Pituitary ACTH cells in female rats after neonatal treatment with SRIH-14
2009
Autori:
Milošević, VericaNestorović, Nataša
Terzić, Milica
Ristić, Nataša
Ajdžanović, Vladimir
Trifunović, Svetlana
Sekulić, Milka
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2009 by the Polish Histochemical et Cytochemical Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The prolonged effects of neonatal SRIH-14 treatment on pituitary ACTH cells were investigated. Neonatal female rats were injected subcutaneously with SRIH (20 μg/100g b.w.) every 12 hours for five consecutive days (3rd-7th day of life). Groups of rats were then killed at the juvenile (16th day), peripubertal (38th day) or adult (80th day) stage. ACTH cells were visualized using the peroxidase-antiperoxidase immunocytochemical procedure. Morphometry and stereology were used to evaluate the ACTH-immunoreactive cell volume and volume density. The histological and immunocytochemical characteristics of ACTH cells in neonatally treated females were changed in all examined periods. Thus, SRIH-14 induced significant (p<0.05) decreases of ACTH cell volume in juvenile, peripubertal and adult rats by 26%, 39% and 14%, respectively, in comparison to the corresponding controls. The volume density of ACTH cells was also diminished (by 31%; p<0.05) at the juvenile stage in comparison with the corresponding controls. In peripubertal and adult rats, the volume densities of ACTH cells were somewhat lower (17% and 14%, respectively), but the decreases did not reach statistical significance. These findings suggest that neonatal treatment with SRIH-14 exerts a marked prolonged inhibitory effect on ACTH cell
morphology extending to the adult period of life.
Ključne reči:
female rats; somatostatin; ACTH cells; development; immunocytochemistryIzvor:
Folia Histochemica et Cytobiologica, 2009, 47, 3, 479-484Finansiranje / projekti:
- Uticaj fitoestrogena, steroidnih i peptidnih hormona na ćelije neuroendokrinog sistema (RS-MESTD-MPN2006-2010-143007)
DOI: 10.2478/v10042-009-0104-1
ISSN: 0239-8508
PubMed: 20164035