury shoots grown under salt stress. In this case, increased photosynthetic
pigment content, total phenolics and proline content were noted, with reduced MDA,
but not H2O2, concentration was observed. In addition, the exogenous application of SNP increased
the degree of DPPH reduction as well as SOD, CAT and POX activities.
Кључне речи:
common centaury; salinity stress; Oxidative stress; antioxidative protection; sodium nitroprussideИзвор:
Life, 2023, 13, 1, 154-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
DOI: 10.3390/life13010154
ISSN: 2075-1729
PubMed: 36676103