TY  - CONF
AU  - Ninković, Slavica
AU  - Banjac, Nevena
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Ćosić, Tatjana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Motyka, Vaclav
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6112
AB  - Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid
PB  - Daejeon: International Association For Plant Biotechnology
C3  - Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
T1  - Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling
SP  - 484
EP  - 484
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6112
ER  - 

@conference{
author = "Ninković, Slavica and Banjac, Nevena and Stanišić, Mariana and Smailagić, Dijana and Ćosić, Tatjana and Živanović, Branka and Dobrev, Petre I. and Motyka, Vaclav",
year = "2023",
abstract = "Root-exuded compounds play a key role in the regulation of plant allelopathic interactions. In contrast to
numerous studies showing that many secondary metabolites have an allelopathic function, evidence of
exudation of phytohormones into the rhizosphere and their involvement in allelopathic interactions is scarce.
We postulated that phytohormones secreted by roots into the environment may act as allelochemicals that
affect the growth performance of neighboring plants. Hairy root culture media of two distinct species, the fruit
tree species Malus domestica cv. Golden delicious[1] and the herbaceous weed species Chenopodium murale
[2,3] were collected and analyzed by ultra-high-performance liquid chromatography coupled with tandem mass
spectrometry (UHPLC-MS/MS). We found that most of plant growth substances exuded by the hairy roots of
C. murale and M. domestica were assorted from fraction A (96.8% and even 98.9%, respectively) including
OxIAA, PAA, SA, BzA, and ABA, with SA and BzA being the most abundant, whereas those from fraction B,
including cytokinins and the ethylene precursor ACC, accounted for only 1% of the regulatory substances
detected in both species. Exogenous application of SA at concentrations released by the hairy roots of the
respective plant species and accumulated in the culture media for four weeks significantly affected the hairy
root growth of the other species and also the shoot and root growth of the Arabidopsis seedlings. The
disruptive effect of SA in the exuded concentration on the membrane potential of Arabidopsis root cells was
determined. The data obtained would be useful for designing further studies aimed at elucidating the
contribution and role of exuded phytohormones to overall allelopathic potential of these two plant species.
OxIAA = 2-oxindole-3-acetic acid, PAA = phenylacetic acid, SA = salicylic acid, BzA = benzoic acid, ABA =
abscisic acid, ACC = 1-aminocyclopropane-1-carboxylic acid",
publisher = "Daejeon: International Association For Plant Biotechnology",
journal = "Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea",
title = "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling",
pages = "484-484",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6112"
}

Ninković, S., Banjac, N., Stanišić, M., Smailagić, D., Ćosić, T., Živanović, B., Dobrev, P. I.,& Motyka, V.. (2023). Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea
Daejeon: International Association For Plant Biotechnology., 484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112

Ninković S, Banjac N, Stanišić M, Smailagić D, Ćosić T, Živanović B, Dobrev PI, Motyka V. Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling. in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea. 2023;:484-484.
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

Ninković, Slavica, Banjac, Nevena, Stanišić, Mariana, Smailagić, Dijana, Ćosić, Tatjana, Živanović, Branka, Dobrev, Petre I., Motyka, Vaclav, "Plant Growth Regulatory Substances Exudation of Hairy Roots of Malus domestica and Chenopodium murale - a Contribution to Their Allelopathic Profiling" in Proceedings Book: The 15th International Association For Plant Biotechnology Congress: Celebrating 50th Anniversary of the Korean Society for Plant Biotechnology; 2023 Aug 6-11; Daejeon, Korea (2023):484-484,
https://hdl.handle.net/21.15107/rcub_ibiss_6112 .

TY  - CONF
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Maksimović, Vuk
AU  - Ninković, Slavica
AU  - Banjac, Nevena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5063
AB  - Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5063
ER  - 

@conference{
author = "Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Maksimović, Vuk and Ninković, Slavica and Banjac, Nevena",
year = "2022",
abstract = "Phloretin, phenolic compound found exclusively in Apple trees, possesses bioherbicidal potential towards other plant species. Our recent studies emphasized phloretin phytotoxic action towards model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh.] as a consequence of disturbed auxin homeostasis in roots and disrupted normal leaf cell function due to chloroplast ultrastructural changes and decreased chlorophyll a and b levels. Given the known phloretin reactivity that results from its flexible dihydrochalcones structure, this study aimed to elucidate phloretin direct effects on the root plasma membrane, as well as phloretin post-treatment metabolism in Arabidopsis plants. Electrophysiological measurements were performed to assess real-time response of root plasma membrane to exogenously applied phloretin. Exposing Arabidopsis roots to a 250 µM phloretin resulted in rapid and significant depolarization of the root membrane potential with amplitude of about 47mV that confirmed high sensitivity of Arabidopsis to phloretin treatment. Reverse-phase liquid chromatography with mass spectrometry (LC-MS) was used to evaluate post-treatment metabolism of phloretin in Arabidopsis seedlings grown on 500 µM phloretin enriched medium for 10 days. Analyses revealed that phloretin underwent intensive glycosylation within plant tissues. Dominant peaks from treated plants showed a palette of phloretin derivatives mainly mono- and di-glycosides. Their quantity was significantly higher than in control plants, suggesting high uptake and metabolic interconversion of phloretin as an attempt of phloretin detoxification and/or preparation for vacuolar sequestration.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings",
pages = "44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5063"
}

Stanišić, M., Smailagić, D., Živanović, B., Maksimović, V., Ninković, S.,& Banjac, N.. (2022). What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063

Stanišić M, Smailagić D, Živanović B, Maksimović V, Ninković S, Banjac N. What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:44.
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Maksimović, Vuk, Ninković, Slavica, Banjac, Nevena, "What happens with phloretin in plants? – Phloretin real-time effects and post-treatment metabolism in treated Arabidopsis seedlings" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):44,
https://hdl.handle.net/21.15107/rcub_ibiss_5063 .

TY  - JOUR
AU  - Bavrina, TV
AU  - Lozhnikova, VN
AU  - Ćulafić, Ljubinka
AU  - Živanović, Branka D
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1793
AB  - Green plants and plants devoid of photosynthetic pigments were compared with regard to their ability to flower under various growth conditions. Green plants of Chenopodium rubrum L. and plants treated with norflurazon SANDOZ-9789 (SAN) were grown on sucrose-containing media with or without hormones (GA(3), BA, IAA, ABA) under short-day photoperiodic or continuous illumination with white, blue, or red light. Green and SAN-treated albino plants produced flowers only under short-day conditions. The flowering of green plants was independent of the presence of sucrose and hormones in the medium as well as of the light quality. The albino plants produced flowers under white and blue light but did not flower in red light. The addition of GA(3) or BA to the medium induced flowering of albino plants exposed to red light. The functional interaction of photoreceptors in the flowering control is discussed.
T2  - Russian Journal of Plant Physiology
T1  - Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light
IS  - 4
VL  - 49
EP  - 464
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1793
ER  - 

@article{
author = "Bavrina, TV and Lozhnikova, VN and Ćulafić, Ljubinka and Živanović, Branka D",
year = "2002",
abstract = "Green plants and plants devoid of photosynthetic pigments were compared with regard to their ability to flower under various growth conditions. Green plants of Chenopodium rubrum L. and plants treated with norflurazon SANDOZ-9789 (SAN) were grown on sucrose-containing media with or without hormones (GA(3), BA, IAA, ABA) under short-day photoperiodic or continuous illumination with white, blue, or red light. Green and SAN-treated albino plants produced flowers only under short-day conditions. The flowering of green plants was independent of the presence of sucrose and hormones in the medium as well as of the light quality. The albino plants produced flowers under white and blue light but did not flower in red light. The addition of GA(3) or BA to the medium induced flowering of albino plants exposed to red light. The functional interaction of photoreceptors in the flowering control is discussed.",
journal = "Russian Journal of Plant Physiology",
title = "Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light",
number = "4",
volume = "49",
pages = "464",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1793"
}

Bavrina, T., Lozhnikova, V., Ćulafić, L.,& Živanović, B. D.. (2002). Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light. in Russian Journal of Plant Physiology, 49(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1793

Bavrina T, Lozhnikova V, Ćulafić L, Živanović BD. Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light. in Russian Journal of Plant Physiology. 2002;49(4):null-464.
https://hdl.handle.net/21.15107/rcub_ibiss_1793 .

Bavrina, TV, Lozhnikova, VN, Ćulafić, Ljubinka, Živanović, Branka D, "Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light" in Russian Journal of Plant Physiology, 49, no. 4 (2002),
https://hdl.handle.net/21.15107/rcub_ibiss_1793 .