@conference{
author = "Vuković, Sandra and Milinčić, Danijel and Mačukanović-Jocić, Marina and Kilibarda, Sofija and Jarić, Snežana and Kostić, Aleksandar",
year = "2021",
abstract = "Corydalis ochroleuca Koch. (Papaveraceae) called white corydalis is herbaceous
perennial plant, native to rocky woodland areas of south-eastern Europe. It is recognized by narrow,
tubular, fragrant white-yellow flowers. Currently, use of this plant is mainly associated with decorative
purposes, while there is lack of data for other possibilities of its application.
In this research, rhizome and vegetative shoot of C. ochroleuca collected from canyon of the Derventa
river (National park Tara) located in western Serbia, were used to determine antioxidant activity by
ABTS∙+, DPPH∙, in vitro phosphomolybdenum total antioxidant (TAC), cupric reducing antioxidant
capacity (CUPRAC) and ferric reducing power (FRP) assays. For this purpose, samples were washed,
powdered and extracted with 80% methanol by solvent extraction (SE) and ultrasound-assisted extraction
(UAE).
The aim of this study was to evaluate antioxidant activity of C. ochroleuca extracts and to determine
their correlation with previously spectrophotometrically determined phenolic classes (total phenolics
(TPC), total flavonoids (TFC), total hydroxycinnamic acid derivatives (HCAs)).
The results of antioxidat assays show that the rhizome extract obtained by UAE show the highest
antioxidant activity by ABTS∙+ (58.66% ± 0.72) and CUPRAC (67.30 ± 0.73 mg/g AAE). In the case of
DPPH∙, TAC and FRP assays, extracts of vegetative shoot obtained by both extraction techniques (SE
and UAE) show higher antioxidant activity then rhizome extracts.
Correlation analysis confirmed a positive correlation between TFC and antioxidant tests - DPPH∙ (r=
0.96; p˂ 0.1) and TAC (r= 0.92; p˂ 0.1), and between HCAs and DPPH∙ (r= 0.91; p˂ 0.1), respectively.
DPPH∙ is a known lipophilic and stable free radical, so this result indicates that the extract of the tested
plant was rich in lipophilic flavonoids and chlorogenic acid derivatives that are able to donate H+ ions
and neutralize the DPPH radical.
Conclusion. Also, there was good correlation between DPPH∙ and TAC (r= 0.99; p˂ 0.1). It is assumed
that the total flavonoids, which show the same activity according to these assays, contributed to this
correlation.
In general, flavonoids apparently contribute to high antioxidant activity of C. ochroleuca which indicate
a complex flavonoid profile. Further research should focus on detailed phenolic characterization and
analysis with their in vivo and in vitro properties.",
publisher = "Kharkiv, Ukraine: National University of Pharmacy, Pharmaceutical Chemistry Department",
journal = "International scientific-practical symposium "Century of success and quality", dedicated to the 100th anniversary of Pharmaceutical Chemistry Department of National University of Pharmacy; 2021 Oct 18; Kharkiv, Ukraine",
title = "Correlation between antioxidant activity and phenolics of Corydalis ochroleuca extracts obtained by different extraction techniques",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4907"
}
