In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots
2011
Аутори:
Todorović, SlađanaBanjanac, Tijana
Bogdanović, Milica
Dragićević, Milan
Simonović, Ana
Giba, Zlatko
Grubišić, Dragoljub
Остала ауторства
Vinterhalter, DraganТип документа:
Конференцијски прилог (Објављена верзија)
,
© 2011 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Many chicory varieties (Cichorium intybus L., Asteraceae) are important agricultural and medicinal plants used throughout Europe. The bitter taste of chicory is associated with the presence of large quantities of sesquiterpene lactones. Some of the guanolides isolated from chicory, play role as antifeedants and phytoalexins, and possess cytotoxic activity towards cultured cancer cells, while the root extracts have anti-inflammatory and hepatoprotective activities. Hairy roots obtained by A. rhizogenes transformation grow fast and do not require hormones in the medium. The greatest advantage of hairy roots is that they often exhibit greater biosynthetic capacity for secondary metabolite
production compared to the mother plants. The main scope of our investigation was to optimize protocols for transformation of two chicory cultivars, C. intybus blue and C. intybus catalogna, with A. rhizogenes bearing 35S-GUS reporter vector and to establish optimal conditions for hairy roots growth. Transformation was confirmed in 7 C. intybus blue and in 5 C. intybus catalogna clones by PCR with GUS-specific primers. The GUS expression was confirmed in 6 C. intybus blue and 4 C. intybus catalogna clones by RT-PCR and histochemical GUS staining. Two of the transformed clones of C. intybus catalogna explained the greatest biomass production and growth potential. The established transformation protocol will be the basis for future planned transformation experiments and for the large-scale production of target secundary metabolites using hairy root cultures.
Кључне речи:
chicory; Hairy roots; sesquiterpene lactones; guanolides; A. rhizogenes transformationФинансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
- Plant Terpenoids for Human Health: a chemical and genomic approach to identify and produce bioactive compounds (EU-FP7-227448)
У:
- Vinterhalter D, editor. Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 79.