TY  - JOUR
AU  - Dakić, Tamara B.
AU  - Jevđović, Tanja V.
AU  - Perić, Mina I.
AU  - Bjelobaba, Ivana
AU  - Markelić, Milica B.
AU  - Milutinović, Bojana S.
AU  - Lakić, Iva V.
AU  - Jasnić, Nebojša I.
AU  - Đorđević, Jelena D.
AU  - Vujović, Predrag Z.
PY  - 2017
UR  - http://doi.wiley.com/10.1111/ejn.13607
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2779
AB  - In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of fasting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular.
T2  - European Journal of Neuroscience
T1  - Short-term fasting promotes insulin expression in rat hypothalamus
IS  - 1
VL  - 46
DO  - 10.1111/ejn.13607
SP  - 1730
EP  - 1737
ER  - 

@article{
author = "Dakić, Tamara B. and Jevđović, Tanja V. and Perić, Mina I. and Bjelobaba, Ivana and Markelić, Milica B. and Milutinović, Bojana S. and Lakić, Iva V. and Jasnić, Nebojša I. and Đorđević, Jelena D. and Vujović, Predrag Z.",
year = "2017",
abstract = "In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of fasting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular.",
journal = "European Journal of Neuroscience",
title = "Short-term fasting promotes insulin expression in rat hypothalamus",
number = "1",
volume = "46",
doi = "10.1111/ejn.13607",
pages = "1730-1737"
}

Dakić, T. B., Jevđović, T. V., Perić, M. I., Bjelobaba, I., Markelić, M. B., Milutinović, B. S., Lakić, I. V., Jasnić, N. I., Đorđević, J. D.,& Vujović, P. Z.. (2017). Short-term fasting promotes insulin expression in rat hypothalamus. in European Journal of Neuroscience, 46(1), 1730-1737.
https://doi.org/10.1111/ejn.13607

Dakić TB, Jevđović TV, Perić MI, Bjelobaba I, Markelić MB, Milutinović BS, Lakić IV, Jasnić NI, Đorđević JD, Vujović PZ. Short-term fasting promotes insulin expression in rat hypothalamus. in European Journal of Neuroscience. 2017;46(1):1730-1737.
doi:10.1111/ejn.13607 .

Dakić, Tamara B., Jevđović, Tanja V., Perić, Mina I., Bjelobaba, Ivana, Markelić, Milica B., Milutinović, Bojana S., Lakić, Iva V., Jasnić, Nebojša I., Đorđević, Jelena D., Vujović, Predrag Z., "Short-term fasting promotes insulin expression in rat hypothalamus" in European Journal of Neuroscience, 46, no. 1 (2017):1730-1737,
https://doi.org/10.1111/ejn.13607 . .