TY  - JOUR
AU  - Milić, Ljiljana
AU  - Karamarković, Aleksandar
AU  - Popadić, Dušan
AU  - Šijački, Ana
AU  - Grigorov, Ilijana
AU  - Milošević, Emina
AU  - Ćuk, Vladica
AU  - Pesko, Predrag
PY  - 2019
UR  - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-019-4310-4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3362
AB  - Objective

Peptic ulcer disease is a condition in which an important role has infection with H. pylori. The most common complication of peptic ulcer is bleeding. The presence of H. pylori triggers local and systemic cytokine signaling which may affect processes such as healing, gastric or duodenal rupture, and carcinogenesis. In this study, we examined the concentrations of IL-1β, IL-6, IL-10, TNF, TGF-β and IL-17A in serum by enzyme immunoassay and their mRNA expressions in periulcer biopsies obtained from patients with bleeding peptic ulcer by means of real-time-PCR.
Results

We have shown that pro-inflammatory IL-6 and TNF concentrations in serum were significantly higher in patients who were infected with H. pylori, while the concentrations of TGF-β and IL-17A were significantly lower compared to non-infected subjects. IL-17A expression in periulcer mucosa was significantly higher in patients who were infected with H. pylori, while the expression of other cytokines, there was no significant difference compared to non-infected controls. Considering higher serum concentrations in non-infected subjects and higher IL-17A expression in mucosal tissue of infected patients, our data support the studies that found IL-17A has protective role in eradication of H. pylori infection in infected patients.
PB  - BioMed Central Ltd.
T2  - BMC Research Notes
T1  - Altered cytokine expression in Helicobacter pylori infected patients with bleeding duodenal ulcer
VL  - 12
DO  - 10.1186/s13104-019-4310-4
SP  - 278
ER  - 

@article{
author = "Milić, Ljiljana and Karamarković, Aleksandar and Popadić, Dušan and Šijački, Ana and Grigorov, Ilijana and Milošević, Emina and Ćuk, Vladica and Pesko, Predrag",
year = "2019",
abstract = "Objective

Peptic ulcer disease is a condition in which an important role has infection with H. pylori. The most common complication of peptic ulcer is bleeding. The presence of H. pylori triggers local and systemic cytokine signaling which may affect processes such as healing, gastric or duodenal rupture, and carcinogenesis. In this study, we examined the concentrations of IL-1β, IL-6, IL-10, TNF, TGF-β and IL-17A in serum by enzyme immunoassay and their mRNA expressions in periulcer biopsies obtained from patients with bleeding peptic ulcer by means of real-time-PCR.
Results

We have shown that pro-inflammatory IL-6 and TNF concentrations in serum were significantly higher in patients who were infected with H. pylori, while the concentrations of TGF-β and IL-17A were significantly lower compared to non-infected subjects. IL-17A expression in periulcer mucosa was significantly higher in patients who were infected with H. pylori, while the expression of other cytokines, there was no significant difference compared to non-infected controls. Considering higher serum concentrations in non-infected subjects and higher IL-17A expression in mucosal tissue of infected patients, our data support the studies that found IL-17A has protective role in eradication of H. pylori infection in infected patients.",
publisher = "BioMed Central Ltd.",
journal = "BMC Research Notes",
title = "Altered cytokine expression in Helicobacter pylori infected patients with bleeding duodenal ulcer",
volume = "12",
doi = "10.1186/s13104-019-4310-4",
pages = "278"
}

Milić, L., Karamarković, A., Popadić, D., Šijački, A., Grigorov, I., Milošević, E., Ćuk, V.,& Pesko, P.. (2019). Altered cytokine expression in Helicobacter pylori infected patients with bleeding duodenal ulcer. in BMC Research Notes
BioMed Central Ltd.., 12, 278.
https://doi.org/10.1186/s13104-019-4310-4

Milić L, Karamarković A, Popadić D, Šijački A, Grigorov I, Milošević E, Ćuk V, Pesko P. Altered cytokine expression in Helicobacter pylori infected patients with bleeding duodenal ulcer. in BMC Research Notes. 2019;12:278.
doi:10.1186/s13104-019-4310-4 .

Milić, Ljiljana, Karamarković, Aleksandar, Popadić, Dušan, Šijački, Ana, Grigorov, Ilijana, Milošević, Emina, Ćuk, Vladica, Pesko, Predrag, "Altered cytokine expression in Helicobacter pylori infected patients with bleeding duodenal ulcer" in BMC Research Notes, 12 (2019):278,
https://doi.org/10.1186/s13104-019-4310-4 . .

TY  - JOUR
AU  - Milić, Ljiljana
AU  - Grigorov, Ilijana
AU  - Krstić, Slobodan
AU  - Ćeranić, Miljan S.
AU  - Jovanović, Bojan
AU  - Stevanović, Jelena
AU  - Peško, Predrag
PY  - 2017
UR  - http://www.degruyter.com/view/j/jomb.2017.36.issue-1/jomb-2016-0016/jomb-2016-0016.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2558
AB  - Background: Intra-abdominal infection in secondary peritonitis drives as excessive production of inflammatory mediators and the development of systemic inflammatory response syndrome (SIRS) or sepsis. Finding a specific marker to distinguish SIRS from sepsis would be of immense clinical importance for the therapeutic approach. It is assumed that high-mobility group box 1 protein (HMGB1) could be such a marker. In this study, we examined the time course changes in the blood levels of HMGB1, C-reactive protein (CRP), procalcitonin (PCT) and serum amyloid A (SAA) in patients with secondary peritonitis who developed SIRS or sepsis.

Methods: In our study, we evaluated 100 patients with diffuse secondary peritonitis who developed SIRS or sepsis (SIRS and SEPSIS group) and 30 patients with inguinal hernia as a control group. Serum levels of HMGB1, CRP, PCT, and SAA were determined on admission in all the patients, and monitored daily in patients with peritonitis until discharge from hospital.

Results: Preoperative HMGB1, CRP, PCT and SAA levels were statistically highly significantly increased in patients with peritonitis compared to patients with inguinal hernia, and significantly higher in patients with sepsis compared to those with SIRS. All four inflammatory markers changed significantly during the follow-up. It is interesting that the patterns of change of HMGB1 and SAA over time were distinctive for SIRS and SEPSIS groups.

Conclusions: HMGB1 and SAA temporal patterns might be useful in distinguishing sepsis from noninfectious SIRS in secondary peritonitis.
T2  - Journal of Medical Biochemistry
T1  - Serum Level of HMGB1 Protein and Inflammatory Markers in Patients with Secondary Peritonitis: Time Course and the Association with Clinical Status
IS  - 1
VL  - 36
DO  - 10.1515/jomb-2016-0016
SP  - 44
EP  - 53
ER  - 

@article{
author = "Milić, Ljiljana and Grigorov, Ilijana and Krstić, Slobodan and Ćeranić, Miljan S. and Jovanović, Bojan and Stevanović, Jelena and Peško, Predrag",
year = "2017",
abstract = "Background: Intra-abdominal infection in secondary peritonitis drives as excessive production of inflammatory mediators and the development of systemic inflammatory response syndrome (SIRS) or sepsis. Finding a specific marker to distinguish SIRS from sepsis would be of immense clinical importance for the therapeutic approach. It is assumed that high-mobility group box 1 protein (HMGB1) could be such a marker. In this study, we examined the time course changes in the blood levels of HMGB1, C-reactive protein (CRP), procalcitonin (PCT) and serum amyloid A (SAA) in patients with secondary peritonitis who developed SIRS or sepsis.

Methods: In our study, we evaluated 100 patients with diffuse secondary peritonitis who developed SIRS or sepsis (SIRS and SEPSIS group) and 30 patients with inguinal hernia as a control group. Serum levels of HMGB1, CRP, PCT, and SAA were determined on admission in all the patients, and monitored daily in patients with peritonitis until discharge from hospital.

Results: Preoperative HMGB1, CRP, PCT and SAA levels were statistically highly significantly increased in patients with peritonitis compared to patients with inguinal hernia, and significantly higher in patients with sepsis compared to those with SIRS. All four inflammatory markers changed significantly during the follow-up. It is interesting that the patterns of change of HMGB1 and SAA over time were distinctive for SIRS and SEPSIS groups.

Conclusions: HMGB1 and SAA temporal patterns might be useful in distinguishing sepsis from noninfectious SIRS in secondary peritonitis.",
journal = "Journal of Medical Biochemistry",
title = "Serum Level of HMGB1 Protein and Inflammatory Markers in Patients with Secondary Peritonitis: Time Course and the Association with Clinical Status",
number = "1",
volume = "36",
doi = "10.1515/jomb-2016-0016",
pages = "44-53"
}

Milić, L., Grigorov, I., Krstić, S., Ćeranić, M. S., Jovanović, B., Stevanović, J.,& Peško, P.. (2017). Serum Level of HMGB1 Protein and Inflammatory Markers in Patients with Secondary Peritonitis: Time Course and the Association with Clinical Status. in Journal of Medical Biochemistry, 36(1), 44-53.
https://doi.org/10.1515/jomb-2016-0016

Milić L, Grigorov I, Krstić S, Ćeranić MS, Jovanović B, Stevanović J, Peško P. Serum Level of HMGB1 Protein and Inflammatory Markers in Patients with Secondary Peritonitis: Time Course and the Association with Clinical Status. in Journal of Medical Biochemistry. 2017;36(1):44-53.
doi:10.1515/jomb-2016-0016 .

Milić, Ljiljana, Grigorov, Ilijana, Krstić, Slobodan, Ćeranić, Miljan S., Jovanović, Bojan, Stevanović, Jelena, Peško, Predrag, "Serum Level of HMGB1 Protein and Inflammatory Markers in Patients with Secondary Peritonitis: Time Course and the Association with Clinical Status" in Journal of Medical Biochemistry, 36, no. 1 (2017):44-53,
https://doi.org/10.1515/jomb-2016-0016 . .