Oljača, Jasmina

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Potato minituber production by aeroponics: effects of plant origin and genotype

Broćić, Zoran; Oljača, Jasmina; Pantelić, Danijel; Rudić, Jelena; Poštić, Dobrivoj; Momčilović, Ivana

(Belgrade: University of Belgrade, Faculty of Agriculture, 2023)

TY  - CONF
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Poštić, Dobrivoj
AU  - Momčilović, Ivana
PY  - 2023
UR  - https://agrif.bg.ac.rs/uploads/files/strane/Fakultet/Izdavacka_delatnost/Zbornici_radova/XI%20Simpozijum%20sa%20medjunarodnim%20ucescem%20-%20Inovacije%20u%20ratarskoj%20i%20povrtarskoj%20proizvodnji.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6487
AB  - Aeroponics is modern, soilless technology for potato cultivation that promotes the production
of a large number of healthy minitubers (pre-basic seed potatoes). In aeroponics, roots and underground
stems (stolons) of potato plants grow inside closed boxes (modules), suspended in the fine mist of a
nutrient solution that recirculates through the system. As a result, a significant number of tubers larger
than 1 cm can be formed on stolons during the growing period. The aim of this study was to evaluate
the effects of the genotype and origin of planting material on the production of minituber in an aeroponic
facility in Guča, Serbia. Our study encompassed three potato cultivars: Cleopatra, Kennebec and
Désirée, and two types of planting material: acclimated microplants and plants originated from the
previous season’s minitubers. Plants were aeroponically cultivated in the 2019 season (late May-
December) with a planting density of 24 plants per m2 and ~14-day harvest intervals. The largest
number of minitubers per plant (19.9) was recorded for cultivar Désirée, followed by the Kennebec
(15.7) and Cleopatra (11.1). The average mass of minitubers of all the cultivars under consideration
was significantly higher in the plants originating from minitubers than in plants of in vitro origin. The
highest yield of minitubers, 10.27 kg m-2, was recorded for Kennebec plants of minituber origin.
Besides, the plants originated from minitubers steadily tuberized during the entire cultivation period
in the aeroponic facility.
AB  - Аеропоника је савремена технологија гајења биљака без земље која омогућава ефикасну
производњу здравог предосновног семенског кромпира у форми миникртола. У аеропонском
систему, коренови и столони биљака кромпира расту унутар затворених боксова (модула) у
магли капљица хранљивог раствора који циркулише кроз систем. Као резултат оваквог гајења,
значајан број кртола већих од 1 cm може се формирати на столонима биљака током вегетационог
периода. Циљ овог истраживања био је да се процене ефекти генотипа кромпира и порекла
садног материјала на производњу миникртола у објекту са аеропоником у Гучи, Србија. Наша
студија је обухватила три сорте кромпира: Cleopatra, Kennebec и Désirée, и две врсте садног
материјала: аклиматизоване микробиљке и биљке добијене из миникртола (род из претходне
сезоне). Биљке су узгајане аеропонски у сезони 2019. (крај маја-децембар) са густином садње
од 24 биљке по m2 и интервалима жетве од ~14 дана. Највећи број миникртола по биљци (19,9)
формирала је сорта Désirée, затим Kennebec (15,7) и Cleopatra (11,1). Просечна маса миникртола
свих испитиваних сорти била је значајно већа код биљака добијених из миникртола него код
биљака in vitro порекла. Највећи принос миникртола, 10,27 kg m-2, установљен је код биљака
сорте Kennebec узгојених из миникртола. Поред тога, биљке пореклом из миникртола су постојано
формирале кртоле током читавог периода гајења у аеропонском систему.
PB  - Belgrade: University of Belgrade, Faculty of Agriculture
C3  - Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia
T1  - Potato minituber production by aeroponics: effects of plant origin and genotype
T1  - Аеропонска производња миникртола кромпира: утицај порекла садног материјала и генотипа
SP  - 31
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6487
ER  - 
@conference{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Poštić, Dobrivoj and Momčilović, Ivana",
year = "2023",
abstract = "Aeroponics is modern, soilless technology for potato cultivation that promotes the production
of a large number of healthy minitubers (pre-basic seed potatoes). In aeroponics, roots and underground
stems (stolons) of potato plants grow inside closed boxes (modules), suspended in the fine mist of a
nutrient solution that recirculates through the system. As a result, a significant number of tubers larger
than 1 cm can be formed on stolons during the growing period. The aim of this study was to evaluate
the effects of the genotype and origin of planting material on the production of minituber in an aeroponic
facility in Guča, Serbia. Our study encompassed three potato cultivars: Cleopatra, Kennebec and
Désirée, and two types of planting material: acclimated microplants and plants originated from the
previous season’s minitubers. Plants were aeroponically cultivated in the 2019 season (late May-
December) with a planting density of 24 plants per m2 and ~14-day harvest intervals. The largest
number of minitubers per plant (19.9) was recorded for cultivar Désirée, followed by the Kennebec
(15.7) and Cleopatra (11.1). The average mass of minitubers of all the cultivars under consideration
was significantly higher in the plants originating from minitubers than in plants of in vitro origin. The
highest yield of minitubers, 10.27 kg m-2, was recorded for Kennebec plants of minituber origin.
Besides, the plants originated from minitubers steadily tuberized during the entire cultivation period
in the aeroponic facility., Аеропоника је савремена технологија гајења биљака без земље која омогућава ефикасну
производњу здравог предосновног семенског кромпира у форми миникртола. У аеропонском
систему, коренови и столони биљака кромпира расту унутар затворених боксова (модула) у
магли капљица хранљивог раствора који циркулише кроз систем. Као резултат оваквог гајења,
значајан број кртола већих од 1 cm може се формирати на столонима биљака током вегетационог
периода. Циљ овог истраживања био је да се процене ефекти генотипа кромпира и порекла
садног материјала на производњу миникртола у објекту са аеропоником у Гучи, Србија. Наша
студија је обухватила три сорте кромпира: Cleopatra, Kennebec и Désirée, и две врсте садног
материјала: аклиматизоване микробиљке и биљке добијене из миникртола (род из претходне
сезоне). Биљке су узгајане аеропонски у сезони 2019. (крај маја-децембар) са густином садње
од 24 биљке по m2 и интервалима жетве од ~14 дана. Највећи број миникртола по биљци (19,9)
формирала је сорта Désirée, затим Kennebec (15,7) и Cleopatra (11,1). Просечна маса миникртола
свих испитиваних сорти била је значајно већа код биљака добијених из миникртола него код
биљака in vitro порекла. Највећи принос миникртола, 10,27 kg m-2, установљен је код биљака
сорте Kennebec узгојених из миникртола. Поред тога, биљке пореклом из миникртола су постојано
формирале кртоле током читавог периода гајења у аеропонском систему.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia",
title = "Potato minituber production by aeroponics: effects of plant origin and genotype, Аеропонска производња миникртола кромпира: утицај порекла садног материјала и генотипа",
pages = "31-32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6487"
}
Broćić, Z., Oljača, J., Pantelić, D., Rudić, J., Poštić, D.,& Momčilović, I.. (2023). Potato minituber production by aeroponics: effects of plant origin and genotype. in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Agriculture., 31-32.
https://hdl.handle.net/21.15107/rcub_ibiss_6487
Broćić Z, Oljača J, Pantelić D, Rudić J, Poštić D, Momčilović I. Potato minituber production by aeroponics: effects of plant origin and genotype. in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia. 2023;:31-32.
https://hdl.handle.net/21.15107/rcub_ibiss_6487 .
Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Poštić, Dobrivoj, Momčilović, Ivana, "Potato minituber production by aeroponics: effects of plant origin and genotype" in Book of abstracts: 11th Symposium with International Participation Innovations in Field and Vegetable Crops Production; 2023 Oct 12-13;  Belgrade, Serbia (2023):31-32,
https://hdl.handle.net/21.15107/rcub_ibiss_6487 .

Effects of cultivar and plant origin on the aeroponic production of potato minitubers

Broćić, Zoran; Oljača, Jasmina; Pantelić, Danijel; Rudić, Jelena; Poštić, Dobrivoj; Momčilović, Ivana

(Banja Luka: University of Banja Luka, Faculty of Agriculture, 2023)

TY  - CONF
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Poštić, Dobrivoj
AU  - Momčilović, Ivana
PY  - 2023
UR  - https://agrores.agro.unibl.org/wp-content/uploads/2023/05/Book-of-Proceedings-AgroReS-2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6488
AB  - Aeroponics is eco-friendly, soilless technology for the cultivation of vegetable plants that can
be used for the production of pathogen-free pre-basic seed potato, namely minitubers. In
aeroponic modules, the underground parts of potato plants grow suspended in the mist of finely
dispersed nutrient solution to produce tubers, while the shoots grow above the module under
greenhouse conditions. This study aimed to evaluate the effects of the cultivar and origin of planting material on the minituber production in an aeroponic faility in Guča, Serbia. Two potato cultivars, Sinora and Agria, and two types of planting material, acclimated microplants
and plants originating from sprouted minitubers, were used in the study. Plants were grown in
the 2019 season with a planting density of 24 plants per m2 and ~14-day harvest intervals.
Agria plants of both plant origins steadily tuberized during most of the cultivation period and
formed a significantly larger number of minitubers (13.61) compared to cultivar Sinora (3.35),
which quickly completed the growth cycle. In both investigated cultivars, the mass of formed
minitubers was significantly higher in the plants originating from minitubers (18.80 g)
compared to plants of in vitro origin (9.04 g). Agria plants of minituber origin produced the
heaviest minitubers (19.05 g), while Sinora plants of in vitro origin formed the least heavy
tubers (5.29 g). The highest yield of minitubers, 6.26 kg m-2, was recorded for Agria plants of
minituber origin. The results of our study suggest that both plant origin and potato genotype
significantly affect minituber production in aeroponics.
PB  - Banja Luka: University of Banja Luka, Faculty of Agriculture
C3  - Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina
T1  - Effects of cultivar and plant origin on the aeroponic production of potato minitubers
DO  - 10.7251/ZARS2301009B
SP  - 9
EP  - 18
ER  - 
@conference{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Poštić, Dobrivoj and Momčilović, Ivana",
year = "2023",
abstract = "Aeroponics is eco-friendly, soilless technology for the cultivation of vegetable plants that can
be used for the production of pathogen-free pre-basic seed potato, namely minitubers. In
aeroponic modules, the underground parts of potato plants grow suspended in the mist of finely
dispersed nutrient solution to produce tubers, while the shoots grow above the module under
greenhouse conditions. This study aimed to evaluate the effects of the cultivar and origin of planting material on the minituber production in an aeroponic faility in Guča, Serbia. Two potato cultivars, Sinora and Agria, and two types of planting material, acclimated microplants
and plants originating from sprouted minitubers, were used in the study. Plants were grown in
the 2019 season with a planting density of 24 plants per m2 and ~14-day harvest intervals.
Agria plants of both plant origins steadily tuberized during most of the cultivation period and
formed a significantly larger number of minitubers (13.61) compared to cultivar Sinora (3.35),
which quickly completed the growth cycle. In both investigated cultivars, the mass of formed
minitubers was significantly higher in the plants originating from minitubers (18.80 g)
compared to plants of in vitro origin (9.04 g). Agria plants of minituber origin produced the
heaviest minitubers (19.05 g), while Sinora plants of in vitro origin formed the least heavy
tubers (5.29 g). The highest yield of minitubers, 6.26 kg m-2, was recorded for Agria plants of
minituber origin. The results of our study suggest that both plant origin and potato genotype
significantly affect minituber production in aeroponics.",
publisher = "Banja Luka: University of Banja Luka, Faculty of Agriculture",
journal = "Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina",
title = "Effects of cultivar and plant origin on the aeroponic production of potato minitubers",
doi = "10.7251/ZARS2301009B",
pages = "9-18"
}
Broćić, Z., Oljača, J., Pantelić, D., Rudić, J., Poštić, D.,& Momčilović, I.. (2023). Effects of cultivar and plant origin on the aeroponic production of potato minitubers. in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina
Banja Luka: University of Banja Luka, Faculty of Agriculture., 9-18.
https://doi.org/10.7251/ZARS2301009B
Broćić Z, Oljača J, Pantelić D, Rudić J, Poštić D, Momčilović I. Effects of cultivar and plant origin on the aeroponic production of potato minitubers. in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina. 2023;:9-18.
doi:10.7251/ZARS2301009B .
Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Poštić, Dobrivoj, Momčilović, Ivana, "Effects of cultivar and plant origin on the aeroponic production of potato minitubers" in Book of proceedings: 12th International Symposium on Agricultural Sciences: AgroReS 2023; 2023 May 24-26; Trebinje, Bosnia and Herzegovina (2023):9-18,
https://doi.org/10.7251/ZARS2301009B . .

Potato aeroponics: effects of cultivar and plant origin on minituber production

Broćić, Zoran; Oljača, Jasmina; Pantelić, Danijel; Rudić, Jelena; Momčilović, Ivana

(Basel: MDPI, 2022)

TY  - JOUR
AU  - Broćić, Zoran
AU  - Oljača, Jasmina
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Momčilović, Ivana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5122
AB  - Aeroponics is a modern and soilless technology that is used for the efficient production of pre-basic seed potatoes, namely minitubers. This study aimed to evaluate the effects of the cultivar and type of planting material on the production of minitubers in the aeroponic facility in Guča, Serbia, at short, 7-day harvest intervals. Although aeroponic cultivation prolonged the vegetative cycle in all five investigated cultivars, the dynamics of minituber formation varied between genotypes. Two early maturing cultivars, Cleopatra and Sinora, quickly completed the vegetative cycle and formed a small number of minitubers, while the medium-late to late cultivars, Kennebec and Agria, steadily tuberized during the entire cultivation period in the aeroponic facility. The type of planting material affected the dynamics of minituber formation in three investigated cultivars. Sinora, Cleopatra, and Désirée’s plants of in vitro origin reached the final number of minitubers and the vines started senescing much earlier than plants of minituber origin. Kennebec and Agria plants of in vitro origin produced the largest number of minitubers (53.8–54.5) and showed the highest yield (9.8–10.5 kg m−2) during the cultivation period, while the heaviest minitubers were formed by Sinora plants of minituber origin (15.48 g). In addition, the temperature during pre-harvest periods significantly affected the number of tubers at harvests in Kennebec and Agria, and minituber mass in Désirée.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Potato aeroponics: effects of cultivar and plant origin on minituber production
IS  - 10
VL  - 8
DO  - 10.3390/horticulturae8100915
SP  - 915
ER  - 
@article{
author = "Broćić, Zoran and Oljača, Jasmina and Pantelić, Danijel and Rudić, Jelena and Momčilović, Ivana",
year = "2022",
abstract = "Aeroponics is a modern and soilless technology that is used for the efficient production of pre-basic seed potatoes, namely minitubers. This study aimed to evaluate the effects of the cultivar and type of planting material on the production of minitubers in the aeroponic facility in Guča, Serbia, at short, 7-day harvest intervals. Although aeroponic cultivation prolonged the vegetative cycle in all five investigated cultivars, the dynamics of minituber formation varied between genotypes. Two early maturing cultivars, Cleopatra and Sinora, quickly completed the vegetative cycle and formed a small number of minitubers, while the medium-late to late cultivars, Kennebec and Agria, steadily tuberized during the entire cultivation period in the aeroponic facility. The type of planting material affected the dynamics of minituber formation in three investigated cultivars. Sinora, Cleopatra, and Désirée’s plants of in vitro origin reached the final number of minitubers and the vines started senescing much earlier than plants of minituber origin. Kennebec and Agria plants of in vitro origin produced the largest number of minitubers (53.8–54.5) and showed the highest yield (9.8–10.5 kg m−2) during the cultivation period, while the heaviest minitubers were formed by Sinora plants of minituber origin (15.48 g). In addition, the temperature during pre-harvest periods significantly affected the number of tubers at harvests in Kennebec and Agria, and minituber mass in Désirée.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Potato aeroponics: effects of cultivar and plant origin on minituber production",
number = "10",
volume = "8",
doi = "10.3390/horticulturae8100915",
pages = "915"
}
Broćić, Z., Oljača, J., Pantelić, D., Rudić, J.,& Momčilović, I.. (2022). Potato aeroponics: effects of cultivar and plant origin on minituber production. in Horticulturae
Basel: MDPI., 8(10), 915.
https://doi.org/10.3390/horticulturae8100915
Broćić Z, Oljača J, Pantelić D, Rudić J, Momčilović I. Potato aeroponics: effects of cultivar and plant origin on minituber production. in Horticulturae. 2022;8(10):915.
doi:10.3390/horticulturae8100915 .
Broćić, Zoran, Oljača, Jasmina, Pantelić, Danijel, Rudić, Jelena, Momčilović, Ivana, "Potato aeroponics: effects of cultivar and plant origin on minituber production" in Horticulturae, 8, no. 10 (2022):915,
https://doi.org/10.3390/horticulturae8100915 . .
11
6
6

Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants

Rudić, Jelena; Pantelić, Danijel; Oljača, Jasmina; Momčilović, Ivana

(Basel: MDPI, 2022)

TY  - JOUR
AU  - Rudić, Jelena
AU  - Pantelić, Danijel
AU  - Oljača, Jasmina
AU  - Momčilović, Ivana
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/5/372
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4979
AB  - Potato is a globally important, highly heat-susceptible crop species. We investigated the effects of prolonged exposure to elevated temperatures and exogenous salicylic acid (SA) on microplant growth and heat-shock response (HSR) in three unrelated potato genotypes/cultivars. Long-term exposure to 29 °C (mild heat stress) caused a significant reduction in the number of surviving explants and shoot morphometric parameters in heat-sensitive genotypes, while exposure to 26 °C (warming) caused only a decline in shoot growth. Interestingly, 26 °C-temperature treatment stimulated root growth in some investigated genotypes, indicating a difference between favorable temperatures for potato shoot and root growth. SA showed a protective effect regarding potato shoot growth at 26 °C. At 29 °C, this effect was genotype-dependent. SA did not affect the number of roots and inhibited root elongation at all temperature treatments, indicating the difference between shoot and root responses to applied SA concentration. Although HSR is mainly considered rapid and short-lived, elevated transcript levels of most investigated HSFs and HSPs were detected after three weeks of heat stress. Besides, two StHSFs and StHSP21 showed elevated expression at 26 °C, indicating extreme potato heat-susceptibility and significance of HSR during prolonged warming. SA effects on HSFs and HSPs expression were minor and alterable.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants
IS  - 5
VL  - 8
DO  - 10.3390/horticulturae8050372
SP  - 372
ER  - 
@article{
author = "Rudić, Jelena and Pantelić, Danijel and Oljača, Jasmina and Momčilović, Ivana",
year = "2022",
abstract = "Potato is a globally important, highly heat-susceptible crop species. We investigated the effects of prolonged exposure to elevated temperatures and exogenous salicylic acid (SA) on microplant growth and heat-shock response (HSR) in three unrelated potato genotypes/cultivars. Long-term exposure to 29 °C (mild heat stress) caused a significant reduction in the number of surviving explants and shoot morphometric parameters in heat-sensitive genotypes, while exposure to 26 °C (warming) caused only a decline in shoot growth. Interestingly, 26 °C-temperature treatment stimulated root growth in some investigated genotypes, indicating a difference between favorable temperatures for potato shoot and root growth. SA showed a protective effect regarding potato shoot growth at 26 °C. At 29 °C, this effect was genotype-dependent. SA did not affect the number of roots and inhibited root elongation at all temperature treatments, indicating the difference between shoot and root responses to applied SA concentration. Although HSR is mainly considered rapid and short-lived, elevated transcript levels of most investigated HSFs and HSPs were detected after three weeks of heat stress. Besides, two StHSFs and StHSP21 showed elevated expression at 26 °C, indicating extreme potato heat-susceptibility and significance of HSR during prolonged warming. SA effects on HSFs and HSPs expression were minor and alterable.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants",
number = "5",
volume = "8",
doi = "10.3390/horticulturae8050372",
pages = "372"
}
Rudić, J., Pantelić, D., Oljača, J.,& Momčilović, I.. (2022). Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants. in Horticulturae
Basel: MDPI., 8(5), 372.
https://doi.org/10.3390/horticulturae8050372
Rudić J, Pantelić D, Oljača J, Momčilović I. Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants. in Horticulturae. 2022;8(5):372.
doi:10.3390/horticulturae8050372 .
Rudić, Jelena, Pantelić, Danijel, Oljača, Jasmina, Momčilović, Ivana, "Effects of Elevated Temperature and Salicylic Acid on Heat Shock Response and Growth of Potato Microplants" in Horticulturae, 8, no. 5 (2022):372,
https://doi.org/10.3390/horticulturae8050372 . .
4
4
4

Production of High-Quality Seed Potato by Aeroponics

Villa, Pedro; Broćić, Zoran; Momčilović, Ivana; Poštić, Dobrivoj; Oljača, Jasmina; Veljković, Biljana

(New York : Nova Science Publishers Inc., 2021)

TY  - CHAP
AU  - Broćić, Zoran
AU  - Momčilović, Ivana
AU  - Poštić, Dobrivoj
AU  - Oljača, Jasmina
AU  - Veljković, Biljana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4471
AB  - Commercial production of potato, Solanum tuberosum L., is based on vegetative propagation by seed tubers. High-quality seed material is obtained from potato plants grown from minitubers (pre-basic seed potatoes). Likewise, minitubers are produced by acclimation and growth on soil or solid substrate of previously in vitro-propagated, virus-free microplants or microtubers. Aeroponics is a modern, soilless technique for minitubers’ production. In the aeroponic cultivation system, foliage is grown under conventional conditions, while the underground stems and roots of potato plants are located in a dark chamber, module, suspended in the air, and supplied with water and nutrients through a nutrient solution dispersed in the form of fine mist particles. Minitubers (tubers of 5-25 mm in size) are produced on underground stems, namely stolons. Potato minituber propagation in aeroponics has significant advantages over other used systems or techniques. This system enables the production of a high number of minitubers per plant during the production cycle that can usually be repeated during the year. Besides, successive harvesting allows minitubers to reach the desired, uniform size. Tubers grown in an aeroponic system are well-protected from pests and soil-borne diseases. Due to the recirculation of nutrient solution, efficient usage of space, and minimal environmental pollution, aeroponics enables the production of minitubers in an environmentally friendly manner. This chapter summarizes the current knowledge of the aeroponic production of potatoes. The advantages and deficiencies of this interesting production technique are also discussed.
PB  - New York : Nova Science Publishers Inc.
T2  - The Potato Crop: Management, Production, and Food Security
T1  - Production of High-Quality Seed Potato by Aeroponics
DO  - 10.52305/RHLO1469
SP  - 25
EP  - 59
ER  - 
@inbook{
editor = "Villa, Pedro",
author = "Broćić, Zoran and Momčilović, Ivana and Poštić, Dobrivoj and Oljača, Jasmina and Veljković, Biljana",
year = "2021",
abstract = "Commercial production of potato, Solanum tuberosum L., is based on vegetative propagation by seed tubers. High-quality seed material is obtained from potato plants grown from minitubers (pre-basic seed potatoes). Likewise, minitubers are produced by acclimation and growth on soil or solid substrate of previously in vitro-propagated, virus-free microplants or microtubers. Aeroponics is a modern, soilless technique for minitubers’ production. In the aeroponic cultivation system, foliage is grown under conventional conditions, while the underground stems and roots of potato plants are located in a dark chamber, module, suspended in the air, and supplied with water and nutrients through a nutrient solution dispersed in the form of fine mist particles. Minitubers (tubers of 5-25 mm in size) are produced on underground stems, namely stolons. Potato minituber propagation in aeroponics has significant advantages over other used systems or techniques. This system enables the production of a high number of minitubers per plant during the production cycle that can usually be repeated during the year. Besides, successive harvesting allows minitubers to reach the desired, uniform size. Tubers grown in an aeroponic system are well-protected from pests and soil-borne diseases. Due to the recirculation of nutrient solution, efficient usage of space, and minimal environmental pollution, aeroponics enables the production of minitubers in an environmentally friendly manner. This chapter summarizes the current knowledge of the aeroponic production of potatoes. The advantages and deficiencies of this interesting production technique are also discussed.",
publisher = "New York : Nova Science Publishers Inc.",
journal = "The Potato Crop: Management, Production, and Food Security",
booktitle = "Production of High-Quality Seed Potato by Aeroponics",
doi = "10.52305/RHLO1469",
pages = "25-59"
}
Villa, P., Broćić, Z., Momčilović, I., Poštić, D., Oljača, J.,& Veljković, B.. (2021). Production of High-Quality Seed Potato by Aeroponics. in The Potato Crop: Management, Production, and Food Security
New York : Nova Science Publishers Inc.., 25-59.
https://doi.org/10.52305/RHLO1469
Villa P, Broćić Z, Momčilović I, Poštić D, Oljača J, Veljković B. Production of High-Quality Seed Potato by Aeroponics. in The Potato Crop: Management, Production, and Food Security. 2021;:25-59.
doi:10.52305/RHLO1469 .
Villa, Pedro, Broćić, Zoran, Momčilović, Ivana, Poštić, Dobrivoj, Oljača, Jasmina, Veljković, Biljana, "Production of High-Quality Seed Potato by Aeroponics" in The Potato Crop: Management, Production, and Food Security (2021):25-59,
https://doi.org/10.52305/RHLO1469 . .
7

Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance

Kovačević, Dušan; Pantelić, Danijel; Rudić, Jelena; Oljača, Jasmina; Simonović, Ana; Broćić, Zoran; Momčilović, Ivana

(Sarajevo: University of East Sarajevo, Faculty of Agriculture, 2018)

TY  - CONF
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Oljača, Jasmina
AU  - Simonović, Ana
AU  - Broćić, Zoran
AU  - Momčilović, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4348
AB  - Potato is a worldwide food crop and ranked as the world’s fourth most consumed food after rice, wheat, and maize. Belongs to cool-season crops, wherefore high temperatures have negative impact on economic yield and tuber quality. High temperatures affect morphogenesis of potato plants grown in the field as well as in controlled artificial environment. First step in the present study was to investigate the effects of high-temperature on tuberization in two in vitro potato cultivars, Désirée and Festival. Furthermore, we exposed these two tuberizing potato cultivars to mildly elevated temperatures (30/24°C, day/night) for one weeks and compared with plants grown under optimal conditions (20/18°C), using an ex vitro system. In our work, we also tested seven potato cultivars in the field under most favorable condition (~22oC) and under high-temperature condition (~35oC). We have investigated high-temperatures induced expression of translation elongation factor (eEF1A) in all potato cultivars growth in different conditions. eEF1A is a group of highly conserved proteins that play a central role in the elongation phase of protein biosynthesis, and also shows chaperone activity in protecting other proteins from aggregation caused by high temperatures. Using 1D-PAGE and Western blott analysis, we are determined significant differences in accumulation of eEF1A among potato cultivars. Also, qPCR revealed that elevated temperature had a profound impact on tuber mRNA expression of eEF1A isoforms. Our research shows that potato eEF1A proteins may play an important role in the process of tuberization under high-temperature conditions and development of heat tolerance by acting as molecular chaperone.
PB  - Sarajevo: University of East Sarajevo, Faculty of Agriculture
C3  - Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina
T1  - Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance
SP  - 335
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4348
ER  - 
@conference{
editor = "Kovačević, Dušan",
author = "Pantelić, Danijel and Rudić, Jelena and Oljača, Jasmina and Simonović, Ana and Broćić, Zoran and Momčilović, Ivana",
year = "2018",
abstract = "Potato is a worldwide food crop and ranked as the world’s fourth most consumed food after rice, wheat, and maize. Belongs to cool-season crops, wherefore high temperatures have negative impact on economic yield and tuber quality. High temperatures affect morphogenesis of potato plants grown in the field as well as in controlled artificial environment. First step in the present study was to investigate the effects of high-temperature on tuberization in two in vitro potato cultivars, Désirée and Festival. Furthermore, we exposed these two tuberizing potato cultivars to mildly elevated temperatures (30/24°C, day/night) for one weeks and compared with plants grown under optimal conditions (20/18°C), using an ex vitro system. In our work, we also tested seven potato cultivars in the field under most favorable condition (~22oC) and under high-temperature condition (~35oC). We have investigated high-temperatures induced expression of translation elongation factor (eEF1A) in all potato cultivars growth in different conditions. eEF1A is a group of highly conserved proteins that play a central role in the elongation phase of protein biosynthesis, and also shows chaperone activity in protecting other proteins from aggregation caused by high temperatures. Using 1D-PAGE and Western blott analysis, we are determined significant differences in accumulation of eEF1A among potato cultivars. Also, qPCR revealed that elevated temperature had a profound impact on tuber mRNA expression of eEF1A isoforms. Our research shows that potato eEF1A proteins may play an important role in the process of tuberization under high-temperature conditions and development of heat tolerance by acting as molecular chaperone.",
publisher = "Sarajevo: University of East Sarajevo, Faculty of Agriculture",
journal = "Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina",
title = "Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance",
pages = "335",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4348"
}
Kovačević, D., Pantelić, D., Rudić, J., Oljača, J., Simonović, A., Broćić, Z.,& Momčilović, I.. (2018). Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance. in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina
Sarajevo: University of East Sarajevo, Faculty of Agriculture., 335.
https://hdl.handle.net/21.15107/rcub_ibiss_4348
Kovačević D, Pantelić D, Rudić J, Oljača J, Simonović A, Broćić Z, Momčilović I. Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance. in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina. 2018;:335.
https://hdl.handle.net/21.15107/rcub_ibiss_4348 .
Kovačević, Dušan, Pantelić, Danijel, Rudić, Jelena, Oljača, Jasmina, Simonović, Ana, Broćić, Zoran, Momčilović, Ivana, "Heat-induced Expression of Protein Synthesis Elongation Factor 1A Associated with Potato Heat Tolerance" in Book of abstracts: IX International Scientific Agriculture Symposium: Agrosym 2018; 2018 Oct 4-7; Jahorina, Republika Srpska, Bosnia and Herzegovina (2018):335,
https://hdl.handle.net/21.15107/rcub_ibiss_4348 .

Prospective protein markers for selection of heat tolerant potato cultivars

Oljača, Jasmina; Broćič, Zoran; Pantelić, Danijel; Rudić, Jelena; Momčilović, Ivana

(Sarajevo: University of East Sarajevo, Faculty of Agriculture, 2018)

TY  - CONF
AU  - Oljača, Jasmina
AU  - Broćič, Zoran
AU  - Pantelić, Danijel
AU  - Rudić, Jelena
AU  - Momčilović, Ivana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4349
AB  - Most of the commercially important cultivars of potato are well adapted to cool
climates, whilst adversely affected by high temperatures. In order to develop a procedure for efficient screening of potato genotypes regarding heat tolerance, we investigated expression and accumulation of heat stress-related HSP17.6, HSP21, HSP101 and eEF1A proteins in various potato cultivars both in a controlled environment and field trials. Potato plants were grown in a climate-controlled chamber and exposed to prolonged heat stress (HS) in the stages of tuber initiation and early tuber bulking. Tuberization parameters were measured and prospective protein markers analyzed in collected leaf and minituber samples. In a field experiment, the potato was grown in the irrigated field in Zemun Polje (Serbia) and leaf samples for protein analyses were collected after high-temperature incidents in two extremely
hot years (2011 and 2012). Besides, relevant growth and yield parameters were measured each year. Positive, linear correlation was determined between yield per plot and accumulation of HSP17.6, HSP101 or eEF1A under HS in examined potato cultivars, while negative correlation was determined between the height of primary shoots, as well as aboveground biomass, and accumulation of these three proteins. In other words, potato genotypes which accumulated higher amounts of HSP17.6, HSP101, and eEF1A under HS in the field, also had shorter primary shoots, lower above-ground biomass, and higher tuber yield. Our results indicated that among investigated proteins, HSP17.6, HSP101, and eEF1A might be considered as prospective protein markers for selection of heat tolerant potato genotypes.
PB  - Sarajevo: University of East Sarajevo, Faculty of Agriculture
C3  - IX International Scientific Agriculture Symposium “Agrosym 2018”
T1  - Prospective protein markers for selection of heat tolerant potato cultivars
SP  - 340
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4349
ER  - 
@conference{
author = "Oljača, Jasmina and Broćič, Zoran and Pantelić, Danijel and Rudić, Jelena and Momčilović, Ivana",
year = "2018",
abstract = "Most of the commercially important cultivars of potato are well adapted to cool
climates, whilst adversely affected by high temperatures. In order to develop a procedure for efficient screening of potato genotypes regarding heat tolerance, we investigated expression and accumulation of heat stress-related HSP17.6, HSP21, HSP101 and eEF1A proteins in various potato cultivars both in a controlled environment and field trials. Potato plants were grown in a climate-controlled chamber and exposed to prolonged heat stress (HS) in the stages of tuber initiation and early tuber bulking. Tuberization parameters were measured and prospective protein markers analyzed in collected leaf and minituber samples. In a field experiment, the potato was grown in the irrigated field in Zemun Polje (Serbia) and leaf samples for protein analyses were collected after high-temperature incidents in two extremely
hot years (2011 and 2012). Besides, relevant growth and yield parameters were measured each year. Positive, linear correlation was determined between yield per plot and accumulation of HSP17.6, HSP101 or eEF1A under HS in examined potato cultivars, while negative correlation was determined between the height of primary shoots, as well as aboveground biomass, and accumulation of these three proteins. In other words, potato genotypes which accumulated higher amounts of HSP17.6, HSP101, and eEF1A under HS in the field, also had shorter primary shoots, lower above-ground biomass, and higher tuber yield. Our results indicated that among investigated proteins, HSP17.6, HSP101, and eEF1A might be considered as prospective protein markers for selection of heat tolerant potato genotypes.",
publisher = "Sarajevo: University of East Sarajevo, Faculty of Agriculture",
journal = "IX International Scientific Agriculture Symposium “Agrosym 2018”",
title = "Prospective protein markers for selection of heat tolerant potato cultivars",
pages = "340",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4349"
}
Oljača, J., Broćič, Z., Pantelić, D., Rudić, J.,& Momčilović, I.. (2018). Prospective protein markers for selection of heat tolerant potato cultivars. in IX International Scientific Agriculture Symposium “Agrosym 2018”
Sarajevo: University of East Sarajevo, Faculty of Agriculture., 340.
https://hdl.handle.net/21.15107/rcub_ibiss_4349
Oljača J, Broćič Z, Pantelić D, Rudić J, Momčilović I. Prospective protein markers for selection of heat tolerant potato cultivars. in IX International Scientific Agriculture Symposium “Agrosym 2018”. 2018;:340.
https://hdl.handle.net/21.15107/rcub_ibiss_4349 .
Oljača, Jasmina, Broćič, Zoran, Pantelić, Danijel, Rudić, Jelena, Momčilović, Ivana, "Prospective protein markers for selection of heat tolerant potato cultivars" in IX International Scientific Agriculture Symposium “Agrosym 2018” (2018):340,
https://hdl.handle.net/21.15107/rcub_ibiss_4349 .

Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)

Savić, Jelena; Dragićević, Ivana; Pantelić, D.; Oljača, Jasmina; Momčilović, Ivana

(2012)

TY  - JOUR
AU  - Savić, Jelena
AU  - Dragićević, Ivana
AU  - Pantelić, D.
AU  - Oljača, Jasmina
AU  - Momčilović, Ivana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/298
AB  - We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.
T2  - Archives of Biological Sciences
T1  - Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)
IS  - 1
VL  - 64
SP  - 135
EP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_298
ER  - 
@article{
author = "Savić, Jelena and Dragićević, Ivana and Pantelić, D. and Oljača, Jasmina and Momčilović, Ivana",
year = "2012, 2012",
abstract = "We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.",
journal = "Archives of Biological Sciences",
title = "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)",
number = "1",
volume = "64",
pages = "135-144",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_298"
}
Savić, J., Dragićević, I., Pantelić, D., Oljača, J.,& Momčilović, I.. (2012). Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences, 64(1), 135-144.
https://hdl.handle.net/21.15107/rcub_ibiss_298
Savić J, Dragićević I, Pantelić D, Oljača J, Momčilović I. Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences. 2012;64(1):135-144.
https://hdl.handle.net/21.15107/rcub_ibiss_298 .
Savić, Jelena, Dragićević, Ivana, Pantelić, D., Oljača, Jasmina, Momčilović, Ivana, "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)" in Archives of Biological Sciences, 64, no. 1 (2012):135-144,
https://hdl.handle.net/21.15107/rcub_ibiss_298 .