TY  - JOUR
AU  - Ilić, Marija
AU  - Stamenković, Gorana
AU  - Nikolić, Valentina
AU  - Marković, Vanja
AU  - Marinković, Nikola
AU  - Paunović, Momir
AU  - Crnobrnja-Isailović, Jelka
PY  - 2016
UR  - http://epa.oszk.hu/02500/02583/00043/pdf/EPA02583_applied_ecology_2016_02_381397.pdf
UR  - http://www.aloki.hu/pdf/1402_381397.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2596
AB  - Many European frogs and toads are relatively secretive species and except during breeding season, adults can rarely be seen during time-restricted fieldwork. In contrast, their tadpoles are easy to record and could be very useful in a brief biodiversity assessment. It is important to perform quick and accurate taxonomic identification of tadpoles, yet genetic methods are costly and cannot be routinely applied. We tested suitability of morphometric analysis for taxonomical distinction among tadpoles of early breeding local anuran species. Tadpole samples were collected simultaneously at three different locations in Republic of Serbia (South-eastern Europe) in habitats known to be breeding sites shared by brown frogs and the common toad. DNA barcoding verified Rana dalmatina, R. temporaria and Bufo bufo species, each collected in different location. The results of linear morphometric analyses suggested that relative head length and head width could be good discriminative characteristics for tadpoles of these two Rana species and those of B. bufo. To distinguish between tadpoles of two analyzed brown frog species, relative tail length could be used. For further development of the identification procedures for tadpoles of particular species,
T2  - Applied Ecology and Environmental Research
T1  - Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data
IS  - 2
VL  - 14
DO  - 10.15666/aeer/1402_381397
SP  - 381
EP  - 397
ER  - 

@article{
author = "Ilić, Marija and Stamenković, Gorana and Nikolić, Valentina and Marković, Vanja and Marinković, Nikola and Paunović, Momir and Crnobrnja-Isailović, Jelka",
year = "2016",
abstract = "Many European frogs and toads are relatively secretive species and except during breeding season, adults can rarely be seen during time-restricted fieldwork. In contrast, their tadpoles are easy to record and could be very useful in a brief biodiversity assessment. It is important to perform quick and accurate taxonomic identification of tadpoles, yet genetic methods are costly and cannot be routinely applied. We tested suitability of morphometric analysis for taxonomical distinction among tadpoles of early breeding local anuran species. Tadpole samples were collected simultaneously at three different locations in Republic of Serbia (South-eastern Europe) in habitats known to be breeding sites shared by brown frogs and the common toad. DNA barcoding verified Rana dalmatina, R. temporaria and Bufo bufo species, each collected in different location. The results of linear morphometric analyses suggested that relative head length and head width could be good discriminative characteristics for tadpoles of these two Rana species and those of B. bufo. To distinguish between tadpoles of two analyzed brown frog species, relative tail length could be used. For further development of the identification procedures for tadpoles of particular species,",
journal = "Applied Ecology and Environmental Research",
title = "Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data",
number = "2",
volume = "14",
doi = "10.15666/aeer/1402_381397",
pages = "381-397"
}

Ilić, M., Stamenković, G., Nikolić, V., Marković, V., Marinković, N., Paunović, M.,& Crnobrnja-Isailović, J.. (2016). Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data. in Applied Ecology and Environmental Research, 14(2), 381-397.
https://doi.org/10.15666/aeer/1402_381397

Ilić M, Stamenković G, Nikolić V, Marković V, Marinković N, Paunović M, Crnobrnja-Isailović J. Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data. in Applied Ecology and Environmental Research. 2016;14(2):381-397.
doi:10.15666/aeer/1402_381397 .

Ilić, Marija, Stamenković, Gorana, Nikolić, Valentina, Marković, Vanja, Marinković, Nikola, Paunović, Momir, Crnobrnja-Isailović, Jelka, "Identification of syntopic anuran species in early tadpole stages: correspondence between morphometric and genetic data" in Applied Ecology and Environmental Research, 14, no. 2 (2016):381-397,
https://doi.org/10.15666/aeer/1402_381397 . .

TY  - JOUR
AU  - Tovilović-Kovačević, Gordana
AU  - Ristić, Biljana
AU  - Šiljić, Marina
AU  - Nikolić, Valentina
AU  - Kravić-Stevović, Tamara
AU  - Dulović, Marija
AU  - Milenković, Marina
AU  - Knežević, Aleksandra
AU  - Bošnjak, Mihajlo
AU  - Bumbaširević, Vladimir
AU  - Stanojević, Maja
AU  - Trajković, Vladimir
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6345
AB  - We investigated the role of autophagy, a stress-inducible lysosomal self-digestion of cellular components, in modulation of herpes simplex virus type 1 (HSV-1)-triggered death of U251 human glioma cells. HSV-1 caused apoptotic death in U251 cells, characterized by phosphatidylserine externalization, caspase activation and DNA fragmentation. HSV-1-induced apoptosis was associated with the induction of autophagic response, as confirmed by the conversion of cytosolic LC3-I to autophagosome-associated LC3-II, increase in intracellular acidification, presence of autophagic vesicles, and increase in proteolysis of the selective autophagic target p62. HSV-1-triggered autophagy was not associated with the significant increase in the expression of proautophagic protein beclin-1 or downregulation of the major autophagy suppressor mammalian target of rapamycin (mTOR). Moreover, the phosphorylation of mTOR and its direct substrate p70 S6 kinase was augmented by HSV-1 infection, while the mTOR stimulator Akt and inhibitor AMPK-activated protein kinase (AMPK) were accordingly activated and suppressed, respectively. An shRNA-mediated knockdown of the autophagy-essential LC3b, as well as pharmacological inhibition of autophagy with bafilomycin A1 or 3-methyladenine, markedly accelerated apoptotic changes and ensuing cell death in HSV-1-infected glioma cells. These data indicate that AMPK/Akt/mTOR-independent autophagy could prolong survival of HSV-1-infected U251 glioma cells by counteracting the coinciding apoptotic response.
PB  - Elsevier Masson SAS
T2  - Microbes and Infection
T1  - mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells
IS  - 8-9
VL  - 15
DO  - 10.1016/j.micinf.2013.04.012
SP  - 615
EP  - 624
ER  - 

@article{
author = "Tovilović-Kovačević, Gordana and Ristić, Biljana and Šiljić, Marina and Nikolić, Valentina and Kravić-Stevović, Tamara and Dulović, Marija and Milenković, Marina and Knežević, Aleksandra and Bošnjak, Mihajlo and Bumbaširević, Vladimir and Stanojević, Maja and Trajković, Vladimir",
year = "2013",
abstract = "We investigated the role of autophagy, a stress-inducible lysosomal self-digestion of cellular components, in modulation of herpes simplex virus type 1 (HSV-1)-triggered death of U251 human glioma cells. HSV-1 caused apoptotic death in U251 cells, characterized by phosphatidylserine externalization, caspase activation and DNA fragmentation. HSV-1-induced apoptosis was associated with the induction of autophagic response, as confirmed by the conversion of cytosolic LC3-I to autophagosome-associated LC3-II, increase in intracellular acidification, presence of autophagic vesicles, and increase in proteolysis of the selective autophagic target p62. HSV-1-triggered autophagy was not associated with the significant increase in the expression of proautophagic protein beclin-1 or downregulation of the major autophagy suppressor mammalian target of rapamycin (mTOR). Moreover, the phosphorylation of mTOR and its direct substrate p70 S6 kinase was augmented by HSV-1 infection, while the mTOR stimulator Akt and inhibitor AMPK-activated protein kinase (AMPK) were accordingly activated and suppressed, respectively. An shRNA-mediated knockdown of the autophagy-essential LC3b, as well as pharmacological inhibition of autophagy with bafilomycin A1 or 3-methyladenine, markedly accelerated apoptotic changes and ensuing cell death in HSV-1-infected glioma cells. These data indicate that AMPK/Akt/mTOR-independent autophagy could prolong survival of HSV-1-infected U251 glioma cells by counteracting the coinciding apoptotic response.",
publisher = "Elsevier Masson SAS",
journal = "Microbes and Infection",
title = "mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells",
number = "8-9",
volume = "15",
doi = "10.1016/j.micinf.2013.04.012",
pages = "615-624"
}

Tovilović-Kovačević, G., Ristić, B., Šiljić, M., Nikolić, V., Kravić-Stevović, T., Dulović, M., Milenković, M., Knežević, A., Bošnjak, M., Bumbaširević, V., Stanojević, M.,& Trajković, V.. (2013). mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells. in Microbes and Infection
Elsevier Masson SAS., 15(8-9), 615-624.
https://doi.org/10.1016/j.micinf.2013.04.012

Tovilović-Kovačević G, Ristić B, Šiljić M, Nikolić V, Kravić-Stevović T, Dulović M, Milenković M, Knežević A, Bošnjak M, Bumbaširević V, Stanojević M, Trajković V. mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells. in Microbes and Infection. 2013;15(8-9):615-624.
doi:10.1016/j.micinf.2013.04.012 .

Tovilović-Kovačević, Gordana, Ristić, Biljana, Šiljić, Marina, Nikolić, Valentina, Kravić-Stevović, Tamara, Dulović, Marija, Milenković, Marina, Knežević, Aleksandra, Bošnjak, Mihajlo, Bumbaširević, Vladimir, Stanojević, Maja, Trajković, Vladimir, "mTOR-independent autophagy counteracts apoptosis in herpes simplex virus type 1-infected U251 glioma cells" in Microbes and Infection, 15, no. 8-9 (2013):615-624,
https://doi.org/10.1016/j.micinf.2013.04.012 . .