Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis
2016
Аутори:
Stanisavljević, SuzanaLukić, Jovanka
Momčilović, Miljana
Miljković, Marija
Jevtić, Bojan
Kojić, Milan
Golić, Nataša
Mostarica Stojković, Marija
Miljković, Đorđe
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2016 Wageningen Academic Publishers
Метаподаци
Приказ свих података о документуАпстракт:
Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-γ and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.
Кључне речи:
DGGE; animal models; cytokine; faeces; gut-associated lymphoid tissues; immune systemИзвор:
Beneficial Microbes, 2016, 7, 3, 363-373Финансирање / пројекти:
- Ћелијски и молекулски механизми опоравка пацова од експерименталног аутоимунског енцефаломијелитиса (RS-MESTD-Basic Research (BR or ON)-173035)
- Имунопатогенетски и регулаторни механизми у аутоимунским болестима и хроничној инфламацији (RS-MESTD-Basic Research (BR or ON)-175038)
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.3920/BM2015.0159
ISSN: 1876-2883
PubMed: 26839070