Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
2016
Authors:
Prodanović, RadišaKorićanac, Goran
Vujanac, Ivan
Đorđević, Ana
Pantelić, Marija
Romić, Snježana
Stanimirović, Zoran
Kirovski, Danijela
Document Type:
Article (Published version)
,
© 2016 Elsevier Inc.
Metadata
Show full item recordAbstract:
We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic
fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups
based on their body condition score (BCS) as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Intravenous
glucose tolerance test (GTT) and liver biopsieswere carried out at day 10 before calving. Blood samples were collected
before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels
were determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measured
in the basal samples. The liver biopsieswere analyzed for total lipid content and protein expression of insulin receptor
beta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1).
Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides
and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development
of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of
the IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant
in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
Keywords:
dairy cows; fatty liver; CD36; Sterol regulatory element-binding protein-1Source:
Research in Veterinary Science, 2016, 107, 16-19Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
Related info:
DOI: 10.1016/j.rvsc.2016.04.007
ISSN: 0034-5288
PubMed: 27473969