TY  - JOUR
AU  - Bukara, Katarina
AU  - Drvenica, Ivana
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Mišić, Danijela
AU  - Vasić, Borislav
AU  - Gajić, Radoš
AU  - Vučetić, Dušan
AU  - Kiekens, Filip
AU  - Bugarski, Branko
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0168165616315735
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84992192143&origin=SingleRecordEmailAlert&txGid=694651161B2E63C5600F6DE538B0ACEC.wsnAw8kcdt7IPYLO0V48gA:3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2525
AB  - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials – porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process – gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
T2  - Journal of Biotechnology
T1  - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
VL  - 240
DO  - 10.1016/j.jbiotec.2016.10.017
SP  - 14
EP  - 22
ER  - 

@article{
author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna and Stančić, Ana and Mišić, Danijela and Vasić, Borislav and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko",
year = "2016",
abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials – porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process – gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.",
journal = "Journal of Biotechnology",
title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts",
volume = "240",
doi = "10.1016/j.jbiotec.2016.10.017",
pages = "14-22"
}

Bukara, K., Drvenica, I., Ilić, V., Stančić, A., Mišić, D., Vasić, B., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology, 240, 14-22.
https://doi.org/10.1016/j.jbiotec.2016.10.017

Bukara K, Drvenica I, Ilić V, Stančić A, Mišić D, Vasić B, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22.
doi:10.1016/j.jbiotec.2016.10.017 .

Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna, Stančić, Ana, Mišić, Danijela, Vasić, Borislav, Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22,
https://doi.org/10.1016/j.jbiotec.2016.10.017 . .

TY  - JOUR
AU  - Borovčanin, Nemanja
AU  - Vučetić, Dušan
AU  - Stamenković, Gorana
AU  - Jocić, Miodrag
AU  - Jovičić, Dragana
AU  - Balint, Bela
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/48
AB  - Nucleic-acid Amplification Testing (NAT) consist of extraction, amplification, hybridization and detection. Polymerase Chain Reaction (PCR) is method for amplification of viral genetic material (DNA/RNA) in enough number of copies, so we can detect them. Since year 1943, when transmission of hepatitis via transfusion was reported till beginning of 21st century, when NAT was implemented, large number of tests for detection of hepatitis B and C virus (HBV, HCV) and human immunodeficiency virus (HIV) were developed. In this article, we describe basic princples of detection of these viruses by NAT. We also show results from testing samples of blood donors in Institute of Transfusiology from April 2007 till June 2011. From 50 369 donors testing in Mini Pools (MP) of 24, we found two HCV RNA positive samples, one HBV DNA positive sample and all pools were HIV RNA negative. From 2 689 samples which were testing by Individual Donation (ID) NAT, 135 were HBV DNA positive, 108 were HCV RNA positive and 7 were HIV RNA positive. All samples also were tested by Enzyme Link Immunosorbent Assay (ELISA) and Confirmatory tests. Comparison of our results show validity of NAT testing, especially when ELISA tests of less specificity and sensitivity were used.
AB  - Da bi testiranje nukleinskih kiselina bilo moguće, neophodno je izdvojiti ih iz virusa (ekstrakcija), zatim umnožiti (amplifikacija), obeležiti specifičnim probama (hibridizacija) i detektovati. To se obavlja korišćenjem metode 'Polymerase Chain Reaction' (PCR) ili lančane reakcije polimeraze koja nam omogućava da umnožimo deo virusnog genoma do količine koju je moguće detektovati. Od 1943. godine, kada je transmisija hepatitisa preko transfuzije prvi put prijavljena, do početka 21. veka, kada je masovno uvedena NAT tehnologija (eng. Nucleic-acid Amplification Testing - testiranje amplifikovanih nukleinskih kiselina), došlo je do značajnog poboljšanja u otkrivanju virusa uzročnika hematogenih transmisivnih bolesti. U ovom radu dati su osnovni principi NAT-a, odnosno detekcije genetskog materijala virusa uzročnika hepatitisa tipa B, C i virusa humane imunodeficijencije (HIV-a). Takođe, opisani su NAT rezultati dobijeni kod uzoraka krvi davalaca u Institutu za transfuziologiju VMA u periodu od aprila 2007. do juna 2011. godine. Od 50.369 testiranih davalaca u pulu, detektovana su dva HCV RNK pozitivna uzorka, jedan HBV DNK pozitivan uzorak, a za HIV RNK svi pulovi su bili negativni. Od 2.689 pojedinačno obavljenih NAT testiranja, najviše pozitivnih bilo je za HBV DNK (135 uzoraka), zatim za HCV RNK (108 pozitivnih) i 7 pozitivnih za HIV RNK. Za sve navedene uzorke paralelno su rađeni određeni enzimoimunski i potvrdni testovi. Komparacija rezultata primenjenih testova na našim ispitanim uzorcima potvrdila je opravdanost primene NAT-a, posebno prilikom korišćenja manje specifičnih i osetljivih enzimoimunskih testova.
T2  - Bilten za transfuziologiju
T1  - Principi NAT tehnologije sa osvrtom na rezultate Instituta za transfuziologiju VMA u periodu od 2007. do 2011. godine
T1  - Principles of NAT technology in view of the results obtained at the Institute of Transfusiology of MMA from 2007 till 2011
IS  - 1-2
VL  - 57
SP  - 99
EP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_48
ER  - 

@article{
author = "Borovčanin, Nemanja and Vučetić, Dušan and Stamenković, Gorana and Jocić, Miodrag and Jovičić, Dragana and Balint, Bela",
year = "2011, 2011",
abstract = "Nucleic-acid Amplification Testing (NAT) consist of extraction, amplification, hybridization and detection. Polymerase Chain Reaction (PCR) is method for amplification of viral genetic material (DNA/RNA) in enough number of copies, so we can detect them. Since year 1943, when transmission of hepatitis via transfusion was reported till beginning of 21st century, when NAT was implemented, large number of tests for detection of hepatitis B and C virus (HBV, HCV) and human immunodeficiency virus (HIV) were developed. In this article, we describe basic princples of detection of these viruses by NAT. We also show results from testing samples of blood donors in Institute of Transfusiology from April 2007 till June 2011. From 50 369 donors testing in Mini Pools (MP) of 24, we found two HCV RNA positive samples, one HBV DNA positive sample and all pools were HIV RNA negative. From 2 689 samples which were testing by Individual Donation (ID) NAT, 135 were HBV DNA positive, 108 were HCV RNA positive and 7 were HIV RNA positive. All samples also were tested by Enzyme Link Immunosorbent Assay (ELISA) and Confirmatory tests. Comparison of our results show validity of NAT testing, especially when ELISA tests of less specificity and sensitivity were used., Da bi testiranje nukleinskih kiselina bilo moguće, neophodno je izdvojiti ih iz virusa (ekstrakcija), zatim umnožiti (amplifikacija), obeležiti specifičnim probama (hibridizacija) i detektovati. To se obavlja korišćenjem metode 'Polymerase Chain Reaction' (PCR) ili lančane reakcije polimeraze koja nam omogućava da umnožimo deo virusnog genoma do količine koju je moguće detektovati. Od 1943. godine, kada je transmisija hepatitisa preko transfuzije prvi put prijavljena, do početka 21. veka, kada je masovno uvedena NAT tehnologija (eng. Nucleic-acid Amplification Testing - testiranje amplifikovanih nukleinskih kiselina), došlo je do značajnog poboljšanja u otkrivanju virusa uzročnika hematogenih transmisivnih bolesti. U ovom radu dati su osnovni principi NAT-a, odnosno detekcije genetskog materijala virusa uzročnika hepatitisa tipa B, C i virusa humane imunodeficijencije (HIV-a). Takođe, opisani su NAT rezultati dobijeni kod uzoraka krvi davalaca u Institutu za transfuziologiju VMA u periodu od aprila 2007. do juna 2011. godine. Od 50.369 testiranih davalaca u pulu, detektovana su dva HCV RNK pozitivna uzorka, jedan HBV DNK pozitivan uzorak, a za HIV RNK svi pulovi su bili negativni. Od 2.689 pojedinačno obavljenih NAT testiranja, najviše pozitivnih bilo je za HBV DNK (135 uzoraka), zatim za HCV RNK (108 pozitivnih) i 7 pozitivnih za HIV RNK. Za sve navedene uzorke paralelno su rađeni određeni enzimoimunski i potvrdni testovi. Komparacija rezultata primenjenih testova na našim ispitanim uzorcima potvrdila je opravdanost primene NAT-a, posebno prilikom korišćenja manje specifičnih i osetljivih enzimoimunskih testova.",
journal = "Bilten za transfuziologiju",
title = "Principi NAT tehnologije sa osvrtom na rezultate Instituta za transfuziologiju VMA u periodu od 2007. do 2011. godine, Principles of NAT technology in view of the results obtained at the Institute of Transfusiology of MMA from 2007 till 2011",
number = "1-2",
volume = "57",
pages = "99-107",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_48"
}

Borovčanin, N., Vučetić, D., Stamenković, G., Jocić, M., Jovičić, D.,& Balint, B.. (2011). Principi NAT tehnologije sa osvrtom na rezultate Instituta za transfuziologiju VMA u periodu od 2007. do 2011. godine. in Bilten za transfuziologiju, 57(1-2), 99-107.
https://hdl.handle.net/21.15107/rcub_ibiss_48

Borovčanin N, Vučetić D, Stamenković G, Jocić M, Jovičić D, Balint B. Principi NAT tehnologije sa osvrtom na rezultate Instituta za transfuziologiju VMA u periodu od 2007. do 2011. godine. in Bilten za transfuziologiju. 2011;57(1-2):99-107.
https://hdl.handle.net/21.15107/rcub_ibiss_48 .

Borovčanin, Nemanja, Vučetić, Dušan, Stamenković, Gorana, Jocić, Miodrag, Jovičić, Dragana, Balint, Bela, "Principi NAT tehnologije sa osvrtom na rezultate Instituta za transfuziologiju VMA u periodu od 2007. do 2011. godine" in Bilten za transfuziologiju, 57, no. 1-2 (2011):99-107,
https://hdl.handle.net/21.15107/rcub_ibiss_48 .