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Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats
dc.creator | Šošić-Jurjević, Branka | |
dc.creator | Filipović, Branko | |
dc.creator | Wirth, Eva Katrin | |
dc.creator | Živanović, Jasmina | |
dc.creator | Radulović, Niko | |
dc.creator | Janković, Snežana | |
dc.creator | Milošević, Verica | |
dc.creator | Köhrle, Josef | |
dc.date.accessioned | 2023-09-29T16:24:15Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0041-008X | |
dc.identifier.uri | http://radar.ibiss.bg.ac.rs/handle/123456789/6134 | |
dc.description.abstract | We previously reported that genistein (G) and daidzein (D) administered subcutaneously (10 mg/kg) induce changes in the angio-follicular units of the thyroid gland, reduce concentration of total thyroid hormones (TH) and increase thyrotropin (TSH) in serum of orchidectomized middle-aged (16-month-old) rats. To further investigate these effects, we now examined expression levels of the thyroglobulin (Tg), thyroperoxidase (Tpo), vascular endothelial growth factor A (Vegfa) and deiodinase type 1 (Dio 1) genes in the thyroid; in the pituitary, genes involved in TH feedback control (Tsh beta, Dio 1, Dio 2, Trh receptor); and in the liver and kidney, expression of T-3-activated genes Dio 1 and Spot 14, as well as transthyretin (Ttr), by quantitative real-time PCR. We also analyzed TPO-immunopositivity and immunofluorescence of T-4 bound to Tg, determined thyroid T-4 levels and measured deiodinase enzyme activities in examined organs. Decreased expression of Tg and Tpo genes (p < 0.05) correlated with immunohistochemical staining results, and together with decreased serum total T-4 levels, indicates decreased Tg and TH synthesis following treatments with both isoflavones. However, expression of Spot 14 (p < 0.05) gene in liver and kidney was up-regulated, and liver Dio 1 expression and activity (p < 0.05) increased. At the level of pituitary, no significant change in gene expression levels, or Dio 1 and 2 enzyme activities was observed. In conclusion, both G and D impaired Tg and TH synthesis, but at the same time increased tissue availability of TH in peripheral tissues of Orx middle-aged rats. | sr |
dc.language.iso | en | sr |
dc.publisher | Elsevier Inc | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173009/RS// | sr |
dc.rights | restrictedAccess | sr |
dc.source | Toxicology and Applied Pharmacology | sr |
dc.subject | isoflavones | sr |
dc.subject | genistein | sr |
dc.subject | daidzein | sr |
dc.subject | thyroid homeostasis | sr |
dc.subject | middle age | sr |
dc.subject | rat | sr |
dc.title | Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats | sr |
dc.type | article | sr |
dc.rights.license | ARR | sr |
dc.rights.holder | © 2014 Elsevier Inc. | sr |
dc.citation.issue | 2 | |
dc.citation.volume | 278 | |
dc.identifier.doi | 10.1016/j.taap.2014.04.018 | |
dc.identifier.pmid | 24793811 | |
dc.identifier.scopus | 2-s2.0-84905369528 | |
dc.identifier.wos | 000337335800005 | |
dc.citation.spage | 124 | |
dc.citation.epage | 134 | |
dc.type.version | publishedVersion | sr |
dc.citation.rank | M21 |