TY  - JOUR
AU  - Vidović, Nevena
AU  - Ranković, Slavica
AU  - Oreščanin Dušić, Zorana
AU  - Debeljak-Martačić, Jasmina
AU  - Popović, Tamara
AU  - Tomić, Mirko
AU  - Glibetić, Marija
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0042-84501800122V
UR  - https://radar.ibiss.bg.ac.rs/123456789/3878
AB  - Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ± 0.73 U/mg) and CAT (147.25 ± 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ± 0.11 U/mg; CAT: 99.37 ± 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ± 0.52 U/mg; CAT: 104.18 ± 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ± 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ± 0.08 nmoL/mg; the fish-based diet group: 1.13 ± 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one’s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants
PB  - National Library of Serbia
T2  - Vojnosanitetski pregled
T1  - The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study
IS  - 6
VL  - 77
DO  - 10.2298/VSP180326122V
SP  - 641
EP  - 646
ER  - 

@article{
author = "Vidović, Nevena and Ranković, Slavica and Oreščanin Dušić, Zorana and Debeljak-Martačić, Jasmina and Popović, Tamara and Tomić, Mirko and Glibetić, Marija",
year = "2020",
abstract = "Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ± 0.73 U/mg) and CAT (147.25 ± 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ± 0.11 U/mg; CAT: 99.37 ± 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ± 0.52 U/mg; CAT: 104.18 ± 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ± 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ± 0.08 nmoL/mg; the fish-based diet group: 1.13 ± 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one’s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants",
publisher = "National Library of Serbia",
journal = "Vojnosanitetski pregled",
title = "The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study",
number = "6",
volume = "77",
doi = "10.2298/VSP180326122V",
pages = "641-646"
}

Vidović, N., Ranković, S., Oreščanin Dušić, Z., Debeljak-Martačić, J., Popović, T., Tomić, M.,& Glibetić, M.. (2020). The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study. in Vojnosanitetski pregled
National Library of Serbia., 77(6), 641-646.
https://doi.org/10.2298/VSP180326122V

Vidović N, Ranković S, Oreščanin Dušić Z, Debeljak-Martačić J, Popović T, Tomić M, Glibetić M. The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study. in Vojnosanitetski pregled. 2020;77(6):641-646.
doi:10.2298/VSP180326122V .

Vidović, Nevena, Ranković, Slavica, Oreščanin Dušić, Zorana, Debeljak-Martačić, Jasmina, Popović, Tamara, Tomić, Mirko, Glibetić, Marija, "The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study" in Vojnosanitetski pregled, 77, no. 6 (2020):641-646,
https://doi.org/10.2298/VSP180326122V . .

TY  - JOUR
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Petrović, Snježana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Maria
PY  - 2017
UR  - http://lipidworld.biomedcentral.com/articles/10.1186/s12944-017-0483-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2768
AB  - Background: Dietary intake influence changes in fatty acids (FA) profiles in liver which plays a central role in fatty acid metabolism, triacylglycerol synthesis and energy homeostasis. We investigated the effects of 4-weeks treatment with milk- and fish-based diet, on plasma biochemical parameters and FA composition of liver phospholipids (PL) in rats of both sexes. Methods: Adult, 4 months old, Wistar rats of both sexes, were fed with different types of diets: standard, milk-based and fish-based, during 4 weeks. Analytical characterization of different foods was done. Biochemical parameters in plasma were determined. Fatty acid composition was analyzed by gas-chromatography. Statistical significance of FA levels was tested with two-way analysis of variance (ANOVA) using the sex of animals and treatment (type of diet) as factors on logarithmic or trigonometric transformed data. Results: Our results showed that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Initially present sex differences appear to be dietary modulated. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), and effects were gender specific. Milk-based diet lowered SFA and elevated MUFA in males and increased PUFA in females vs. standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males. Fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio vs. standard and milk-based diet in females. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males. Conclusion: Gender related variations in FA composition of rat liver PL were observed, and results have shown that those initial differences could be significantly modulated by the type of diet. Furthermore, the modulatory effects of milk- and fish-based diets on liver phospholipids FA profiles appeared to be sex-specific.
T2  - Lipids in Health and Disease
T1  - Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes
IS  - 1
VL  - 16
DO  - 10.1186/s12944-017-0483-9
SP  - 94
EP  - 94
ER  - 

@article{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Petrović, Snježana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Maria",
year = "2017",
abstract = "Background: Dietary intake influence changes in fatty acids (FA) profiles in liver which plays a central role in fatty acid metabolism, triacylglycerol synthesis and energy homeostasis. We investigated the effects of 4-weeks treatment with milk- and fish-based diet, on plasma biochemical parameters and FA composition of liver phospholipids (PL) in rats of both sexes. Methods: Adult, 4 months old, Wistar rats of both sexes, were fed with different types of diets: standard, milk-based and fish-based, during 4 weeks. Analytical characterization of different foods was done. Biochemical parameters in plasma were determined. Fatty acid composition was analyzed by gas-chromatography. Statistical significance of FA levels was tested with two-way analysis of variance (ANOVA) using the sex of animals and treatment (type of diet) as factors on logarithmic or trigonometric transformed data. Results: Our results showed that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Initially present sex differences appear to be dietary modulated. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), and effects were gender specific. Milk-based diet lowered SFA and elevated MUFA in males and increased PUFA in females vs. standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males. Fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio vs. standard and milk-based diet in females. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males. Conclusion: Gender related variations in FA composition of rat liver PL were observed, and results have shown that those initial differences could be significantly modulated by the type of diet. Furthermore, the modulatory effects of milk- and fish-based diets on liver phospholipids FA profiles appeared to be sex-specific.",
journal = "Lipids in Health and Disease",
title = "Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes",
number = "1",
volume = "16",
doi = "10.1186/s12944-017-0483-9",
pages = "94-94"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Petrović, S., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2017). Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. in Lipids in Health and Disease, 16(1), 94-94.
https://doi.org/10.1186/s12944-017-0483-9

Ranković S, Popović T, Debeljak Martačić J, Petrović S, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. in Lipids in Health and Disease. 2017;16(1):94-94.
doi:10.1186/s12944-017-0483-9 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Petrović, Snježana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Maria, "Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes" in Lipids in Health and Disease, 16, no. 1 (2017):94-94,
https://doi.org/10.1186/s12944-017-0483-9 . .

TY  - JOUR
AU  - Zec, Manja
AU  - Debeljak Martačić, Jasmina
AU  - Ranković, Slavica
AU  - Pokimica, Biljana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Glibetić, Maria
AU  - Popović, Tamara
PY  - 2017
UR  - http://www.degruyter.com/view/j/acve.2017.67.issue-1/acve-2017-0010/acve-2017-0010.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2731
AB  - A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (1/4-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ significantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of beneficial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, significant increase (P < 0.001) in cis-vaccenic acid (VA, 18:1n-7) in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These findings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.
T2  - Acta Veterinaria
T1  - Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats
IS  - 1
VL  - 67
DO  - 10.1515/acve-2017-0010
SP  - 107
EP  - 120
ER  - 

@article{
author = "Zec, Manja and Debeljak Martačić, Jasmina and Ranković, Slavica and Pokimica, Biljana and Tomić, Mirko and Ignjatović, Đurđica and Glibetić, Maria and Popović, Tamara",
year = "2017",
abstract = "A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (1/4-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ significantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of beneficial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, significant increase (P < 0.001) in cis-vaccenic acid (VA, 18:1n-7) in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These findings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.",
journal = "Acta Veterinaria",
title = "Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats",
number = "1",
volume = "67",
doi = "10.1515/acve-2017-0010",
pages = "107-120"
}

Zec, M., Debeljak Martačić, J., Ranković, S., Pokimica, B., Tomić, M., Ignjatović, Đ., Glibetić, M.,& Popović, T.. (2017). Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats. in Acta Veterinaria, 67(1), 107-120.
https://doi.org/10.1515/acve-2017-0010

Zec M, Debeljak Martačić J, Ranković S, Pokimica B, Tomić M, Ignjatović Đ, Glibetić M, Popović T. Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats. in Acta Veterinaria. 2017;67(1):107-120.
doi:10.1515/acve-2017-0010 .

Zec, Manja, Debeljak Martačić, Jasmina, Ranković, Slavica, Pokimica, Biljana, Tomić, Mirko, Ignjatović, Đurđica, Glibetić, Maria, Popović, Tamara, "Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats" in Acta Veterinaria, 67, no. 1 (2017):107-120,
https://doi.org/10.1515/acve-2017-0010 . .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Pokimica, Biljana
AU  - Petrović-Oggiano, Gordana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Marija
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6770
AB  - Gender related differences in fatty acid composition of different lipid classes are well established. Changes in the proportions of specific long-chain PUFA in membrane phospholipids can alter cell and tissue function.
	The aim of our study was to examine the effects of different diets on phospholipids fatty acids composition in rat's liver of both sexes and to compare them.
	Adult Wistar rats of both sexes (n=8, 300g+5) were used in this study. Animals were fed with different type of diets (standard, fish-based and milk- based). The phospholipids fatty acid composition of liver was determined by Gas Chromatography using Shimadzu GC.
	Diet changes the ratio of SFA, MUFA and PUFA but the effects were sex specific. Milk based diet lowered the amount of SFA (p<0.05) and elevated the ratio of MUFA (ANOVA diet effect, p<0.001) in males (ANOVA sex effect p<0.001, and interaction S x D, p<0.05), and PUFA, in females (ANOVA sex effect p<0.05, and diet effect p<0.05) comparing to standard diet. Milk based diet decreased n-3 (p<0.001), and increased n-6 (p<0.001) and n-6/n-3 (p<0.001) ratio in males comparing to the other type of diets. On the other hand, in females, fish based diet increased n-3 (p<0.001), decreased n-6 (p<0.001) and n-6/n-3 ratio (p<0.001) comparing to standard and milk based diets (ANOVA diet effect, p< 0.001, S x D effect, p<0.001, Tukey's post hoc).
	Our results showed that each type of diet changes the composition of lipids and their ratio in the liver of rats. Fish based diet improving EPA and DHA fatty acid profiles and overall PUFA and has beneficial health effect.
PB  - Belgrade: Serbian Nutrition Society
C3  - Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia
T1  - Effects of different types of diet on liver fatty acids profiles in Wistar rats
SP  - 277
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Pokimica, Biljana and Petrović-Oggiano, Gordana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Marija",
year = "2016",
abstract = "Gender related differences in fatty acid composition of different lipid classes are well established. Changes in the proportions of specific long-chain PUFA in membrane phospholipids can alter cell and tissue function.
	The aim of our study was to examine the effects of different diets on phospholipids fatty acids composition in rat's liver of both sexes and to compare them.
	Adult Wistar rats of both sexes (n=8, 300g+5) were used in this study. Animals were fed with different type of diets (standard, fish-based and milk- based). The phospholipids fatty acid composition of liver was determined by Gas Chromatography using Shimadzu GC.
	Diet changes the ratio of SFA, MUFA and PUFA but the effects were sex specific. Milk based diet lowered the amount of SFA (p<0.05) and elevated the ratio of MUFA (ANOVA diet effect, p<0.001) in males (ANOVA sex effect p<0.001, and interaction S x D, p<0.05), and PUFA, in females (ANOVA sex effect p<0.05, and diet effect p<0.05) comparing to standard diet. Milk based diet decreased n-3 (p<0.001), and increased n-6 (p<0.001) and n-6/n-3 (p<0.001) ratio in males comparing to the other type of diets. On the other hand, in females, fish based diet increased n-3 (p<0.001), decreased n-6 (p<0.001) and n-6/n-3 ratio (p<0.001) comparing to standard and milk based diets (ANOVA diet effect, p< 0.001, S x D effect, p<0.001, Tukey's post hoc).
	Our results showed that each type of diet changes the composition of lipids and their ratio in the liver of rats. Fish based diet improving EPA and DHA fatty acid profiles and overall PUFA and has beneficial health effect.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia",
title = "Effects of different types of diet on liver fatty acids profiles in Wistar rats",
pages = "277"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Pokimica, B., Petrović-Oggiano, G., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2016). Effects of different types of diet on liver fatty acids profiles in Wistar rats. in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia
Belgrade: Serbian Nutrition Society., 277.

Ranković S, Popović T, Debeljak Martačić J, Pokimica B, Petrović-Oggiano G, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Effects of different types of diet on liver fatty acids profiles in Wistar rats. in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia. 2016;:277..

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Pokimica, Biljana, Petrović-Oggiano, Gordana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Marija, "Effects of different types of diet on liver fatty acids profiles in Wistar rats" in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia (2016):277.

TY  - CONF
AU  - Popović, Tamara
AU  - Ranković, Slavica
AU  - Debeljak Martačić, Jasmina
AU  - Pokimica, Biljana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6769
AB  - Aronia melanocarpa (AM) is a medicinal plant commonly named black chokeberry and investigated for wide-array of health-promoting characteristics. The beneficial properties of AM on human health are attributed to high content of non-nutritional phytochemicals i.e. polyphenols. The AMJ corresponded to commercial aronia juice product, available on the Serbian market. A commercial product used has been registered as dietary supplement by Serbian Ministry of Health, under the trade name "Aronia Anti-Oxi" (515-04-1339/2011-04).
The animals were devided in 2 groups of 8 animals each, to receive ad libitum either full polyphenol aronia dose (AMJ), and polyphenol- lacking placebo beverage (PLB).
The animals were kept in groups of 4 per cage, under controlled conditions (room temperature 23-25°C, 12 h light-dark cycle, food and water ad libitum). All animal procedures were in compliance with Directive 2010/63/EU on the protection of animals used for experimental and other scientific purposes, and approved by the Ethical Committee for the Use of Laboratory Animals of the Institute for Biological Research "Siniša Stanković".
The extraction and preparation of FA esters and GC analyses were performed (Folch 1957). Methyl-ester derivatives formed from isolated liver phospholipids fraction were separated by GC (Shimadzu Co, Tokyo, Japan)
Our results showed decreasing in stearic acid (p<0.01), increasing in vaccenic acid (p<0.001) as well as MUFA(p<0.05). Dihomo-gama- linolenic (n-6) acid was increased (p<0.05) while arachidonic acid (n-6) was decreased (p<0.05). n-6/n-3 ratio was decreased but not significantly. Liver enzyme activities were significantly different for elongase (p = 0.009) and D5D enzyme (p = 0.015), due to the decreasing effect of aronia juice consumption in comparison with control.
Stronger effect of aronia juice in liver, as a primary site of FA metabolism and through the decreasing in arachidonic acid, as well as decline in FA metabolic enzymes indicates favorable lipid-metabolism related properties of AMJ consumption.
PB  - Belgrade: Serbian Nutrition Society
C3  - Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia
T1  - Effect of commercially available polyphenol-rich Aronia juice on liver fatty acids phospholipids in Wistar rats
SP  - 275
EP  - 276
ER  - 

@conference{
author = "Popović, Tamara and Ranković, Slavica and Debeljak Martačić, Jasmina and Pokimica, Biljana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana",
year = "2016",
abstract = "Aronia melanocarpa (AM) is a medicinal plant commonly named black chokeberry and investigated for wide-array of health-promoting characteristics. The beneficial properties of AM on human health are attributed to high content of non-nutritional phytochemicals i.e. polyphenols. The AMJ corresponded to commercial aronia juice product, available on the Serbian market. A commercial product used has been registered as dietary supplement by Serbian Ministry of Health, under the trade name "Aronia Anti-Oxi" (515-04-1339/2011-04).
The animals were devided in 2 groups of 8 animals each, to receive ad libitum either full polyphenol aronia dose (AMJ), and polyphenol- lacking placebo beverage (PLB).
The animals were kept in groups of 4 per cage, under controlled conditions (room temperature 23-25°C, 12 h light-dark cycle, food and water ad libitum). All animal procedures were in compliance with Directive 2010/63/EU on the protection of animals used for experimental and other scientific purposes, and approved by the Ethical Committee for the Use of Laboratory Animals of the Institute for Biological Research "Siniša Stanković".
The extraction and preparation of FA esters and GC analyses were performed (Folch 1957). Methyl-ester derivatives formed from isolated liver phospholipids fraction were separated by GC (Shimadzu Co, Tokyo, Japan)
Our results showed decreasing in stearic acid (p<0.01), increasing in vaccenic acid (p<0.001) as well as MUFA(p<0.05). Dihomo-gama- linolenic (n-6) acid was increased (p<0.05) while arachidonic acid (n-6) was decreased (p<0.05). n-6/n-3 ratio was decreased but not significantly. Liver enzyme activities were significantly different for elongase (p = 0.009) and D5D enzyme (p = 0.015), due to the decreasing effect of aronia juice consumption in comparison with control.
Stronger effect of aronia juice in liver, as a primary site of FA metabolism and through the decreasing in arachidonic acid, as well as decline in FA metabolic enzymes indicates favorable lipid-metabolism related properties of AMJ consumption.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia",
title = "Effect of commercially available polyphenol-rich Aronia juice on liver fatty acids phospholipids in Wistar rats",
pages = "275-276"
}

Popović, T., Ranković, S., Debeljak Martačić, J., Pokimica, B., Tomić, M., Ignjatović, Đ.,& Tovilović-Kovačević, G.. (2016). Effect of commercially available polyphenol-rich Aronia juice on liver fatty acids phospholipids in Wistar rats. in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia
Belgrade: Serbian Nutrition Society., 275-276.

Popović T, Ranković S, Debeljak Martačić J, Pokimica B, Tomić M, Ignjatović Đ, Tovilović-Kovačević G. Effect of commercially available polyphenol-rich Aronia juice on liver fatty acids phospholipids in Wistar rats. in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia. 2016;:275-276..

Popović, Tamara, Ranković, Slavica, Debeljak Martačić, Jasmina, Pokimica, Biljana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, "Effect of commercially available polyphenol-rich Aronia juice on liver fatty acids phospholipids in Wistar rats" in Book of Abstracts: 13th Congress of Nutrition Food and Nutrition: A Roadmap to Better Health; 2016 Oct 26-28; Belgrade, Serbia (2016):275-276.

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Petrović Oggiano, Gordana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Miler, Marko
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6347
AB  - Cardiovascular diseases (CVD) are still leading cause of death worldwide. It has been shown that folic acid deficiency can modify polyunsaturated fatty acid (PUFA) metabolism and thus could contribute to the development of (CVD). The contents of (n-6) and (n-3) long-chain PUFA in cell membranes, are important determinants of the biophysical properties of those membranes. The long-chain n-3 PUFAs have been shown to have beneficial effects in delaying the development of various diseases. The aim of our study was to examine the effects of food enriched with fish flour and/or food enriched with fish flour + folic acid on fatty acid composition of liver lipids in 4 weeks treatment. Fe- male Wistar rats were randomly assigned into 3 experimental groups (n=10, 280g±5). Control group received standard food (Veterinarski zavod, Subotica). Both treated groups received a food enriched with fish flour, while in second treated group folic acid was added. After sacrificing, part of the liver was frozen at -80 °C. The phospholipids fatty acid composition of liver was determined by GC using Shimadzu GC. Our results showed that food enriched with fish flour significantly increased: eicosatrienoic (20:3), eicosapentaenoic acid (20:5), docosapentaenoic acid (22:5), docosahexaenoic acid (22:6), n-3 and decreased arachidonic acid, n-6 and n-6/n-3 ratio of liver phospholipids concentrations vs. control. Food enriched with folic acid increased (20:3), (20:5), (22:5), (22:6), PUFA, n-3 and decreased MUFA and palmitic acid (16:0) and n-6/n-3 ratio vs. control. Both type of food significantly decreased concentration of and linoleic (18:2) and vascenic acid (18:1, n-7).
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia
T1  - Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats
SP  - 139
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6347
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Petrović Oggiano, Gordana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Miler, Marko and Glibetić, Marija",
year = "2015",
abstract = "Cardiovascular diseases (CVD) are still leading cause of death worldwide. It has been shown that folic acid deficiency can modify polyunsaturated fatty acid (PUFA) metabolism and thus could contribute to the development of (CVD). The contents of (n-6) and (n-3) long-chain PUFA in cell membranes, are important determinants of the biophysical properties of those membranes. The long-chain n-3 PUFAs have been shown to have beneficial effects in delaying the development of various diseases. The aim of our study was to examine the effects of food enriched with fish flour and/or food enriched with fish flour + folic acid on fatty acid composition of liver lipids in 4 weeks treatment. Fe- male Wistar rats were randomly assigned into 3 experimental groups (n=10, 280g±5). Control group received standard food (Veterinarski zavod, Subotica). Both treated groups received a food enriched with fish flour, while in second treated group folic acid was added. After sacrificing, part of the liver was frozen at -80 °C. The phospholipids fatty acid composition of liver was determined by GC using Shimadzu GC. Our results showed that food enriched with fish flour significantly increased: eicosatrienoic (20:3), eicosapentaenoic acid (20:5), docosapentaenoic acid (22:5), docosahexaenoic acid (22:6), n-3 and decreased arachidonic acid, n-6 and n-6/n-3 ratio of liver phospholipids concentrations vs. control. Food enriched with folic acid increased (20:3), (20:5), (22:5), (22:6), PUFA, n-3 and decreased MUFA and palmitic acid (16:0) and n-6/n-3 ratio vs. control. Both type of food significantly decreased concentration of and linoleic (18:2) and vascenic acid (18:1, n-7).",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia",
title = "Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats",
pages = "139-139",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6347"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Petrović Oggiano, G., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G., Miler, M.,& Glibetić, M.. (2015). Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats. in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia
Belgrade: Serbian Physiology Society., 139-139.
https://hdl.handle.net/21.15107/rcub_ibiss_6347

Ranković S, Popović T, Debeljak Martačić J, Petrović Oggiano G, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Miler M, Glibetić M. Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats. in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia. 2015;:139-139.
https://hdl.handle.net/21.15107/rcub_ibiss_6347 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Petrović Oggiano, Gordana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Miler, Marko, Glibetić, Marija, "Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats" in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia (2015):139-139,
https://hdl.handle.net/21.15107/rcub_ibiss_6347 .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6348
AB  - Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.
PB  - Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology
C3  - Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia
T1  - Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6348
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Marija",
year = "2015",
abstract = "Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.",
publisher = "Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology",
journal = "Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia",
title = "Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6348"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2015). Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats. in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia
Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology., 85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6348

Ranković S, Popović T, Debeljak Martačić J, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats. in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia. 2015;:85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6348 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Marija, "Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats" in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia (2015):85-85,
https://hdl.handle.net/21.15107/rcub_ibiss_6348 .