Ploidy profiling of Centaurium erythraea germplasm representing the diversity of the central Balkans: in vitro screening for highly productive diploid and tetraploid genotypes
2022
Аутори:
Filipović, BiljanaŠiler, Branislav
Banjanac, Tijana
Nestorović Živković, Jasmina
Skorić, Marijana
Krstić Milošević, Dijana
Mišić, Danijela
Остала ауторства
Milutinović, MilicaТип документа:
Конференцијски прилог (Објављена верзија)
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© 2022 by the Serbian Plant Physiology Society
Метаподаци
Приказ свих података о документуАпстракт:
Centaurium erythraea Rafn (common centaury) is an important medicinal plant species whose various health effects have been ascribed to its key specialized bioactive metabolites (mostly to secoiridoids and xanthones). Assessment of species diversity through the analysis of ploidy level and genetic variation is important for the selection of high-yielding genotypes for sustainable production of pharmacologically important specialized metabolites. In this work, we scored ploidy level of C. erythraea germplasm stored in ex situ seed collection containing seeds of more than 40 populations originating at locations across the central Balkan Peninsula. Furthermore, we selected two neighboring populations, one of the diploid and one of the tetraploid genome background, and compared growth parameters and productivity among and within diploid and tetraploid genotypes. Plants belonging to the diploid population showed different growth patterns as compared to tetraploid plants after one month of growth under in vitro conditions. To evaluate the content of iridoids (loganin and loganic acid), secoiridoids (secologanin, sweroside, gentiopicrin, and swertiamarin), and xanthones (demethyleustomin, methylbellidifolin, eustomin, and decussatin) in diploid and tetraploid genotypes, a targeted UHPLC/DAD/(±)HESI-MS2 analysis was performed for methanol extracts of shoots and roots, separately. One-month-old shoots of all diploid genotypes contained significantly higher amounts of total iridoids and xanthones than tetraploids, primarily due to high amounts of sweroside, decussatin, and methylbellidifolin. The observed variation both among and within diploid and tetraploid genotypes should be taken into consideration when estimating strategies for biotechnological improvement and for unraveling molecular background of specialized metabolites biosynthesis.
Кључне речи:
Centaurium erythraea; diploid; tetraploid; iridoids; xanthonesФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Milutinović M, editor. Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. Belgrade: Serbian Plant Physiology Society; 2022. p. 32.