Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures
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2024
Аутори:
Tovilović-Kovačević, Gordana![](/themes/MirageIBISS/images/orcid.png)
Ignjatović, Đurđica
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Krstić-Milošević, Dijana
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Tomić, Mirko
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Ćosić, Tatjana
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Тип документа:
Конференцијски прилог (Објављена верзија)
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© 2024 Institute for Genetic Engineering and Biotechnology, University of Sarajevo
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Приказ свих података о документуАпстракт:
Thymus pannonicus All. (Lamiaceae) is a perennial plant native to Central and Eastern Europe and Russia. The dried herb is traditionally used to treat mild respiratory and gastrointestinal illnesses, due to enrichment with monoterpenes and sesquiterpenes. Other secondary metabolites found in Thymus species, namely phenolic acids, flavonoids, flavone glucuronides, show numerous additional health-promoting effects (antioxidant, antidiabetic, anticancer). In this work, the antihepatoma activity of methanol extracts from in vitro shoot cultures of T. pannonicus citral chemotype on human HEPG2 hepatoma cells was evaluated. Two methanol extracts were investigated: E1 – derived from shoots grown on a medium without plant growth regulators and E2 – derived from shoots grown in the presence of the plant hormone indole-3-acetic acid (1mg/ml). Quantitative spectrophotometric analysis revealed that total phenolic and flavonoid contents were higher in E2 (540.4mg/g and 9.84mg/g, respectively) than in E1 (265.4mg/g and 6.52mg/g, respectively). Further analysis using HPLC identified rosmarinic acid (RA) as the dominant phenolic acid in both extracts, with E2 containing a slightly higher amount of RA than E1. The extracts reduced growth of HEPG2 cells in a dose- and time-dependent manner with moderate IC50 values (IC50(E1)=81.1µg/ml; IC50(E2)=77.8µg/ml, MTT, 96h), as shown by cell viability tests. Similarly, RA, used as a control, suppressed the growth of HEPG2 cells with an IC50=30.8µM (MTT, 96h). Flow cytometry of propidium iodide-stained cells showed that T. pannonicus extracts and RA arrested HEPG2 cells in the G2/M phase of the cell cycle, while fluorimetric measurement of cells loaded with dihydrorhodamine revealed mild elevation of intracellular ROS content induced by both extracts after 48h of treatment. Considering high content of RA in both extracts and similar antiproliferative effect of RA, E1 and E2 on HEPG2 cells, it could be proposed that RA is the active compound responsible for moderate antihepatoma activity of the investigated T. pannonicus extracts
Кључне речи:
Thymus pannonicus; in vitro shoot cultures; methanol extracts; rosmarinic acid; antihepatoma activity; antiproliferative effectФинансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
У:
- Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo; 2024. p. 27. (Genetics and Applications; Special edition).