Reduced and oxidized glutathione affects smooth muscle contractility and anti-oxidant enzymes activity in isolated ileum of male rat
2023
Аутори:
Grahovac, TanjaVidonja Uzelac, Teodora
Nikolić-Kokić, Aleksandra
Tatalović, Nikola
Oreščanin-Dušić, Zorana
Blagojević, Duško
Остала ауторства
Bajrović, KasimТип документа:
Конференцијски прилог (Објављена верзија)
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© 2023 by the Institute for Genetic Engineering and Biotechnology, University of Sarajevo
Метаподаци
Приказ свих података о документуАпстракт:
Normal redox status of the cell is maintained by a number of enzymes, such as superoxide dismutase, catalase, glutathione peroxidase and low molecular weight antioxidants. One of the component that determining the redox status of cells is the tripeptide glutathione. Maintaining high levels of reduced glutathione enhances antioxidant defense. The aim of this study was to examine whether, to what extent and in what way changes in physiological processes occurred in conditions when cellular redox homeostasis is in imbalance. The experiments were performed on isolated ileum of male Wistar rats, electrically stimulated and treated with cumulative doses of reduced or oxidized glutathione. The activity of antioxidant enzymes (catalase, glutathione reductase, glutathione peroxidase, copper–zinc superoxide dismutase and manganese superoxide dismutase) was determined in treated ileum. Glutathione in both forms decreased amplitude of ileum contractions. Cumulative doses of oxidized glutathione led to higher glutathione reductase activity while the addition of reduced glutathione increased activity of glutathione peroxidase. The results showed that maintenance of cellular redox homeostasis have influence to physiological processes.
Кључне речи:
redox homeostasis; oxidative stress; antioxidant enzymes; ileum; ratФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Bajrović K, editor. Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina -ABMBBIH; 2023 May 18-20; Sarajevo, Bosnia and Herzegovina. Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo; 2023. p. 42. (Genetics and Applications; Special edition).