TY  - JOUR
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Mirkov, Ivana
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Despotović, Marta
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5301
AB  - Objective The effect of oral cadmium (Cd) intake to influence contact skin allergies was examined,
since it is known that Cd is a heavy metal that affects many tissues, including the skin, in which it
disturbs homeostasis, thus resulting in inflammation and injury.
Methods Male rats were evoked with experimental contact hypersensitivity reaction (CHS) to hapten
dinitrochlorobenzene (DNCB), after prolonged (30 day) oral exposure to an environmentally relevant Cd
dose (5 ppm). The ear cell population was analyzed with flow cytometry. Cytokine production by ear
skin cells and the activity of skin-draining lymph node (DLN) cells were measured using enzyme-linked
immunosorbent assay (ELISA).
Results Orally acquired Cd (5 ppm) increased CHS intensity only in Dark Agouti (DA) rats by affecting
inflammatory responses in both the sensitization (an increase of IFN-γ and IL-17 cytokine production)
and challenge (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine production)
phases. An increased CHS reaction was seen in Albino Oxford (AO) rats only at a high Cd dose (50 ppm),
during the challenge phase (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine
production).
Conclusion These novel data indicate that oral Cd intensifies the skin response to sensitizing chemicals
such as DNCB.
PB  - Beijing: Chinese Center for Disease Control and Prevention
T2  - Biomedical and Environmental Sciences
T1  - Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats
IS  - 11
VL  - 35
DO  - 10.3967/bes2022.132
SP  - 1038
EP  - 1050
ER  - 

@article{
author = "Tucović, Dina and Kulaš, Jelena and Mirkov, Ivana and Popović, Dušanka and Zolotarevski, Lidija and Despotović, Marta and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2022",
abstract = "Objective The effect of oral cadmium (Cd) intake to influence contact skin allergies was examined,
since it is known that Cd is a heavy metal that affects many tissues, including the skin, in which it
disturbs homeostasis, thus resulting in inflammation and injury.
Methods Male rats were evoked with experimental contact hypersensitivity reaction (CHS) to hapten
dinitrochlorobenzene (DNCB), after prolonged (30 day) oral exposure to an environmentally relevant Cd
dose (5 ppm). The ear cell population was analyzed with flow cytometry. Cytokine production by ear
skin cells and the activity of skin-draining lymph node (DLN) cells were measured using enzyme-linked
immunosorbent assay (ELISA).
Results Orally acquired Cd (5 ppm) increased CHS intensity only in Dark Agouti (DA) rats by affecting
inflammatory responses in both the sensitization (an increase of IFN-γ and IL-17 cytokine production)
and challenge (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine production)
phases. An increased CHS reaction was seen in Albino Oxford (AO) rats only at a high Cd dose (50 ppm),
during the challenge phase (an increase of CD8+ and CD4+ cell number and TNF, IFN-γ and IL-17 cytokine
production).
Conclusion These novel data indicate that oral Cd intensifies the skin response to sensitizing chemicals
such as DNCB.",
publisher = "Beijing: Chinese Center for Disease Control and Prevention",
journal = "Biomedical and Environmental Sciences",
title = "Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats",
number = "11",
volume = "35",
doi = "10.3967/bes2022.132",
pages = "1038-1050"
}

Tucović, D., Kulaš, J., Mirkov, I., Popović, D., Zolotarevski, L., Despotović, M., Kataranovski, M.,& Popov Aleksandrov, A.. (2022). Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats. in Biomedical and Environmental Sciences
Beijing: Chinese Center for Disease Control and Prevention., 35(11), 1038-1050.
https://doi.org/10.3967/bes2022.132

Tucović D, Kulaš J, Mirkov I, Popović D, Zolotarevski L, Despotović M, Kataranovski M, Popov Aleksandrov A. Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats. in Biomedical and Environmental Sciences. 2022;35(11):1038-1050.
doi:10.3967/bes2022.132 .

Tucović, Dina, Kulaš, Jelena, Mirkov, Ivana, Popović, Dušanka, Zolotarevski, Lidija, Despotović, Marta, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Oral Cadmium Intake Enhances Contact Allergen-induced Skin Reaction in Rats" in Biomedical and Environmental Sciences, 35, no. 11 (2022):1038-1050,
https://doi.org/10.3967/bes2022.132 . .

TY  - CONF
AU  - Tucović, Dina
AU  - Mirkov, Ivana
AU  - Kulaš, Jelena
AU  - Popović, Dušanka
AU  - Zolotarevski, Lidija
AU  - Despotović, Marta
AU  - Kataranovski, Milena
AU  - Popov Aleksandrov, Aleksandra
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4882
AB  - Cadmium (Cd) in food and drinking water presents a health risk to the general population. We have shown previously that orally‐acquired Cd affects basal immune homeostasis in the skin. In this study, we examined the effect of 30‐days oral exposure of inflammatory disease‐prone Dark Agouti (DA) rats to two environmentally relevant Cd doses (5 and 50 ppm) on contact hypersensitivity reaction (CHS) induced by topical 0,4% dinitrochlorobenzene (DNCB). Both Cd doses increased proinflammatory epidermal cell response (IL‐1, TNF and IL‐6 production) to DNCB sensitization, as well as epidermal cells’ potential to stimulate naïve lymphocytes ex vivo (increased IFN‐γ and IL‐17 production in co‐cultures). The proinflammatory milieu of epidermal cells induced by sensitization was accompanied by increased hapten‐specific production of IFN‐γ (at a lower Cd dose) and IL‐17 (at both Cd doses) by draining lymph node (DLN) cells, compared to Cd non‐treated animals. During the challenge phase of CHS, oral Cd increased ear swelling response and skin inflammation (edema, mononuclear and neutrophil cell infiltration) at both Cd doses, what correlated with increased innate (TNF) and hapten‐specific effector (IFN‐γ, IL‐17) cytokine response by ear cells. Even in Albino Oxford (AO) rats generally less prone to inflammation, oral Cd increased the proinflammatory response of epidermal cells following sensitization, however, DLN cell responses were absent. Ear swelling response to hapten challenge was observed in AO individuals which acquired a higher Cd dose. Presented data imply the potential of food‐ and water‐borne Cd to be risk factors for skin disease development and/or its exacerbation.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Oral cadmium increases contact hypersensitivity reaction in rats
DO  - 10.1002/eji.202170200
SP  - 348
ER  - 

@conference{
author = "Tucović, Dina and Mirkov, Ivana and Kulaš, Jelena and Popović, Dušanka and Zolotarevski, Lidija and Despotović, Marta and Kataranovski, Milena and Popov Aleksandrov, Aleksandra",
year = "2021",
abstract = "Cadmium (Cd) in food and drinking water presents a health risk to the general population. We have shown previously that orally‐acquired Cd affects basal immune homeostasis in the skin. In this study, we examined the effect of 30‐days oral exposure of inflammatory disease‐prone Dark Agouti (DA) rats to two environmentally relevant Cd doses (5 and 50 ppm) on contact hypersensitivity reaction (CHS) induced by topical 0,4% dinitrochlorobenzene (DNCB). Both Cd doses increased proinflammatory epidermal cell response (IL‐1, TNF and IL‐6 production) to DNCB sensitization, as well as epidermal cells’ potential to stimulate naïve lymphocytes ex vivo (increased IFN‐γ and IL‐17 production in co‐cultures). The proinflammatory milieu of epidermal cells induced by sensitization was accompanied by increased hapten‐specific production of IFN‐γ (at a lower Cd dose) and IL‐17 (at both Cd doses) by draining lymph node (DLN) cells, compared to Cd non‐treated animals. During the challenge phase of CHS, oral Cd increased ear swelling response and skin inflammation (edema, mononuclear and neutrophil cell infiltration) at both Cd doses, what correlated with increased innate (TNF) and hapten‐specific effector (IFN‐γ, IL‐17) cytokine response by ear cells. Even in Albino Oxford (AO) rats generally less prone to inflammation, oral Cd increased the proinflammatory response of epidermal cells following sensitization, however, DLN cell responses were absent. Ear swelling response to hapten challenge was observed in AO individuals which acquired a higher Cd dose. Presented data imply the potential of food‐ and water‐borne Cd to be risk factors for skin disease development and/or its exacerbation.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Oral cadmium increases contact hypersensitivity reaction in rats",
doi = "10.1002/eji.202170200",
pages = "348"
}

Tucović, D., Mirkov, I., Kulaš, J., Popović, D., Zolotarevski, L., Despotović, M., Kataranovski, M.,& Popov Aleksandrov, A.. (2021). Oral cadmium increases contact hypersensitivity reaction in rats. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 348.
https://doi.org/10.1002/eji.202170200

Tucović D, Mirkov I, Kulaš J, Popović D, Zolotarevski L, Despotović M, Kataranovski M, Popov Aleksandrov A. Oral cadmium increases contact hypersensitivity reaction in rats. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;:348.
doi:10.1002/eji.202170200 .

Tucović, Dina, Mirkov, Ivana, Kulaš, Jelena, Popović, Dušanka, Zolotarevski, Lidija, Despotović, Marta, Kataranovski, Milena, Popov Aleksandrov, Aleksandra, "Oral cadmium increases contact hypersensitivity reaction in rats" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting (2021):348,
https://doi.org/10.1002/eji.202170200 . .