TY  - JOUR
AU  - Ristic, Aleksandar J.
AU  - Savić, Danijela
AU  - Sokic, Dragoslav
AU  - Pristov, Jelena Bogdanovic
AU  - Brkljačić, Jelena
AU  - Bascarevic, Vladimir
AU  - Raicevic, Savo
AU  - Savic, Slobodan
AU  - Spasojevic, Ivan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1960
AB  - ObjectiveTo examine antioxidative system in hippocampi of patients with
   mesial temporal lobe epilepsy associated with hippocampal sclerosis
   (mTLE-HS).
   MethodsActivity and levels of antioxidative enzymescatalase (CAT),
   glutathione peroxidase (GPx), glutathione reductase (GR), manganese
   superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase
   (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS
   patients (mean age 37.7{[}standard deviation] 6.6years) who underwent
   amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from
   five neurologically intact controls (mean age 34.49.0years). Subfield
   and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was
   analyzed in detail using immunohistochemical staining.
   ResultsSclerotic hippocampi showed drastically increased activity of
   hydrogen peroxide-removing enzymes, CAT (p<0.001), GPx (p<0.001), and GR
   (p<0.001), and significantly higher protein levels of CAT (p=0.006), GPx
   (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT
   immunofluorescence was located mainly in neurons in both controls and
   HS. Control hippocampi showed GPx staining in blood vessels and CA
   neurons. In HS, GPx-rich loci, representing bundles of astrocytes,
   emerged in different hippocampal regions, whereas the number of
   GPx-positive vessels was drastically decreased. Neurons with abnormal
   morphology and strong MnSOD immunofluorescence were present in all
   neuronal layers in HS. Small autofluorescent deposits, most likely
   lipofuscin, were observed, along with astrogliosis, in CA1 in HS.
   SignificanceAntioxidative system is upregulated in HS. This documents,
   for the first time, that epileptogenic hippocampi are exposed to
   oxidative stress. Our findings provide a basis for understanding the
   potential involvement of redox alterations in the pathology of epilepsy,
   and may open new pharmacologic perspectives for mTLE-HS treatment.
T2  - Epilepsia
T1  - Hippocampal antioxidative system in mesial temporal lobe epilepsy
IS  - 5
VL  - 56
DO  - 10.1111/epi.12981
SP  - 789
EP  - 799
ER  - 

@article{
author = "Ristic, Aleksandar J. and Savić, Danijela and Sokic, Dragoslav and Pristov, Jelena Bogdanovic and Brkljačić, Jelena and Bascarevic, Vladimir and Raicevic, Savo and Savic, Slobodan and Spasojevic, Ivan",
year = "2015",
abstract = "ObjectiveTo examine antioxidative system in hippocampi of patients with
   mesial temporal lobe epilepsy associated with hippocampal sclerosis
   (mTLE-HS).
   MethodsActivity and levels of antioxidative enzymescatalase (CAT),
   glutathione peroxidase (GPx), glutathione reductase (GR), manganese
   superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase
   (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS
   patients (mean age 37.7{[}standard deviation] 6.6years) who underwent
   amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from
   five neurologically intact controls (mean age 34.49.0years). Subfield
   and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was
   analyzed in detail using immunohistochemical staining.
   ResultsSclerotic hippocampi showed drastically increased activity of
   hydrogen peroxide-removing enzymes, CAT (p<0.001), GPx (p<0.001), and GR
   (p<0.001), and significantly higher protein levels of CAT (p=0.006), GPx
   (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT
   immunofluorescence was located mainly in neurons in both controls and
   HS. Control hippocampi showed GPx staining in blood vessels and CA
   neurons. In HS, GPx-rich loci, representing bundles of astrocytes,
   emerged in different hippocampal regions, whereas the number of
   GPx-positive vessels was drastically decreased. Neurons with abnormal
   morphology and strong MnSOD immunofluorescence were present in all
   neuronal layers in HS. Small autofluorescent deposits, most likely
   lipofuscin, were observed, along with astrogliosis, in CA1 in HS.
   SignificanceAntioxidative system is upregulated in HS. This documents,
   for the first time, that epileptogenic hippocampi are exposed to
   oxidative stress. Our findings provide a basis for understanding the
   potential involvement of redox alterations in the pathology of epilepsy,
   and may open new pharmacologic perspectives for mTLE-HS treatment.",
journal = "Epilepsia",
title = "Hippocampal antioxidative system in mesial temporal lobe epilepsy",
number = "5",
volume = "56",
doi = "10.1111/epi.12981",
pages = "789-799"
}

Ristic, A. J., Savić, D., Sokic, D., Pristov, J. B., Brkljačić, J., Bascarevic, V., Raicevic, S., Savic, S.,& Spasojevic, I.. (2015). Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia, 56(5), 789-799.
https://doi.org/10.1111/epi.12981

Ristic AJ, Savić D, Sokic D, Pristov JB, Brkljačić J, Bascarevic V, Raicevic S, Savic S, Spasojevic I. Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia. 2015;56(5):789-799.
doi:10.1111/epi.12981 .

Ristic, Aleksandar J., Savić, Danijela, Sokic, Dragoslav, Pristov, Jelena Bogdanovic, Brkljačić, Jelena, Bascarevic, Vladimir, Raicevic, Savo, Savic, Slobodan, Spasojevic, Ivan, "Hippocampal antioxidative system in mesial temporal lobe epilepsy" in Epilepsia, 56, no. 5 (2015):789-799,
https://doi.org/10.1111/epi.12981 . .

TY  - JOUR
AU  - Milic, Sonja
AU  - Pristov, Jelena Bogdanovic
AU  - Mutavdzic, Dragosav
AU  - Savic, Aleksandar
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1971
AB  - Herein we compared antioxidative activities (AA) of 25 free L-amino
   acids (FAA) against Fenton system-mediated hydroxyl radical (HO center
   dot) production in aqueous solution, and examined the relation between
   AA and a set of physicochemical properties. The tank order according -to
   AA was: Tip > notleucine > Phe, Lett > Ile > His
   >3,4-dihydrOxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydtoxyproline
   > alpha-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp.
   Sulfur-containing FAA generated different secondary reactive products,
   which were discriminated by the means of electron paramagnetic resonance
   spin-trapping spectroscopy. AA showed a general positive correlation
   with hydrophobicity. However, when taken separately, uncharged FAA
   exhibited strong positive correlation of AA with hydrophobicity whereas
   charged FAA showed negative or no significant correlation depending on
   the scale applied. A general strong negative correlation was found
   between AA and polarity. Steric parameters and hydration numbers
   correlated positively with AA of rtoripolar side-chain FAA. In addition)
   a decrease of temperature which promotes hydrophobic hydration resulted
   in increased AA. This implies that HO-provoked oxidation of FAA is
   strongly affected by hydrophobic hydration. Our findings are important
   for the understanding of oxidation processes in natural and waste
   waters.
T2  - Environmental Science & Technology
T1  - The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System
IS  - 7
VL  - 49
DO  - 10.1021/es5053396
SP  - 4245
EP  - 4254
ER  - 

@article{
author = "Milic, Sonja and Pristov, Jelena Bogdanovic and Mutavdzic, Dragosav and Savic, Aleksandar and Spasić, Mihajlo and Spasojevic, Ivan",
year = "2015",
abstract = "Herein we compared antioxidative activities (AA) of 25 free L-amino
   acids (FAA) against Fenton system-mediated hydroxyl radical (HO center
   dot) production in aqueous solution, and examined the relation between
   AA and a set of physicochemical properties. The tank order according -to
   AA was: Tip > notleucine > Phe, Lett > Ile > His
   >3,4-dihydrOxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydtoxyproline
   > alpha-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp.
   Sulfur-containing FAA generated different secondary reactive products,
   which were discriminated by the means of electron paramagnetic resonance
   spin-trapping spectroscopy. AA showed a general positive correlation
   with hydrophobicity. However, when taken separately, uncharged FAA
   exhibited strong positive correlation of AA with hydrophobicity whereas
   charged FAA showed negative or no significant correlation depending on
   the scale applied. A general strong negative correlation was found
   between AA and polarity. Steric parameters and hydration numbers
   correlated positively with AA of rtoripolar side-chain FAA. In addition)
   a decrease of temperature which promotes hydrophobic hydration resulted
   in increased AA. This implies that HO-provoked oxidation of FAA is
   strongly affected by hydrophobic hydration. Our findings are important
   for the understanding of oxidation processes in natural and waste
   waters.",
journal = "Environmental Science & Technology",
title = "The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System",
number = "7",
volume = "49",
doi = "10.1021/es5053396",
pages = "4245-4254"
}

Milic, S., Pristov, J. B., Mutavdzic, D., Savic, A., Spasić, M.,& Spasojevic, I.. (2015). The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System. in Environmental Science & Technology, 49(7), 4245-4254.
https://doi.org/10.1021/es5053396

Milic S, Pristov JB, Mutavdzic D, Savic A, Spasić M, Spasojevic I. The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System. in Environmental Science & Technology. 2015;49(7):4245-4254.
doi:10.1021/es5053396 .

Milic, Sonja, Pristov, Jelena Bogdanovic, Mutavdzic, Dragosav, Savic, Aleksandar, Spasić, Mihajlo, Spasojevic, Ivan, "The Relationship of Physicochemical Properties to the Antioxidative
 Activity of Free Amino Acids in Fenton System" in Environmental Science & Technology, 49, no. 7 (2015):4245-4254,
https://doi.org/10.1021/es5053396 . .

TY  - JOUR
AU  - Mojić, Marija
AU  - Bogdanović Pristov, Jelena
AU  - Maksimović-Ivanić, Danijela
AU  - Jones, David R.
AU  - Stanic, Marina
AU  - Mijatović, Sanja
AU  - Spasojevic, Ivan
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2177
AB  - In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.
T2  - Scientific Reports
T1  - Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study
IS  - 5955
VL  - 4
DO  - 10.1038/srep05955
ER  - 

@article{
author = "Mojić, Marija and Bogdanović Pristov, Jelena and Maksimović-Ivanić, Danijela and Jones, David R. and Stanic, Marina and Mijatović, Sanja and Spasojevic, Ivan",
year = "2014",
abstract = "In vitro studies have shown that hydrogen peroxide (H2O2) produced by
   high-concentration ascorbate and cell culture medium iron efficiently
   kills cancer cells. This provided the rationale for clinical trials of
   high-dose intravenous ascorbate-based treatment for cancer. A drawback
   in all the in vitro studies was their failure to take into account the
   in vivo concentration of iron to supplement cell culture media which are
   characterized by low iron content. Here we showed, using two prostate
   cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the
   anticancer/cytotoxic effects of ascorbate are completely abolished by
   iron at physiological concentrations in cell culture medium and human
   plasma. A detailed examination of mechanisms showed that iron at
   physiological concentrations promotes both production and decomposition
   of H2O2. The latter is mediated by Fenton reaction and prevents H2O2
   accumulation. The hydroxyl radical, which is produced in the Fenton
   reaction, is buffered by extracellular proteins, and could not affect
   intracellular targets like H2O2. These findings show that anticancer
   effects of ascorbate have been significantly overestimated in previous
   in vitro studies, and that common cell culture media might be unsuitable
   for redox research.",
journal = "Scientific Reports",
title = "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study",
number = "5955",
volume = "4",
doi = "10.1038/srep05955"
}

Mojić, M., Bogdanović Pristov, J., Maksimović-Ivanić, D., Jones, D. R., Stanic, M., Mijatović, S.,& Spasojevic, I.. (2014). Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports, 4(5955).
https://doi.org/10.1038/srep05955

Mojić M, Bogdanović Pristov J, Maksimović-Ivanić D, Jones DR, Stanic M, Mijatović S, Spasojevic I. Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study. in Scientific Reports. 2014;4(5955).
doi:10.1038/srep05955 .

Mojić, Marija, Bogdanović Pristov, Jelena, Maksimović-Ivanić, Danijela, Jones, David R., Stanic, Marina, Mijatović, Sanja, Spasojevic, Ivan, "Extracellular iron diminishes anticancer effects of vitamin C: An in
 vitro study" in Scientific Reports, 4, no. 5955 (2014),
https://doi.org/10.1038/srep05955 . .