TY  - JOUR
AU  - Nestorović Živković, Jasmina
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Maksimović, Vuk M
AU  - Grubišić, Dragoljub V.
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1122
AB  - Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential. (C) 2010 Elsevier B.V. All rights reserved.
T2  - Fitoterapia
T1  - Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity
IS  - 6
VL  - 81
SP  - 1
EP  - 626
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1122
ER  - 

@article{
author = "Nestorović Živković, Jasmina and Mišić, Danijela and Šiler, Branislav and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Maksimović, Vuk M and Grubišić, Dragoljub V.",
year = "2010",
abstract = "Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential. (C) 2010 Elsevier B.V. All rights reserved.",
journal = "Fitoterapia",
title = "Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity",
number = "6",
volume = "81",
pages = "1-626",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1122"
}

Nestorović Živković, J., Mišić, D., Šiler, B., Soković, M., Glamočlija, J., Ćirić, A., Maksimović, V. M.,& Grubišić, D. V.. (2010). Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity. in Fitoterapia, 81(6), 1-626.
https://hdl.handle.net/21.15107/rcub_ibiss_1122

Nestorović Živković J, Mišić D, Šiler B, Soković M, Glamočlija J, Ćirić A, Maksimović VM, Grubišić DV. Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity. in Fitoterapia. 2010;81(6):1-626.
https://hdl.handle.net/21.15107/rcub_ibiss_1122 .

Nestorović Živković, Jasmina, Mišić, Danijela, Šiler, Branislav, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Maksimović, Vuk M, Grubišić, Dragoljub V., "Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity" in Fitoterapia, 81, no. 6 (2010):1-626,
https://hdl.handle.net/21.15107/rcub_ibiss_1122 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Maksimović, Vuk M
AU  - Todorović, Slađana
AU  - Grubišić, Dragoljub V.
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1719
AB  - A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.
T2  - Israel Journal of Plant Sciences
T1  - Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro
IS  - 2
VL  - 53
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1719
ER  - 

@article{
author = "Mišić, Danijela and Maksimović, Vuk M and Todorović, Slađana and Grubišić, Dragoljub V. and Konjević, Radomir M",
year = "2005",
abstract = "A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.",
journal = "Israel Journal of Plant Sciences",
title = "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro",
number = "2",
volume = "53",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1719"
}

Mišić, D., Maksimović, V. M., Todorović, S., Grubišić, D. V.,& Konjević, R. M.. (2005). Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences, 53(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1719

Mišić D, Maksimović VM, Todorović S, Grubišić DV, Konjević RM. Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences. 2005;53(2):null-108.
https://hdl.handle.net/21.15107/rcub_ibiss_1719 .

Mišić, Danijela, Maksimović, Vuk M, Todorović, Slađana, Grubišić, Dragoljub V., Konjević, Radomir M, "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro" in Israel Journal of Plant Sciences, 53, no. 2 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1719 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Ghalawenji, Nabil
AU  - Grubišić, Dragoljub V.
AU  - Konjević, Radomir M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1722
AB  - A micropropagation protocol was developed for the conservation of critically endangered Serbian perennial Nepeta rtanjensis (Lamiaceae). Rooted shoots were obtained from one-node stem segments and shoot tips on a half-strength Murashige and Skoog (MS) medium without growth regulators. The best pH of the medium for axillary buds induction and for rooting of shoots was found to be at 7 and/or 7.2 respectively. The addition of cytokinins to the culture medium did not significantly stimulated auxillary bud production as compared to the control. On the contrary, on media supplemented with high cytokinin concentrations, only dwarf shoots with rudimentary roots were obtained. All tested concentrations of 6-benzylaminopyrine (BAP) and kinetine (Kn) in combination with 0.1 mg 1(-1) indole-3-acetic acid (IAA) negatively affected the elongation and rooting of shoots. Plants micropropagated on hormone free medium and rooted in vitro were successfully acclimatized in greenhouse and in open field conditions. The result of successful acclimatization was the production of more than 7000 plantlets with normal sexually reproduction. They flowered, fruited and produced seeds which exhibited 47% germination. The survival rate of plants that were transferred to the open field for the acclimatization and exposed to the winter chill was 99%. The reintroduction of N. rtanjensis occurred in May 2004. One thousand plantlets were planted within the historic range of this plant species. The survival rate was also 99%.
T2  - Phyton-Annales Rei Botanicae
T1  - Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia
IS  - 1
VL  - 45
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1722
ER  - 

@article{
author = "Mišić, Danijela and Ghalawenji, Nabil and Grubišić, Dragoljub V. and Konjević, Radomir M",
year = "2005",
abstract = "A micropropagation protocol was developed for the conservation of critically endangered Serbian perennial Nepeta rtanjensis (Lamiaceae). Rooted shoots were obtained from one-node stem segments and shoot tips on a half-strength Murashige and Skoog (MS) medium without growth regulators. The best pH of the medium for axillary buds induction and for rooting of shoots was found to be at 7 and/or 7.2 respectively. The addition of cytokinins to the culture medium did not significantly stimulated auxillary bud production as compared to the control. On the contrary, on media supplemented with high cytokinin concentrations, only dwarf shoots with rudimentary roots were obtained. All tested concentrations of 6-benzylaminopyrine (BAP) and kinetine (Kn) in combination with 0.1 mg 1(-1) indole-3-acetic acid (IAA) negatively affected the elongation and rooting of shoots. Plants micropropagated on hormone free medium and rooted in vitro were successfully acclimatized in greenhouse and in open field conditions. The result of successful acclimatization was the production of more than 7000 plantlets with normal sexually reproduction. They flowered, fruited and produced seeds which exhibited 47% germination. The survival rate of plants that were transferred to the open field for the acclimatization and exposed to the winter chill was 99%. The reintroduction of N. rtanjensis occurred in May 2004. One thousand plantlets were planted within the historic range of this plant species. The survival rate was also 99%.",
journal = "Phyton-Annales Rei Botanicae",
title = "Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia",
number = "1",
volume = "45",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1722"
}

Mišić, D., Ghalawenji, N., Grubišić, D. V.,& Konjević, R. M.. (2005). Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia. in Phyton-Annales Rei Botanicae, 45(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1722

Mišić D, Ghalawenji N, Grubišić DV, Konjević RM. Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia. in Phyton-Annales Rei Botanicae. 2005;45(1):null-20.
https://hdl.handle.net/21.15107/rcub_ibiss_1722 .

Mišić, Danijela, Ghalawenji, Nabil, Grubišić, Dragoljub V., Konjević, Radomir M, "Micropropagation and reintroduction of Nepeta rtanjensis, an endemic and critically endangered perennial of Serbia" in Phyton-Annales Rei Botanicae, 45, no. 1 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1722 .

TY  - JOUR
AU  - Cvetić, Tijana
AU  - Budimir, Snežana
AU  - Grubišić, Dragoljub V.
PY  - 2004
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/104
T2  - Archives of Biological Sciences
T1  - In vitro flowering of dark-grown Centaurium pulchellum
IS  - 3-4
VL  - 56
SP  - 21
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_104
ER  - 

@article{
author = "Cvetić, Tijana and Budimir, Snežana and Grubišić, Dragoljub V.",
year = "2004, 2004",
journal = "Archives of Biological Sciences",
title = "In vitro flowering of dark-grown Centaurium pulchellum",
number = "3-4",
volume = "56",
pages = "21-22",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_104"
}

Cvetić, T., Budimir, S.,& Grubišić, D. V.. (2004). In vitro flowering of dark-grown Centaurium pulchellum. in Archives of Biological Sciences, 56(3-4), 21-22.
https://hdl.handle.net/21.15107/rcub_ibiss_104

Cvetić T, Budimir S, Grubišić DV. In vitro flowering of dark-grown Centaurium pulchellum. in Archives of Biological Sciences. 2004;56(3-4):21-22.
https://hdl.handle.net/21.15107/rcub_ibiss_104 .

Cvetić, Tijana, Budimir, Snežana, Grubišić, Dragoljub V., "In vitro flowering of dark-grown Centaurium pulchellum" in Archives of Biological Sciences, 56, no. 3-4 (2004):21-22,
https://hdl.handle.net/21.15107/rcub_ibiss_104 .

TY  - JOUR
AU  - Ilić, B.
AU  - Kostić, Elizabeta
AU  - Grubišić, Dragoljub V.
AU  - Ćulafić, Ljubinka
PY  - 2002
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/122
T2  - Arhiv za farmaciju
T1  - Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav
T1  - Multiplication of Silybum marianum (L.) Gaertn. shoots and their flavonoid composition
IS  - 4
VL  - 52
SP  - 806
EP  - 807
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_122
ER  - 

@article{
author = "Ilić, B. and Kostić, Elizabeta and Grubišić, Dragoljub V. and Ćulafić, Ljubinka",
year = "2002, 2002",
journal = "Arhiv za farmaciju",
title = "Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav, Multiplication of Silybum marianum (L.) Gaertn. shoots and their flavonoid composition",
number = "4",
volume = "52",
pages = "806-807",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_122"
}

Ilić, B., Kostić, E., Grubišić, D. V.,& Ćulafić, L.. (2002). Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav. in Arhiv za farmaciju, 52(4), 806-807.
https://hdl.handle.net/21.15107/rcub_ibiss_122

Ilić B, Kostić E, Grubišić DV, Ćulafić L. Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav. in Arhiv za farmaciju. 2002;52(4):806-807.
https://hdl.handle.net/21.15107/rcub_ibiss_122 .

Ilić, B., Kostić, Elizabeta, Grubišić, Dragoljub V., Ćulafić, Ljubinka, "Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav" in Arhiv za farmaciju, 52, no. 4 (2002):806-807,
https://hdl.handle.net/21.15107/rcub_ibiss_122 .