Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats
2023
Аутори:
Popović, DušankaKulaš, Jelena
Tucović, Dina
Popov Aleksandrov, Aleksandra
Glamočlija, Jasmina
Soković Bajić, Svetlana
Tolinacki, Maja
Golić, Nataša
Mirkov, Ivana
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2023 Institut Pasteur. Published by Elsevier Masson SAS.
Метаподаци
Приказ свих података о документуАпстракт:
Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigated
bacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiota
composition) in a healthy state and during pulmonary infection caused by the opportunistic fungus
Aspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DA
and AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterized
by increased alpha diversity indices and unchanged beta diversity. The relative abundance of
genera and species was increased in DA but decreased in AO rats during infection. Changes in lung
microbiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparate
response of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two rat
strains differentially susceptible to a subsequent inflammatory insult.
Кључне речи:
Aspergillus fumigatus; lung infection; inflammation; oxidative stress; rats; whole lung tissue microbiotaИзвор:
Microbes and Infection, 2023, 105186-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/6432
DOI: 10.1016/j.micinf.2023.105186
ISSN: 1286-4579