TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6693
AB  - Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.
PB  - Belgrade: Serbian Society for Microbiology
C3  - Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
T1  - Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6693
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2024",
abstract = "Pollution of surface waters still represents one of the major environmental challenges in the Republic of Serbia, where wastewaters are discharged directly into the recipients without proper treatment. Within the last decade, we have assessed the microbiological water quality of more than 100 sites situated at different water bodies in Serbia. Quality was determined using faecal indicator bacteria, while microbial source tracking was employed to assess the source of pollution. Almost 50 % of the investigated sites were characterized by critical or even higher level of faecal contamination indicating that untreated wastewaters indeed represent significant pollution pressure on surface waters. Human-associated markers were prevalent in samples from the majority of contaminated sites but the source of pollution was not exclusively human-associated. 
The impact on the water quality of the Danube River was demonstrated in our previous research conducted at the whole river level within the Joint Danube Surveys. In the river stretch from Novi Sad to its confluence with the Velika Morava River, all the midstream samples were critically polluted. In this section, the highest level of pollution was recorded downstream of Belgrade. As ultimate recipients of wastewaters, Danube and its largest tributary Sava currently represent the only solution for disposing of wastewaters originating from the Serbian capital‘s 1,700,000 inhabitants. Such kind of disposal rises additional issues such as antimicrobial resistance and presence of infectious agents in water. For instance during COVID-19 pandemic, we have demonstrated that SARS-CoV-2 RNA can be detected even in surface waters of the Danube River at the sites receiving high wastewater loads from Belgrade which was the unique case for the Basin. 
Despites its extreme importance in this case, wastewater-based epidemiology is neglected in our country and hereby we would like to emphasize the need for implementation of program of such kind in Serbia.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia",
title = "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia",
pages = "65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6693"
}

Kolarević, S., Micsinai, A., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2024). Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia
Belgrade: Serbian Society for Microbiology., 65.
https://hdl.handle.net/21.15107/rcub_ibiss_6693

Kolarević S, Micsinai A, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia. in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia. 2024;:65.
https://hdl.handle.net/21.15107/rcub_ibiss_6693 .

Kolarević, Stoimir, Micsinai, Adrienn, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Impact of untreated wastewaters on the microbiological water quality  of the Danube River  and its tributaries in Serbia" in Book of abstracts: 13th Congress of Microbiologists of Serbia: Mikromed Regio 5: From biotechnology to human and planetary health; 2024 Apr 4-6; Belgrade, Serbia (2024):65,
https://hdl.handle.net/21.15107/rcub_ibiss_6693 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Adrienn, Micsinai
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević, Ana
AU  - Vojnović-Milutinović, Danijela
AU  - Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukić, Aleksandar
AU  - Kostić, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6591
AB  - Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6591
ER  - 

@conference{
author = "Kolarević, Stoimir and Adrienn, Micsinai and Szanto-Egesz, Reka and Lukacs, Alena and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević, Ana and Vojnović-Milutinović, Danijela and Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukić, Aleksandar and Kostić, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2023",
abstract = "Pollution of natural water bodies is one of the biggest challenges in developing countries, such as
Serbia, where wastewater is discharged directly into the recipients without proper treatment and
poses serious threats to surface water quality, general safety and environmental health. Numerous
pathogenic microorganisms, bacteria, viruses, protozoa and helminthes may reach surface waters
and eventually contaminate groundwater. Despites its extreme importance in this case,
implementation of wastewater-based epidemiology is a challenge for developing countries, as the
majority of households are not connected to sewerage systems. Within our research, we have placed
major focus on recipients of wastewaters as an alternative of targeting raw wastewater. During the
fourth COVID-19 wave in Serbia that started in late February 2021 multiple water samples were
collected at 12 sites at Sava and Danube Rivers in the Belgrade city area which are under the impact
of untreated wastewaters.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2)
and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial
faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological
parameters were analysed in parallel. Out of 44 samples analyzed, 31 were positive for at least one
of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in
surface waters in context with the large amount of epidemiological and environmental metadata can
be used as epidemiological early-warning tool in countries with poor wastewater treatment.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia",
pages = "11",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6591"
}

Kolarević, S., Adrienn, M., Szanto-Egesz, R., Lukacs, A., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević, A., Vojnović-Milutinović, D., Kirschner, A., Farnleitner, A., Linke, R., Đukić, A., Kostić, J., Sunjog, K.,& Paunović, M.. (2023). Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591

Kolarević S, Adrienn M, Szanto-Egesz R, Lukacs A, Kračun-Kolarević M, Jovanović Marić J, Đorđević A, Vojnović-Milutinović D, Kirschner A, Farnleitner A, Linke R, Đukić A, Kostić J, Sunjog K, Paunović M. Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:11.
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .

Kolarević, Stoimir, Adrienn, Micsinai, Szanto-Egesz, Reka, Lukacs, Alena, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević, Ana, Vojnović-Milutinović, Danijela, Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukić, Aleksandar, Kostić, Jovana, Sunjog, Karolina, Paunović, Momir, "Applicability of wastewater based epidemiology in countries with poor wastewater treatment – COVID-19 case study in Serbia" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):11,
https://hdl.handle.net/21.15107/rcub_ibiss_6591 .