TY  - JOUR
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nikolić, Tijana
AU  - Novaković, Lazar
AU  - Lukić, Milana
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Filipović, Biljana
AU  - Mišić, Danijela
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0031942218303777?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3146
AB  - While bioactive properties of Centaurium erythraea Rafn secoiridoid glucosides (SG) are widely recognized, many aspects related to their biochemistry, metabolism and relationship to the overall plant physiology are not yet understood. Here we present for the first time an insight into the molecular background of organ-specific and genotype-dependent constitutive biosynthesis of secoiridoids in C. erythraea, by comparing chemical profiles and secoiridoid glucosides-related gene expression. Genes encoding enzymes for intermediate steps of secoiridoids biosynthesis up to secologanin have been identified by analysing transcriptomic data from C. erythraea leaves. Results suggest an organ-specific capacity for the production and accumulation of secoiridoid glucosides, and highlight leaves as the main biosynthesis site. They also point out that significant differences in SG content among various C. erythraea genotypes, are, at least partially, determined by different expression patterns of SG-related genes. The biosynthesis of SG in C. erythraea leaves is enhanced upon treatments with methyl jasmonate (MeJA), which causes reprogramming of SG-related gene expression, leading to an increased production of valuable bioactive compounds. The present study unveiled several rate-limiting genes (encoding GES, G8O, 8HGO, IS and 7DLGT) in SG biosynthesis. SLS and CPR are highlighted as important genes/enzymes that might regulate biosynthetic flux through SG pathway. Information gathered within this study will help us gain deeper insight into the SG metabolism and develop strategies for enhanced biosynthesis of specific secoiridoid glucosides in homologous or heterologous systems.
T2  - Phytochemistry
T1  - Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.
VL  - 155
DO  - 10.1016/j.phytochem.2018.07.015
SP  - 69
EP  - 82
ER  - 

@article{
author = "Matekalo, Dragana and Skorić, Marijana and Nikolić, Tijana and Novaković, Lazar and Lukić, Milana and Božunović, Jelena and Aničić, Neda and Filipović, Biljana and Mišić, Danijela",
year = "2018",
abstract = "While bioactive properties of Centaurium erythraea Rafn secoiridoid glucosides (SG) are widely recognized, many aspects related to their biochemistry, metabolism and relationship to the overall plant physiology are not yet understood. Here we present for the first time an insight into the molecular background of organ-specific and genotype-dependent constitutive biosynthesis of secoiridoids in C. erythraea, by comparing chemical profiles and secoiridoid glucosides-related gene expression. Genes encoding enzymes for intermediate steps of secoiridoids biosynthesis up to secologanin have been identified by analysing transcriptomic data from C. erythraea leaves. Results suggest an organ-specific capacity for the production and accumulation of secoiridoid glucosides, and highlight leaves as the main biosynthesis site. They also point out that significant differences in SG content among various C. erythraea genotypes, are, at least partially, determined by different expression patterns of SG-related genes. The biosynthesis of SG in C. erythraea leaves is enhanced upon treatments with methyl jasmonate (MeJA), which causes reprogramming of SG-related gene expression, leading to an increased production of valuable bioactive compounds. The present study unveiled several rate-limiting genes (encoding GES, G8O, 8HGO, IS and 7DLGT) in SG biosynthesis. SLS and CPR are highlighted as important genes/enzymes that might regulate biosynthetic flux through SG pathway. Information gathered within this study will help us gain deeper insight into the SG metabolism and develop strategies for enhanced biosynthesis of specific secoiridoid glucosides in homologous or heterologous systems.",
journal = "Phytochemistry",
title = "Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.",
volume = "155",
doi = "10.1016/j.phytochem.2018.07.015",
pages = "69-82"
}

Matekalo, D., Skorić, M., Nikolić, T., Novaković, L., Lukić, M., Božunović, J., Aničić, N., Filipović, B.,& Mišić, D.. (2018). Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.. in Phytochemistry, 155, 69-82.
https://doi.org/10.1016/j.phytochem.2018.07.015

Matekalo D, Skorić M, Nikolić T, Novaković L, Lukić M, Božunović J, Aničić N, Filipović B, Mišić D. Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.. in Phytochemistry. 2018;155:69-82.
doi:10.1016/j.phytochem.2018.07.015 .

Matekalo, Dragana, Skorić, Marijana, Nikolić, Tijana, Novaković, Lazar, Lukić, Milana, Božunović, Jelena, Aničić, Neda, Filipović, Biljana, Mišić, Danijela, "Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate." in Phytochemistry, 155 (2018):69-82,
https://doi.org/10.1016/j.phytochem.2018.07.015 . .

TY  - CONF
AU  - Nikolić, Tijana
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Novaković, Lazar
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Mišić, Danijela
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4291
AB  - Plant species Centaurium erythraea Rafn (fam. Gentianaceae) is characterized by the presence
of secoiridoid glucosides (SGs) as dominant secondary metabolites. The SG biosynthetic pathway
has not yet been fully elucidated, despite the great pharmacological importance of this species.
Here, an insight into the SG biosynthesis is achieved by comparing chemical profiles and secoiridoid-
related gene expression patterns of different C. erythraea genotypes and plant organs. The
results revealed that leaves are the main site of secoiridoid biosynthesis and accumulation in C.
erythraea. The key function in the secoiridoid glucoside biosynthetic pathway has been assigned
to genes encoding GES, G8O, 8HGO, 7DLGT and 7DLH2, while for SLS and CPR a potential biosynthetic-
flux regulatory role has been determined. The correlation between the levels of these
genes’ expression and SG content is evident in different plant organs. Also, the analysis of SG
high- and low-productive genotypes of C. erythraea points out that chemical variability existing at
intra-species level is, at least partially, determined by the different patterns of expression of SG-related
genes in different genotypes. Taking into consideration the biological activity of secoiridoid
glucosides, not only is the information obtained in this study of importance for further SG biosynthesis
elucidation, but it also shows a great potential for future biotechnology-based sustainable
production of these valuable metabolites.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts
T1  - Organ-specificity and genotype-dependency of secoiridoid glucosides’ constitutive biosynthesis in Centaurium erythraea Rafn
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4291
ER  - 

@conference{
author = "Nikolić, Tijana and Matekalo, Dragana and Skorić, Marijana and Novaković, Lazar and Božunović, Jelena and Aničić, Neda and Mišić, Danijela",
year = "2018",
abstract = "Plant species Centaurium erythraea Rafn (fam. Gentianaceae) is characterized by the presence
of secoiridoid glucosides (SGs) as dominant secondary metabolites. The SG biosynthetic pathway
has not yet been fully elucidated, despite the great pharmacological importance of this species.
Here, an insight into the SG biosynthesis is achieved by comparing chemical profiles and secoiridoid-
related gene expression patterns of different C. erythraea genotypes and plant organs. The
results revealed that leaves are the main site of secoiridoid biosynthesis and accumulation in C.
erythraea. The key function in the secoiridoid glucoside biosynthetic pathway has been assigned
to genes encoding GES, G8O, 8HGO, 7DLGT and 7DLH2, while for SLS and CPR a potential biosynthetic-
flux regulatory role has been determined. The correlation between the levels of these
genes’ expression and SG content is evident in different plant organs. Also, the analysis of SG
high- and low-productive genotypes of C. erythraea points out that chemical variability existing at
intra-species level is, at least partially, determined by the different patterns of expression of SG-related
genes in different genotypes. Taking into consideration the biological activity of secoiridoid
glucosides, not only is the information obtained in this study of importance for further SG biosynthesis
elucidation, but it also shows a great potential for future biotechnology-based sustainable
production of these valuable metabolites.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts",
title = "Organ-specificity and genotype-dependency of secoiridoid glucosides’ constitutive biosynthesis in Centaurium erythraea Rafn",
pages = "126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4291"
}

Nikolić, T., Matekalo, D., Skorić, M., Novaković, L., Božunović, J., Aničić, N.,& Mišić, D.. (2018). Organ-specificity and genotype-dependency of secoiridoid glucosides’ constitutive biosynthesis in Centaurium erythraea Rafn. in Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts
Belgrade: Serbian Plant Physiology Society., 126.
https://hdl.handle.net/21.15107/rcub_ibiss_4291

Nikolić T, Matekalo D, Skorić M, Novaković L, Božunović J, Aničić N, Mišić D. Organ-specificity and genotype-dependency of secoiridoid glucosides’ constitutive biosynthesis in Centaurium erythraea Rafn. in Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts. 2018;:126.
https://hdl.handle.net/21.15107/rcub_ibiss_4291 .

Nikolić, Tijana, Matekalo, Dragana, Skorić, Marijana, Novaković, Lazar, Božunović, Jelena, Aničić, Neda, Mišić, Danijela, "Organ-specificity and genotype-dependency of secoiridoid glucosides’ constitutive biosynthesis in Centaurium erythraea Rafn" in Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts (2018):126,
https://hdl.handle.net/21.15107/rcub_ibiss_4291 .

TY  - CONF
AU  - Novaković, Lazar
AU  - Živković, Suzana
AU  - Popović-Bijelić, Ana
AU  - Mišić, Danijela
AU  - Mojović, Miloš
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6189
AB  - Electron paramagnetic resonance spectroscopy (EPR) was applied for in vivo detection of oxidative stress induced by high ionic strengths in Centaurium erythraea Rafn (Gentianaceae), a herbaceous perennial often found on saline soils. Shoots of C. erythraea were cultured in vitro for 4 weeks on solid 1/2 MS medium, or 1/2 MS medium supplemented with 200 mM NaCl to induce salt stress and thus stimulate the production of reactive oxygen species (ROS), which is a common response of plant cells subjected to various types of biotic or abiotic stresses. The reduction of a stable cell-permeable aminoxyl radical spin probe, Tempo, was measured to assess the oxidative status of the control and salt-treated samples. In both types of samples, the reduction of Tempo showed zero-order kinetics. After one hour, the control reduced only 9% of the initial amount of the spin probe, whereas the sample grown with 200 mM NaCl reduced it by 20%. This may indicate that the plants grown in the presence of 200 mM NaCl produced higher amounts of ROS which are able to reduce the aminoxyl radical. The obtained results indicate that EPR can be used as a method for in vivo evaluation of the redox state of plants under stress conditions, and could also be useful in determining the salt-stress tolerance of plants. Further
studies, including different salt concentrations, and plant species, have to be conducted to verify these findings.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn
SP  - 178
EP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6189
ER  - 

@conference{
author = "Novaković, Lazar and Živković, Suzana and Popović-Bijelić, Ana and Mišić, Danijela and Mojović, Miloš",
year = "2015",
abstract = "Electron paramagnetic resonance spectroscopy (EPR) was applied for in vivo detection of oxidative stress induced by high ionic strengths in Centaurium erythraea Rafn (Gentianaceae), a herbaceous perennial often found on saline soils. Shoots of C. erythraea were cultured in vitro for 4 weeks on solid 1/2 MS medium, or 1/2 MS medium supplemented with 200 mM NaCl to induce salt stress and thus stimulate the production of reactive oxygen species (ROS), which is a common response of plant cells subjected to various types of biotic or abiotic stresses. The reduction of a stable cell-permeable aminoxyl radical spin probe, Tempo, was measured to assess the oxidative status of the control and salt-treated samples. In both types of samples, the reduction of Tempo showed zero-order kinetics. After one hour, the control reduced only 9% of the initial amount of the spin probe, whereas the sample grown with 200 mM NaCl reduced it by 20%. This may indicate that the plants grown in the presence of 200 mM NaCl produced higher amounts of ROS which are able to reduce the aminoxyl radical. The obtained results indicate that EPR can be used as a method for in vivo evaluation of the redox state of plants under stress conditions, and could also be useful in determining the salt-stress tolerance of plants. Further
studies, including different salt concentrations, and plant species, have to be conducted to verify these findings.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn",
pages = "178-179",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6189"
}

Novaković, L., Živković, S., Popović-Bijelić, A., Mišić, D.,& Mojović, M.. (2015). Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 178-179.
https://hdl.handle.net/21.15107/rcub_ibiss_6189

Novaković L, Živković S, Popović-Bijelić A, Mišić D, Mojović M. Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:178-179.
https://hdl.handle.net/21.15107/rcub_ibiss_6189 .

Novaković, Lazar, Živković, Suzana, Popović-Bijelić, Ana, Mišić, Danijela, Mojović, Miloš, "Application of Tempo EPR spin probe for in vivo detection of salt-induced oxidative stress in Centaurium erythraea Rafn" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):178-179,
https://hdl.handle.net/21.15107/rcub_ibiss_6189 .

TY  - CONF
AU  - Nikolovski, Neda
AU  - Novaković, Lazar
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Avramov, Stevan
AU  - Skorić, Marijana
AU  - Mišić, Danijela
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6814
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade
T1  - Employment of DNA markers and morphological traits to estimate genetic variability of the extremly rare Centaurium littorale ssp. uliginosum in Serbia
SP  - 150
EP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6814
ER  - 

@conference{
author = "Nikolovski, Neda and Novaković, Lazar and Šiler, Branislav and Banjanac, Tijana and Avramov, Stevan and Skorić, Marijana and Mišić, Danijela",
year = "2013",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade",
title = "Employment of DNA markers and morphological traits to estimate genetic variability of the extremly rare Centaurium littorale ssp. uliginosum in Serbia",
pages = "150-151",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6814"
}

Nikolovski, N., Novaković, L., Šiler, B., Banjanac, T., Avramov, S., Skorić, M.,& Mišić, D.. (2013). Employment of DNA markers and morphological traits to estimate genetic variability of the extremly rare Centaurium littorale ssp. uliginosum in Serbia. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade
Belgrade: Serbian Plant Physiology Society., 150-151.
https://hdl.handle.net/21.15107/rcub_ibiss_6814

Nikolovski N, Novaković L, Šiler B, Banjanac T, Avramov S, Skorić M, Mišić D. Employment of DNA markers and morphological traits to estimate genetic variability of the extremly rare Centaurium littorale ssp. uliginosum in Serbia. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade. 2013;:150-151.
https://hdl.handle.net/21.15107/rcub_ibiss_6814 .

Nikolovski, Neda, Novaković, Lazar, Šiler, Branislav, Banjanac, Tijana, Avramov, Stevan, Skorić, Marijana, Mišić, Danijela, "Employment of DNA markers and morphological traits to estimate genetic variability of the extremly rare Centaurium littorale ssp. uliginosum in Serbia" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade (2013):150-151,
https://hdl.handle.net/21.15107/rcub_ibiss_6814 .

TY  - CONF
AU  - Novaković, Lazar
AU  - Nikolovski, Neda
AU  - Šiler, Branislav
AU  - Banjanac, Tijana
AU  - Ghalawenji, Nabil
AU  - Skorić, Marijana
AU  - Mišić, Danijela
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6816
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade
T1  - Assessment of genetic diversity and population structure of Blackstonia perfoliata (L.) Huds. From the central Balkans
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6816
ER  - 

@conference{
author = "Novaković, Lazar and Nikolovski, Neda and Šiler, Branislav and Banjanac, Tijana and Ghalawenji, Nabil and Skorić, Marijana and Mišić, Danijela",
year = "2013",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade",
title = "Assessment of genetic diversity and population structure of Blackstonia perfoliata (L.) Huds. From the central Balkans",
pages = "150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6816"
}

Novaković, L., Nikolovski, N., Šiler, B., Banjanac, T., Ghalawenji, N., Skorić, M.,& Mišić, D.. (2013). Assessment of genetic diversity and population structure of Blackstonia perfoliata (L.) Huds. From the central Balkans. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade
Belgrade: Serbian Plant Physiology Society., 150.
https://hdl.handle.net/21.15107/rcub_ibiss_6816

Novaković L, Nikolovski N, Šiler B, Banjanac T, Ghalawenji N, Skorić M, Mišić D. Assessment of genetic diversity and population structure of Blackstonia perfoliata (L.) Huds. From the central Balkans. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade. 2013;:150.
https://hdl.handle.net/21.15107/rcub_ibiss_6816 .

Novaković, Lazar, Nikolovski, Neda, Šiler, Branislav, Banjanac, Tijana, Ghalawenji, Nabil, Skorić, Marijana, Mišić, Danijela, "Assessment of genetic diversity and population structure of Blackstonia perfoliata (L.) Huds. From the central Balkans" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Belgrade (2013):150,
https://hdl.handle.net/21.15107/rcub_ibiss_6816 .