TY  - JOUR
AU  - Banjac, Nevena
AU  - Dmitrović, Slavica
AU  - Đorđević, Mirka
AU  - Zdravković-Korać, Snežana
AU  - Nikolić, Radomirka R
AU  - Raspor, Martin
AU  - Ćosić, Tatjana
AU  - Maksimović, Vuk M
AU  - Živković, Suzana
AU  - Krstić Milošević, Dijana
AU  - Stanišić, Mariana
AU  - Ninković, Slavica
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1150
AB  - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.
T2  - Journal of Plant Physiology
T1  - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
IS  - 12
VL  - 169
DO  - 10.1016/j.jplph.2012.04.009
SP  - 323
EP  - 1211
ER  - 

@article{
author = "Banjac, Nevena and Dmitrović, Slavica and Đorđević, Mirka and Zdravković-Korać, Snežana and Nikolić, Radomirka R and Raspor, Martin and Ćosić, Tatjana and Maksimović, Vuk M and Živković, Suzana and Krstić Milošević, Dijana and Stanišić, Mariana and Ninković, Slavica",
year = "2012",
abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.",
journal = "Journal of Plant Physiology",
title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays",
number = "12",
volume = "169",
doi = "10.1016/j.jplph.2012.04.009",
pages = "323-1211"
}

Banjac, N., Dmitrović, S., Đorđević, M., Zdravković-Korać, S., Nikolić, R. R., Raspor, M., Ćosić, T., Maksimović, V. M., Živković, S., Krstić Milošević, D., Stanišić, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology, 169(12), 323-1211.
https://doi.org/10.1016/j.jplph.2012.04.009

Banjac N, Dmitrović S, Đorđević M, Zdravković-Korać S, Nikolić RR, Raspor M, Ćosić T, Maksimović VM, Živković S, Krstić Milošević D, Stanišić M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):323-1211.
doi:10.1016/j.jplph.2012.04.009 .

Banjac, Nevena, Dmitrović, Slavica, Đorđević, Mirka, Zdravković-Korać, Snežana, Nikolić, Radomirka R, Raspor, Martin, Ćosić, Tatjana, Maksimović, Vuk M, Živković, Suzana, Krstić Milošević, Dijana, Stanišić, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):323-1211,
https://doi.org/10.1016/j.jplph.2012.04.009 . .