Membrane currents of C6 cells in pharmacological investigation
2022
Authors:
Nikolić, LjiljanaSavić, Danijela
Korać Jačić, Jelena
Petković, Branka
Stojadinović, Gordana
Martać, Ljiljana
Bogdanović Pristov, Jelena
Contributors
Čupić, ŽeljkoAnić, Slobodan
Document Type:
Conference object (Published version)
,
© 2022 by the Society of Physical Chemists of Serbia
Metadata
Show full item recordAbstract:
Living cells exhibit multiple ion channel proteins and malfunction of these channels underlies
numerous diseases. Channelopathies include diseases of the nervous, cardiovascular, respiratory,
endocrine, urinary, and immune system. Currently, ion channels represent the second-largest target
for existing drugs. Here, using the whole-cell patch-clamp technique, we explored the epinephrine
effect on membrane ionic currents in glioma C6 cells. We demonstrate that epinephrine specifically
evokes an increase of C6 cells outward ionic currents that is stable within 10 min, while it does not
affect inward currents. Our results thus provide fine resolution and time frame for targeting ion
channel activity that is crucial in pharmacological investigations.
Keywords:
glioma cells; pharmacology; patch-clampFunding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
In:
- Čupić Ž, Anić S, editors. Proceedings: Physical Chemistry 2022, Vol. 2. 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia. Belgrade: Society of Physical Chemists of Serbia; 2022. p. 589-92.