TY  - JOUR
AU  - Marković, Snežana D.
AU  - Đorđević, Nataša Z.
AU  - Ćurčić, Milena G.
AU  - Štajn, Andraš Š.
AU  - Spasić, Mihajlo
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2151
AB  - The effects of nitroglycerin (glyceryl trinitrate - GTN) are mediated by
   liberated nitric oxide (NO) and formed reactive nitrogen species, which
   induces oxidative stress during biotransformation in red blood cells
   (RBCs). The aim of this study was to evaluate effects of GTN on
   antioxidative defense system (AOS) in rat erythrocytes (without) and
   reticulocytes (with functional mitochondria). Rat erythrocyte and
   reticulocyte-rich RBC suspensions were aerobically incubated (2 h, 37
   degrees C) without (control) or in the presence of different
   concentrations of GTN (0.1-1.5 mM). After incubation, concentrations of
   non-enzymatic components of AOS, activities of antioxidative enzymes and
   oxidative pentose phosphate (OPP) pathway activity were followed in RBC
   suspensions. In rat reticulocytes, GTN decreased the activity of
   mitochondrial MnSOD and increased the activity of CuZnSOD. In rat RBCs,
   GTN induced increase of Vit E concentration (at high doses), but
   decreased glutathione content and activities of all
   glutathione-dependent antioxidative enzymes; the OPP pathway activity
   significantly increased. GTN biotransformation and induction of
   oxidative stress were followed by general disbalance of antioxidative
   capacities in both kinds of RBCs. We suggest that oxidative stress,
   MnSOD inhibition and depletion of glutathione pool in response to GTN
   treatment lead to decreased bioavailability of NO after GTN
   biotransformation in rat reticulocytes.
T2  - General Physiology and Biophysics
T1  - Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes
IS  - 4
VL  - 33
DO  - 10.4149/gpb_2014018
SP  - 393
EP  - 401
ER  - 

@article{
author = "Marković, Snežana D. and Đorđević, Nataša Z. and Ćurčić, Milena G. and Štajn, Andraš Š. and Spasić, Mihajlo",
year = "2014",
abstract = "The effects of nitroglycerin (glyceryl trinitrate - GTN) are mediated by
   liberated nitric oxide (NO) and formed reactive nitrogen species, which
   induces oxidative stress during biotransformation in red blood cells
   (RBCs). The aim of this study was to evaluate effects of GTN on
   antioxidative defense system (AOS) in rat erythrocytes (without) and
   reticulocytes (with functional mitochondria). Rat erythrocyte and
   reticulocyte-rich RBC suspensions were aerobically incubated (2 h, 37
   degrees C) without (control) or in the presence of different
   concentrations of GTN (0.1-1.5 mM). After incubation, concentrations of
   non-enzymatic components of AOS, activities of antioxidative enzymes and
   oxidative pentose phosphate (OPP) pathway activity were followed in RBC
   suspensions. In rat reticulocytes, GTN decreased the activity of
   mitochondrial MnSOD and increased the activity of CuZnSOD. In rat RBCs,
   GTN induced increase of Vit E concentration (at high doses), but
   decreased glutathione content and activities of all
   glutathione-dependent antioxidative enzymes; the OPP pathway activity
   significantly increased. GTN biotransformation and induction of
   oxidative stress were followed by general disbalance of antioxidative
   capacities in both kinds of RBCs. We suggest that oxidative stress,
   MnSOD inhibition and depletion of glutathione pool in response to GTN
   treatment lead to decreased bioavailability of NO after GTN
   biotransformation in rat reticulocytes.",
journal = "General Physiology and Biophysics",
title = "Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes",
number = "4",
volume = "33",
doi = "10.4149/gpb_2014018",
pages = "393-401"
}

Marković, S. D., Đorđević, N. Z., Ćurčić, M. G., Štajn, A. Š.,& Spasić, M.. (2014). Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes. in General Physiology and Biophysics, 33(4), 393-401.
https://doi.org/10.4149/gpb_2014018

Marković SD, Đorđević NZ, Ćurčić MG, Štajn AŠ, Spasić M. Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes. in General Physiology and Biophysics. 2014;33(4):393-401.
doi:10.4149/gpb_2014018 .

Marković, Snežana D., Đorđević, Nataša Z., Ćurčić, Milena G., Štajn, Andraš Š., Spasić, Mihajlo, "Biotransformation and nitroglycerin-induced effects on antioxidative
 defense system in rat erythrocytes and reticulocytes" in General Physiology and Biophysics, 33, no. 4 (2014):393-401,
https://doi.org/10.4149/gpb_2014018 . .