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Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
dc.creator | Prodanović, Radiša | |
dc.creator | Korićanac, Goran | |
dc.creator | Vujanac, Ivan | |
dc.creator | Đorđević, Ana | |
dc.creator | Pantelić, Marija | |
dc.creator | Romić, Snježana | |
dc.creator | Stanimirović, Zoran | |
dc.creator | Kirovski, Danijela | |
dc.date.accessioned | 2023-09-28T08:10:57Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0034-5288 | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6085 | |
dc.description.abstract | We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Intravenous glucose tolerance test (GTT) and liver biopsieswere carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsieswere analyzed for total lipid content and protein expression of insulin receptor beta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41009/RS// | sr |
dc.relation.isversionof | https://radar.ibiss.bg.ac.rs/handle/123456789/6086 | |
dc.rights | restrictedAccess | sr |
dc.source | Research in Veterinary Science | sr |
dc.subject | dairy cows | sr |
dc.subject | fatty liver | sr |
dc.subject | CD36 | sr |
dc.subject | Sterol regulatory element-binding protein-1 | sr |
dc.title | Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2016 Elsevier Inc. | sr |
dc.citation.volume | 107 | |
dc.identifier.doi | 10.1016/j.rvsc.2016.04.007 | |
dc.identifier.pmid | 27473969 | |
dc.identifier.scopus | 2-s2.0-84969165526 | |
dc.identifier.wos | 000381541600003 | |
dc.citation.spage | 16 | |
dc.citation.epage | 19 | |
dc.type.version | publishedVersion | sr |
dc.citation.rank | M21 |