Protective Effects of Arbutus unedo L. Honey in the Alleviation of Irinotecan-Induced Cytogenetic Damage in Human Lymphocytes-An In Vitro Study
2023
Autori:
Jurič, AndrejaBrčić Karačonji, Irena
Gašić, Uroš
Milojković Opsenica, Dušanka
Prđun, Saša
Bubalo, Dragan
Lušić, Dražen
Vahčić, Nada
Kopjar, Nevenka
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Strawberry tree (Arbutus unedo L.) honey (STH) has been used since ancient times as a folk medicine remedy, especially in certain Mediterranean countries. This honey, rich in phenolic content, is well recognized for its antioxidant, anti-inflammatory, and antimicrobial activities, and is used for the treatment of skin lesions as well as gastrointestinal and respiratory disorders. This study investigated whether STH alleviates genome damage in human peripheral blood lymphocytes produced by the cytotoxic drug irinotecan. The phenolic profile of STH was previously estimated by ultra-high-performance liquid chromatography coupled to a linear ion trap-Orbitrap hybrid mass spectrometer. The effects of STH were evaluated at three concentrations (1×, 5×, and 10×), based on the daily consumption of the honey by an adult person. After 2 h of in vitro exposure, standard lymphocyte cultures for the analysis of chromosome aberrations and the cytokinesis-block micronucleus cytome assay were established. Our results demonstrate that STH offered remarkable geno- and cytoprotection when administered with irinotecan. These findings are relevant for drawing preliminary conclusions regarding the in vitro safety of the tested honey. However, further studies are needed with the application of more complex experimental models.
Ključne reči:
Arbutus unedo; UHPLC-LTQ Orbitrap MS; chromosome aberration analysis; cytokinesis-block micronucleus cytome assay; honey; irinotecan; lymphocytes; phenolicsIzvor:
International Journal of Molecular Sciences, 2023, 24, 3, 1903-Finansiranje / projekti:
- Croatian Academy of Sciences and Arts
- Ministry of Science and Education of the Republic of Croatia
DOI: 10.3390/ijms24031903
ISSN: 1661-6596
PubMed: 36768223
Scopus: 2-s2.0-85147892926
URI
https://www.mdpi.com/1422-0067/24/3/1903http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9916164
http://radar.ibiss.bg.ac.rs/handle/123456789/5483