The effect of orchidectomy on thyroid C cells and bone histomorphometry in middle-aged rats
2007
Autori:
Filipović, BrankoŠošić-Jurjević, Branka
Ajdžanović, Vladimir
Trifunović, Svetlana
Manojlović-Stojanoski, Milica
Ristić, Nataša
Nestorović, Nataša
Milošević, Verica
Sekulić, Milka
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© Springer-Verlag 2007
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
This study was to evaluate the effect of androgen deficiency on thyroid immunoreactive C-cells and bone structure and function in a male orchidectomized middle-aged rat model. Fifteen-month-old male Wistar rats were divided into orchidectomized (Orx) and the sham-operated control (Sham) group. In the Orx group significant decreases (P < 0.05) were found in the volume of C cells (by 14%), their relative volume density (by 13%) and serum calcitonin concentration (by 54%) compared to the controls. Analyses of trabecular microarchitecture of the proximal tibia metaphysis showed that Orx induced marked decreases of cancellous bone area, trabecular thickness and trabecular number (by 52, 20 and 19% respectively; P < 0.05), whereas trabecular separation was increased by 27% (P < 0.05). In Orx rats, serum osteocalcin concentration was increased by 119% (P < 0.05), while serum calcium and phosphorus were 6 and 14% (P < 0.05) lower, respectively, compared to the levels in the Sham. In addition, urine calcium content was considerably higher (by 129%; P < 0.05) in Orx animals. These findings indicate that the androgen deficiency caused by Orx in middle-aged rats modulated the structure of C cells and diminished secretion of calcitonin. Histomorphometrical and biochemical analyses demonstrated a decrease of cancellous bone mass and increased bone turnover.
Ključne reči:
Orchidectomy; Thyroid C cells; Bone; Immunohistochemistry; HistomorphometryIzvor:
Histochemistry and Cell Biology, 2007, 128, 153-159Finansiranje / projekti:
- Uticaj fitoestrogena, steroidnih i peptidnih hormona na ćelije neuroendokrinog sistema (RS-MESTD-MPN2006-2010-143007)
DOI: 10.1007/s00418-007-0307-5
ISSN: 0948-6143
PubMed: 17609972