Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats
2023
Аутори:
Popović, DušankaKulaš, Jelena
Tucović, Dina
Popov Aleksandrov, Aleksandra
Glamočlija, Jasmina
Soković Bajić, Svetlana
Tolinacki, Maja
Golić, Nataša
Mirkov, Ivana
Тип документа:
Чланак у часопису (Рецензирана верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Since the realization that the lungs are not sterile but are normally inhabited by various bacterial species, studies have been conducted to define healthy lung microbiota and to investigate whether it changes during lung diseases, infections, and inflammation. Using next-generation sequencing, we investigatedbacterial microbiota from whole lungs in two rat strains (previously shown to differ in gut microbiotacomposition) in a healthy state and during pulmonary infection caused by the opportunistic fungusAspergillus fumigatus. No differences in alpha diversity indices and microbial composition between DAand AO rats before infection were noted. Fungal infection caused dysbiosis in both rat strains, characterizedby increased alpha diversity indices and unchanged beta diversity. The relative abundance ofgenera and species was increased in DA but decreased in AO rats during infection. Changes in lungmicrobiota coincided with inflammation (in both rat strains) and oxidative stress (in DA rats). Disparateresponse of lung microbiota in DA and AO rats to pulmonary fungal infection might render these two ratstrains differentially susceptible to a subsequent inflammatory insult.
Напомена:
This is the peer reviewed version of the following article: Popovic D, Kulas J, Tucovic D, Popov Aleksandrov A, Glamoclija J, Sokovic Bajic S, Tolinacki M, Golic N, Mirkov I. Lung microbiota changes during pulmonary Aspergillus fumigatus infection in rats. Microbes Infect. 2023;105186. http://dx.doi.org/10.1016/j.micinf.2023.105186
Кључне речи:
Aspergillus fumigatus; lung infection; inflammation; oxidative stress; rats; whole lung tissue microbiotaИзвор:
Microbes and Infection, 2023, 105186-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
Повезане информације:
- Друга верзија
https://radar.ibiss.bg.ac.rs/handle/123456789/6162
DOI: 10.1016/j.micinf.2023.105186
ISSN: 1286-4579