Evaluation of the DNA Methylation Status of Procalcitonin Gene as a Biomarker of Local and Systemic Inflammation
2018
Аутори:
Rajić, JovanaGrdović, Nevena
Petrović Matić, Sanja
Dinić, Svetlana
Uskoković, Aleksandra
Mihailović, Mirjana
Arambašić Jovanović, Jelena
Tolić, Anja
Đorđević, Marija
Đorđević, Miloš
Poznanović, Goran
Pucar, Ana
Vidaković, Melita
Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2018 Silverchair Publisher
Метаподаци
Приказ свих података о документуАпстракт:
Objectives: Procalcitonin (PCT) has recently emerged as an important biomarker for an early and accurate diagnosis of bacterial infection, hence suggested as biomarker of periodontal disease caused by oral pathogenic microorganisms. Altered DNA methylation of PCT coding gene - calcitonin-related polypeptide a (CALCA) has been shown during systemic inflammation. The aim of this study was to evaluate the influence oflocal and/or systemic inflammation present during periodontitis and diabetes on DNA methylation status of CALCA and its potential use as epigeneticbased biomarker of these chronic inflammatory conditions.
Methods: The study included 65 individuals divided in three groups: healthy control (n = 17), periodontitis (n = 27) and diabetes/periodontitis group (n = 21). Periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999) while type 2 diabetes assessment was performed according to WHO criteria (2013). DNA methylation profile of CALCA promoter in buccal epithelial cells was analyzed by methylation specific polymerase chain reaction (MSP).
Results: Decrease in DNA methylation of CALCA promoter was observed in periodontitis and even more pronounced in diabetes/periodontitis compared to control group, although without statistical significance. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CALCA promoter and glycosylated hemoglobin. Even though it is known that life style affects DNA methylation patterns, there was no difference in DNA methylation of CALCA promoter between smokers/non-smokers and normal/overweight individuals.
Conclusions: Presented results suggest that local periodontal inflammation contributes to the change, but that only systemic inflammation significantly alters the DNA methylation profile of CALCA in buccal cells. Those results imply that DNA methylation status of CALCA reflects systemic inflammation, but additional studies are needed to estimate usefulness of this epigenetic-based biomarker for periodontal disease.
Финансирање / пројекти:
- European Cooperation in Science and Technology (COST) Action CA16112
- European Cooperation in Science and Technology (COST) CM1406
- European Cooperation in Science and Technology (COST) CA16119
- Сигнални молекули у дијабетесу: идентификација потенцијалних биолошких маркера укључених у модификацију и интеграцију сигналних путева у циљу предикције и интервенције у дијабетесу (RS-MESTD-Basic Research (BR or ON)-173020)
У:
- Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, Italy. Karger Publishers; 2018. p. 12. (Lifestyle Genomics; Vol. 11; No. 1).