Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae
2023
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Temperature and food quality are the most important environmental factors determining
the performance of herbivorous insects. The objective of our study was to evaluate the responses of
the spongy moth (formerly known as the gypsy moth) [Lymantria dispar L. (Lepidoptera: Erebidae)] to
simultaneous variation in these two factors. From hatching to the fourth instar, larvae were exposed
to three temperatures (19 C, 23 C, and 28 C) and fed four artificial diets that differed in protein (P)
and carbohydrate (C) content. Within each temperature regime, the effects of the nutrient content
(P+C) and ratio (P:C) on development duration, larval mass, growth rate, and activities of digestive
proteases, carbohydrases, and lipase were examined. It was found that temperature and food quality
had a significant effect on the fitness-related traits and digestive physiology of the larvae. The greatest
mass and highest growth rate were obtained at 28 C on a high-protein low-carbohydrate diet. A
homeostatic increase in activity was observed for total protease, trypsin, and amylase in response to
low substrate levels in the diet. A significant modulation of overall enzyme activities in response
to 28 C was detected only with a low diet quality. A decrease in the nutrient content and P:C ratio
only affected the coordination of enzyme activities at 28 C, as indicated by the significantly altered
correlation matrices. Multiple linear regression analysis showed that variation in fitness traits in
response to different rearing conditions could be explained by variation in digestion. Our results
contribute to the understanding of the role of digestive enzymes in post-ingestive nutrient balancing
Кључне речи:
Lymantria dispar; temperature; dietary proteins and carbohydrates; development duration; larval mass; digestive enzymes; gypsy mothИзвор:
Biomolecules, 2023, 13, 5, 821-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)