TY  - JOUR
AU  - Miler, Irena
AU  - Rabasović, Mihailo D.
AU  - Aleksić, Marija
AU  - Krmpot, Aleksandar J.
AU  - Kalezić, Anđelika
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jbio.202000362
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4072
AB  - Our previous study on rat skin showed that cumulative oxidative pressure induces profound structural and ultrastructural alterations in both rat skin epidermis and dermis during aging. Here, we aimed to investigate the biophotonic properties of collagen as a main dermal component in the function of chronological aging. We used second harmonic generation (SHG) and two‐photon excited fluorescence (TPEF) on 5 μm thick skin paraffin sections from 15‐day‐, 1‐month‐ and 21‐month‐old rats, respectively, to analyze collagen alterations, in comparison to conventional light and electron microscopy methods. Obtained results show that polarization‐resolved SHG (PSHG) images can detect collagen fiber alterations in line with chronological aging and that this method is consistent with light and electron microscopy. Moreover, the β coefficient calculated from PSHG images points out that delicate alterations lead to a more ordered structure of collagen molecules due to oxidative damage. The results of this study also open the possibility of successfully applying this fast and label‐free method to previously fixed samples.image
PB  - Wiley
T2  - Journal of Biophotonics
T1  - Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy
DO  - 10.1002/jbio.202000362
ER  - 

@article{
author = "Miler, Irena and Rabasović, Mihailo D. and Aleksić, Marija and Krmpot, Aleksandar J. and Kalezić, Anđelika and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2020",
abstract = "Our previous study on rat skin showed that cumulative oxidative pressure induces profound structural and ultrastructural alterations in both rat skin epidermis and dermis during aging. Here, we aimed to investigate the biophotonic properties of collagen as a main dermal component in the function of chronological aging. We used second harmonic generation (SHG) and two‐photon excited fluorescence (TPEF) on 5 μm thick skin paraffin sections from 15‐day‐, 1‐month‐ and 21‐month‐old rats, respectively, to analyze collagen alterations, in comparison to conventional light and electron microscopy methods. Obtained results show that polarization‐resolved SHG (PSHG) images can detect collagen fiber alterations in line with chronological aging and that this method is consistent with light and electron microscopy. Moreover, the β coefficient calculated from PSHG images points out that delicate alterations lead to a more ordered structure of collagen molecules due to oxidative damage. The results of this study also open the possibility of successfully applying this fast and label‐free method to previously fixed samples.image",
publisher = "Wiley",
journal = "Journal of Biophotonics",
title = "Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy",
doi = "10.1002/jbio.202000362"
}

Miler, I., Rabasović, M. D., Aleksić, M., Krmpot, A. J., Kalezić, A., Janković, A., Korać, B.,& Korać, A.. (2020). Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy. in Journal of Biophotonics
Wiley..
https://doi.org/10.1002/jbio.202000362

Miler I, Rabasović MD, Aleksić M, Krmpot AJ, Kalezić A, Janković A, Korać B, Korać A. Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy. in Journal of Biophotonics. 2020;.
doi:10.1002/jbio.202000362 .

Miler, Irena, Rabasović, Mihailo D., Aleksić, Marija, Krmpot, Aleksandar J., Kalezić, Anđelika, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "Polarization‐resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy" in Journal of Biophotonics (2020),
https://doi.org/10.1002/jbio.202000362 . .