TY  - CONF
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Kulaš, Jelena
AU  - Soković Bajić, Svetlana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6113
AB  - Crevna mikrobiota ima važnu ulogu u održavanju homeostaze koju ostvaruje preko interakcija sa imunskim
sistemom i gastrointestinalnim traktom. Toksične supstance ostvaruju direktan štetni efekat na sastav
i funkcionalnost crevne mikrobiote. Kadmijum (Cd) je metal sa izraženom toksičnošću, koji se povezuje
sa brojnim oboljenjima. U našoj studiji je predložen mehanizam zaštite koji bi se ostvario posredstvom
bakterijskih egzopolisaharida (EPS) izolovanih iz soja Lactiplantibacillus plantarum BGAN8, a bazira se
na visokom afinitetu adsorpcije kadmijumovih jona. Ciljevi rada su bili utvrđivanje efekata produženog
delovanja kadmijuma na sastav crevne mikrobiote Dark agouti (DA) pacova, kao i izučavanje efekta
EPS-AN8 na ublažavanje takvih štetnih efekata.
Pacovi su kroz vodu unosili nižu (5 ppm) i višu (50 ppm) dozu kadmijuma, dok je EPS-AN8 (100 μg/ml) dat
kroz hranu za životinje. Totalna DNK je izolovana iz lumena duodenuma i 16S rDNK amplikon je sekvenciran
strategijom uparenih krajeva na Illumina NovaSeq platformi. Dodatno, praćene su promene u
inflamaciji i oksidativnom stresu homogenata duodenuma. Kadmijum je favorizovao rast oportunističkih
patogenih bakterija poput pripadnika rodova Blautia, Prevotella, Alloprevotela, Bacteroides, dok je
relativna zastupljenost laktobacila bila značajno smanjena. Obe doze kadmijuma su stimulisale produkciju
proinflamatornih citokina (IL-1β, TNF-α i IFN-γ) i povisile nivo oksidativnog stresa (MDA, CAT i
GST). Oralni unos EPS-AN8 je značajno ublažio sve navedene promene. Oralna izloženost kadmijumu
uzrokuje nedvosmislene promene u sastavu mikrobiote, koje mogu biti praćene i pojačanim nivoima
inflamacije i oksidativnog stresa. Nasuprot tome, istovremeni unos EPS-AN8 redukuje pojavu takvih
promena.
AB  - The gut microbiota has important role in maintaining homeostasis via its effect on the immune system
and healthy gastrointestinal tract. Environmental toxicants directly influenced the changes in gut microbiota
composition. Cadmium (Cd) is a hazard, world widely distributed metal. We have proposed
the putative mechanism of protection against Cd harmful effect in the organism by using exopolysaccharides
derived from Lactiplantibacillus plantarum BGAN8 (EPS-AN8) which has great ability to sequester Cd ions.
Aims of our study were to examine Cd related changes in the gut microbiota of Dark agouti (DA) rats after
prolonged oral exposure (30 days) to this metal and to determine impact of EPS-AN8 in preventing
those changes.
Rats were given via water lower (5 ppm) and higher (50 ppm) Cd’s dose. In paralel, EPS-AN8 was administrated
through food (100 μg/ml). Total DNA from duodenum was isolated and PCR amplicon for 16SrRNA was
sequenced on Illumina NovaSeq paired end platform. Moreover, we have followed changes in
oxidative stress and inflammation in duodenum. Cd favored growth of opportunistic pathogen bacteria
genera (Blautia, Prevotella, Alloprevotela, Bacteroides) while the relative abundance of lactobacilli was
decreased. The both doses of Cd increased production of inflammatory cytokines (IL-1β, TNF-α, and
IFN-γ). Also, parameters of oxidative stress were significantly upregulated (MDA, CAT, and GST).
All the mentioned changes were alleviated by EPS-AN8. Oral exposure to Cd lead to inevitable changes
in the microbiota structure followed by inflammation and oxidative stress of surrounding tissue, while
administration of EPS-AN8 reversed those changes.
PB  - Belgrade: Serbian Society of Toxicology
C3  - Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
T1  - Gut microbiota as a target of toxic compounds
T1  - Crevna mikrobiota kao osetljiva meta delovanja toksičnih supstanci
SP  - 92
EP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6113
ER  - 

@conference{
author = "Brdarić, Emilija and Popović, Dušanka and Kulaš, Jelena and Soković Bajić, Svetlana and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Crevna mikrobiota ima važnu ulogu u održavanju homeostaze koju ostvaruje preko interakcija sa imunskim
sistemom i gastrointestinalnim traktom. Toksične supstance ostvaruju direktan štetni efekat na sastav
i funkcionalnost crevne mikrobiote. Kadmijum (Cd) je metal sa izraženom toksičnošću, koji se povezuje
sa brojnim oboljenjima. U našoj studiji je predložen mehanizam zaštite koji bi se ostvario posredstvom
bakterijskih egzopolisaharida (EPS) izolovanih iz soja Lactiplantibacillus plantarum BGAN8, a bazira se
na visokom afinitetu adsorpcije kadmijumovih jona. Ciljevi rada su bili utvrđivanje efekata produženog
delovanja kadmijuma na sastav crevne mikrobiote Dark agouti (DA) pacova, kao i izučavanje efekta
EPS-AN8 na ublažavanje takvih štetnih efekata.
Pacovi su kroz vodu unosili nižu (5 ppm) i višu (50 ppm) dozu kadmijuma, dok je EPS-AN8 (100 μg/ml) dat
kroz hranu za životinje. Totalna DNK je izolovana iz lumena duodenuma i 16S rDNK amplikon je sekvenciran
strategijom uparenih krajeva na Illumina NovaSeq platformi. Dodatno, praćene su promene u
inflamaciji i oksidativnom stresu homogenata duodenuma. Kadmijum je favorizovao rast oportunističkih
patogenih bakterija poput pripadnika rodova Blautia, Prevotella, Alloprevotela, Bacteroides, dok je
relativna zastupljenost laktobacila bila značajno smanjena. Obe doze kadmijuma su stimulisale produkciju
proinflamatornih citokina (IL-1β, TNF-α i IFN-γ) i povisile nivo oksidativnog stresa (MDA, CAT i
GST). Oralni unos EPS-AN8 je značajno ublažio sve navedene promene. Oralna izloženost kadmijumu
uzrokuje nedvosmislene promene u sastavu mikrobiote, koje mogu biti praćene i pojačanim nivoima
inflamacije i oksidativnog stresa. Nasuprot tome, istovremeni unos EPS-AN8 redukuje pojavu takvih
promena., The gut microbiota has important role in maintaining homeostasis via its effect on the immune system
and healthy gastrointestinal tract. Environmental toxicants directly influenced the changes in gut microbiota
composition. Cadmium (Cd) is a hazard, world widely distributed metal. We have proposed
the putative mechanism of protection against Cd harmful effect in the organism by using exopolysaccharides
derived from Lactiplantibacillus plantarum BGAN8 (EPS-AN8) which has great ability to sequester Cd ions.
Aims of our study were to examine Cd related changes in the gut microbiota of Dark agouti (DA) rats after
prolonged oral exposure (30 days) to this metal and to determine impact of EPS-AN8 in preventing
those changes.
Rats were given via water lower (5 ppm) and higher (50 ppm) Cd’s dose. In paralel, EPS-AN8 was administrated
through food (100 μg/ml). Total DNA from duodenum was isolated and PCR amplicon for 16SrRNA was
sequenced on Illumina NovaSeq paired end platform. Moreover, we have followed changes in
oxidative stress and inflammation in duodenum. Cd favored growth of opportunistic pathogen bacteria
genera (Blautia, Prevotella, Alloprevotela, Bacteroides) while the relative abundance of lactobacilli was
decreased. The both doses of Cd increased production of inflammatory cytokines (IL-1β, TNF-α, and
IFN-γ). Also, parameters of oxidative stress were significantly upregulated (MDA, CAT, and GST).
All the mentioned changes were alleviated by EPS-AN8. Oral exposure to Cd lead to inevitable changes
in the microbiota structure followed by inflammation and oxidative stress of surrounding tissue, while
administration of EPS-AN8 reversed those changes.",
publisher = "Belgrade: Serbian Society of Toxicology",
journal = "Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia",
title = "Gut microbiota as a target of toxic compounds, Crevna mikrobiota kao osetljiva meta delovanja toksičnih supstanci",
pages = "92-93",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6113"
}

Brdarić, E., Popović, D., Kulaš, J., Soković Bajić, S., Golić, N., Mirkov, I.,& Živković, M.. (2023). Gut microbiota as a target of toxic compounds. in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
Belgrade: Serbian Society of Toxicology., 92-93.
https://hdl.handle.net/21.15107/rcub_ibiss_6113

Brdarić E, Popović D, Kulaš J, Soković Bajić S, Golić N, Mirkov I, Živković M. Gut microbiota as a target of toxic compounds. in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia. 2023;:92-93.
https://hdl.handle.net/21.15107/rcub_ibiss_6113 .

Brdarić, Emilija, Popović, Dušanka, Kulaš, Jelena, Soković Bajić, Svetlana, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Gut microbiota as a target of toxic compounds" in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia (2023):92-93,
https://hdl.handle.net/21.15107/rcub_ibiss_6113 .

TY  - CONF
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana
AU  - Tolinački, Maja
AU  - Popov Aleksandrov, Aleksandra
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6125
AB  - Кадмијум је глобално присутан токсични метал који изазива бројне штетне ефекте у организму и заузима седмо место на листи приоритетних супстанци од којих је неопходно пронаћи оптималне начине заштите. Наше пређашње студије су показале да егзополисахарид изолован из соја Lactiplantibacillus plantarum BGAN8 (EPS-AN8) показује висок афинитет за везивање јона кадмијума у воденом раствору и остварује значајан ниво in vitro заштите Caco-2 ћелија од његових токсичних ефеката. Имајући у виду да је за општу популацију најзаступљенији унос кадмијума исхраном, у овој студији је праћен паралелни ефекат уноса кадмијума (кроз воду) и EPS-AN8 (кроз храну) у DA пацовима. Након 30 дана третмана, утврђено је да је унос EPS-AN8 допринео сниженом нивоу депонованог метала у бубрезима, јетри и крви, а повећаном садржају у фекалном материјалу. Такође, микрографије дуоденума, бубрега и јетре су указале на ниже степене кадмијумом индукованих оштећења. Праћењем активности ензима који учествују у антиоксидативној заштити (CAT, GST), терминалних производа липидне пероксидације (MDA) и продукције проинфламаторних цитокина (IL-1β, TNFα, IFN-γ) у хомогенатима дуоденума, утврђен је нижи ниво оксидативног стреса и инфламације, што представља додатни показатељ заштите коју остварује EPS-AN8. С обзиром да орални унос кадмијума доводи до значајних промена у цревној микробиоти, изоловали смо тоталну DNK из дуоденума и секвенцирали 16S rDNK ампликон на Illumina NovaSeq платформи. Примећено је да унос EPS-AN8 ублажава појаву промена у релативној заступљености бактеријских родова и врста карактерисичних за излагање кадмијуму, попут пораста броја опортунистичких патогених бактерија и смањења бројности лактобацила. Остварени заштитни ефекти указују на снажан потенцијал примене EPS-AN8 у условима високе контаминације кадмијумом.
AB  - Kadmijum je globalno prisutan toksični metal koji izaziva brojne štetne efekte u organizmu i zauzima sedmo mesto na listi prioritetnih supstanci od kojih je neophodno pronaći optimalne načine zaštite. Naše pređašnje studije su pokazale da egzopolisaharid izolovan iz soja Lactiplantibacillus plantarum BGAN8 (EPS-AN8) pokazuje visok afinitet za vezivanje jona kadmijuma u vodenom rastvoru i ostvaruje značajan nivo in vitro zaštite Caco-2 ćelija od njegovih toksičnih efekata. Imajući u vidu da je za opštu populaciju najzastupljeniji unos kadmijuma ishranom, u ovoj studiji je praćen paralelni efekat unosa kadmijuma (kroz vodu) i EPS-AN8 (kroz hranu) u DA pacovima. Nakon 30 dana tretmana, utvrđeno je da je unos EPS-AN8 doprineo sniženom nivou deponovanog metala u bubrezima, jetri i krvi, a povećanom sadržaju u fekalnom materijalu. Takođe, mikrografije duodenuma, bubrega i jetre su ukazale na niže stepene kadmijumom indukovanih oštećenja. Praćenjem aktivnosti enzima koji učestvuju u antioksidativnoj zaštiti (CAT, GST), terminalnih proizvoda lipidne peroksidacije (MDA) i produkcije proinflamatornih citokina (IL-1β, TNFα, IFN-γ) u homogenatima duodenuma, utvrđen je niži nivo oksidativnog stresa i inflamacije, što predstavlja dodatni pokazatelj zaštite koju ostvaruje EPS-AN8. S obzirom da oralni unos kadmijuma dovodi do značajnih promena u crevnoj mikrobioti, izolovali smo totalnu DNK iz duodenuma i sekvencirali 16S rDNK amplikon na Illumina NovaSeq platformi. Primećeno je da unos EPS-AN8 ublažava pojavu promena u relativnoj zastupljenosti bakterijskih rodova i vrsta karakterističnih za ilaganje kadmijumu, poput porasta broja oportunističkih patogenih bakterija i smanjenja brojnosti laktobacila. Ostvareni zaštitni efekti ukazuju na snažan potencijal primene EPS-AN8 u uslovima visoke kontaminacije kadmijumom.
PB  - Belgrade: Serbian Academy of Sciences and Arts
C3  - Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
T1  - Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II)
T1  - Uloga EPS-AN8 u zaštiti DA pacova izloženih oralnom unosu kadmijuma(II)
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6125
ER  - 

@conference{
author = "Brdarić, Emilija and Popović, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana and Tolinački, Maja and Popov Aleksandrov, Aleksandra and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Кадмијум је глобално присутан токсични метал који изазива бројне штетне ефекте у организму и заузима седмо место на листи приоритетних супстанци од којих је неопходно пронаћи оптималне начине заштите. Наше пређашње студије су показале да егзополисахарид изолован из соја Lactiplantibacillus plantarum BGAN8 (EPS-AN8) показује висок афинитет за везивање јона кадмијума у воденом раствору и остварује значајан ниво in vitro заштите Caco-2 ћелија од његових токсичних ефеката. Имајући у виду да је за општу популацију најзаступљенији унос кадмијума исхраном, у овој студији је праћен паралелни ефекат уноса кадмијума (кроз воду) и EPS-AN8 (кроз храну) у DA пацовима. Након 30 дана третмана, утврђено је да је унос EPS-AN8 допринео сниженом нивоу депонованог метала у бубрезима, јетри и крви, а повећаном садржају у фекалном материјалу. Такође, микрографије дуоденума, бубрега и јетре су указале на ниже степене кадмијумом индукованих оштећења. Праћењем активности ензима који учествују у антиоксидативној заштити (CAT, GST), терминалних производа липидне пероксидације (MDA) и продукције проинфламаторних цитокина (IL-1β, TNFα, IFN-γ) у хомогенатима дуоденума, утврђен је нижи ниво оксидативног стреса и инфламације, што представља додатни показатељ заштите коју остварује EPS-AN8. С обзиром да орални унос кадмијума доводи до значајних промена у цревној микробиоти, изоловали смо тоталну DNK из дуоденума и секвенцирали 16S rDNK ампликон на Illumina NovaSeq платформи. Примећено је да унос EPS-AN8 ублажава појаву промена у релативној заступљености бактеријских родова и врста карактерисичних за излагање кадмијуму, попут пораста броја опортунистичких патогених бактерија и смањења бројности лактобацила. Остварени заштитни ефекти указују на снажан потенцијал примене EPS-AN8 у условима високе контаминације кадмијумом., Kadmijum je globalno prisutan toksični metal koji izaziva brojne štetne efekte u organizmu i zauzima sedmo mesto na listi prioritetnih supstanci od kojih je neophodno pronaći optimalne načine zaštite. Naše pređašnje studije su pokazale da egzopolisaharid izolovan iz soja Lactiplantibacillus plantarum BGAN8 (EPS-AN8) pokazuje visok afinitet za vezivanje jona kadmijuma u vodenom rastvoru i ostvaruje značajan nivo in vitro zaštite Caco-2 ćelija od njegovih toksičnih efekata. Imajući u vidu da je za opštu populaciju najzastupljeniji unos kadmijuma ishranom, u ovoj studiji je praćen paralelni efekat unosa kadmijuma (kroz vodu) i EPS-AN8 (kroz hranu) u DA pacovima. Nakon 30 dana tretmana, utvrđeno je da je unos EPS-AN8 doprineo sniženom nivou deponovanog metala u bubrezima, jetri i krvi, a povećanom sadržaju u fekalnom materijalu. Takođe, mikrografije duodenuma, bubrega i jetre su ukazale na niže stepene kadmijumom indukovanih oštećenja. Praćenjem aktivnosti enzima koji učestvuju u antioksidativnoj zaštiti (CAT, GST), terminalnih proizvoda lipidne peroksidacije (MDA) i produkcije proinflamatornih citokina (IL-1β, TNFα, IFN-γ) u homogenatima duodenuma, utvrđen je niži nivo oksidativnog stresa i inflamacije, što predstavlja dodatni pokazatelj zaštite koju ostvaruje EPS-AN8. S obzirom da oralni unos kadmijuma dovodi do značajnih promena u crevnoj mikrobioti, izolovali smo totalnu DNK iz duodenuma i sekvencirali 16S rDNK amplikon na Illumina NovaSeq platformi. Primećeno je da unos EPS-AN8 ublažava pojavu promena u relativnoj zastupljenosti bakterijskih rodova i vrsta karakterističnih za ilaganje kadmijumu, poput porasta broja oportunističkih patogenih bakterija i smanjenja brojnosti laktobacila. Ostvareni zaštitni efekti ukazuju na snažan potencijal primene EPS-AN8 u uslovima visoke kontaminacije kadmijumom.",
publisher = "Belgrade: Serbian Academy of Sciences and Arts",
journal = "Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia",
title = "Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II), Uloga EPS-AN8 u zaštiti DA pacova izloženih oralnom unosu kadmijuma(II)",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6125"
}

Brdarić, E., Popović, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S., Tolinački, M., Popov Aleksandrov, A., Golić, N., Mirkov, I.,& Živković, M.. (2023). Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II). in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia
Belgrade: Serbian Academy of Sciences and Arts..
https://hdl.handle.net/21.15107/rcub_ibiss_6125

Brdarić E, Popović D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić S, Tolinački M, Popov Aleksandrov A, Golić N, Mirkov I, Živković M. Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II). in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6125 .

Brdarić, Emilija, Popović, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana, Tolinački, Maja, Popov Aleksandrov, Aleksandra, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Улога EPS-AN8 у заштити DA пацова изложених оралном уносу кадмијума(II)" in Naučni skup Svetski dan imunologije 2023; 2023 Apr 27; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6125 .

TY  - JOUR
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana
AU  - Tolinački, Maja
AU  - Popov Aleksandrov, Aleksandra
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5479
AB  - Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is
correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS)
derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for
the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food
supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty
days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led
to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas
the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on
the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation
(IL-1 , TNF- , and IFN-
) in the duodenum. Moreover, micrographs of the duodenum were in line
with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used
16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the
microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly
favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those
changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting
this toxic environmental pollutant.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats
IS  - 3
VL  - 24
DO  - 10.3390/ijms24032845
SP  - 2845
ER  - 

@article{
author = "Brdarić, Emilija and Popović, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana and Tolinački, Maja and Popov Aleksandrov, Aleksandra and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is
correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS)
derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for
the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food
supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty
days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led
to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas
the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on
the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation
(IL-1 , TNF- , and IFN-
) in the duodenum. Moreover, micrographs of the duodenum were in line
with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used
16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the
microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly
favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those
changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting
this toxic environmental pollutant.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats",
number = "3",
volume = "24",
doi = "10.3390/ijms24032845",
pages = "2845"
}

Brdarić, E., Popović, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S., Tolinački, M., Popov Aleksandrov, A., Golić, N., Mirkov, I.,& Živković, M.. (2023). Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences
Basel: MDPI., 24(3), 2845.
https://doi.org/10.3390/ijms24032845

Brdarić E, Popović D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić S, Tolinački M, Popov Aleksandrov A, Golić N, Mirkov I, Živković M. Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences. 2023;24(3):2845.
doi:10.3390/ijms24032845 .

Brdarić, Emilija, Popović, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana, Tolinački, Maja, Popov Aleksandrov, Aleksandra, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats" in International Journal of Molecular Sciences, 24, no. 3 (2023):2845,
https://doi.org/10.3390/ijms24032845 . .

TY  - CONF
AU  - Živković, Milica
AU  - Soković Bajić, Svetlana
AU  - Tolinački, Maja
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Golić, Nataša
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5690
AB  - Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.
PB  - Wolters Kluwer Health
C3  - 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
T1  - The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11
DO  - 10.1097/MCG.0000000000001292
SP  - 103
ER  - 

@conference{
author = "Živković, Milica and Soković Bajić, Svetlana and Tolinački, Maja and Brdarić, Emilija and Đokić, Jelena and Popović, Nikola and Rajić, Jovana and Đorđević, Marija and Golić, Nataša",
year = "2019",
abstract = "Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.",
publisher = "Wolters Kluwer Health",
journal = "10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy",
title = "The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11",
doi = "10.1097/MCG.0000000000001292",
pages = "103"
}

Živković, M., Soković Bajić, S., Tolinački, M., Brdarić, E., Đokić, J., Popović, N., Rajić, J., Đorđević, M.,& Golić, N.. (2019). The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
Wolters Kluwer Health., 103.
https://doi.org/10.1097/MCG.0000000000001292

Živković M, Soković Bajić S, Tolinački M, Brdarić E, Đokić J, Popović N, Rajić J, Đorđević M, Golić N. The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. 2019;:103.
doi:10.1097/MCG.0000000000001292 .

Živković, Milica, Soković Bajić, Svetlana, Tolinački, Maja, Brdarić, Emilija, Đokić, Jelena, Popović, Nikola, Rajić, Jovana, Đorđević, Marija, Golić, Nataša, "The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11" in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy (2019):103,
https://doi.org/10.1097/MCG.0000000000001292 . .

TY  - JOUR
AU  - Velimirović, Milica
AU  - Jevtić Dožudić, Gordana
AU  - Selaković, Vesna
AU  - Stojković, Tihomir
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Živković, Milica
AU  - Dragutinović, Vesna
AU  - Nikolić, Tatjana
AU  - Jelenković, Ankica
AU  - Đorović, Đorđe
AU  - Mirčić, Aleksandar
AU  - Petronijević, Nataša D.
PY  - 2018
UR  - https://www.hindawi.com/journals/omcl/2018/3273654/
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5932460
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3167
AB  - Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.
T2  - Oxidative medicine and cellular longevity
T1  - Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.
VL  - 2018
DO  - 10.1155/2018/3273654
SP  - 3273654
ER  - 

@article{
author = "Velimirović, Milica and Jevtić Dožudić, Gordana and Selaković, Vesna and Stojković, Tihomir and Puškaš, Nela and Zaletel, Ivan and Živković, Milica and Dragutinović, Vesna and Nikolić, Tatjana and Jelenković, Ankica and Đorović, Đorđe and Mirčić, Aleksandar and Petronijević, Nataša D.",
year = "2018",
abstract = "Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.",
journal = "Oxidative medicine and cellular longevity",
title = "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.",
volume = "2018",
doi = "10.1155/2018/3273654",
pages = "3273654"
}

Velimirović, M., Jevtić Dožudić, G., Selaković, V., Stojković, T., Puškaš, N., Zaletel, I., Živković, M., Dragutinović, V., Nikolić, T., Jelenković, A., Đorović, Đ., Mirčić, A.,& Petronijević, N. D.. (2018). Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.. in Oxidative medicine and cellular longevity, 2018, 3273654.
https://doi.org/10.1155/2018/3273654

Velimirović M, Jevtić Dožudić G, Selaković V, Stojković T, Puškaš N, Zaletel I, Živković M, Dragutinović V, Nikolić T, Jelenković A, Đorović Đ, Mirčić A, Petronijević ND. Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.. in Oxidative medicine and cellular longevity. 2018;2018:3273654.
doi:10.1155/2018/3273654 .

Velimirović, Milica, Jevtić Dožudić, Gordana, Selaković, Vesna, Stojković, Tihomir, Puškaš, Nela, Zaletel, Ivan, Živković, Milica, Dragutinović, Vesna, Nikolić, Tatjana, Jelenković, Ankica, Đorović, Đorđe, Mirčić, Aleksandar, Petronijević, Nataša D., "Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia." in Oxidative medicine and cellular longevity, 2018 (2018):3273654,
https://doi.org/10.1155/2018/3273654 . .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Živković, Milica
AU  - Arambašić Jovanović, Jelena
AU  - Tolinački, Maja
AU  - Đorđević, Marija
AU  - Rajić, Jovana
AU  - Uskoković, Aleksandra
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Golić, Nataša
AU  - Vidaković, Melita
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464617305546
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2862
AB  - The aim of this study was to assess the effect of the probiotic Lactobacillus paraplantarum BGCG11 on the regulatory pathways that underlie the defense responses of the liver and kidney in diabetic rats. Probiotic-treated diabetic rats exhibited decreased hyperglycemia, glycated hemoglobin, triacylglycerols and a reduction in advanced glycation end products of serum proteins. The probiotic treatment adjusted the redox imbalance in the liver and kidney of diabetic rats, reduced the level of DNA damage, increased the activity of the pro-survival Akt kinase, decreased procaspase 3 degradation and lowered the level of inflammatory mediator C/EBPβ. Administration of probiotic to diabetic rats attenuated fibrotic process activated in the liver and kidneys as judged by the increase in E-cadherin and decreases in α-smooth muscle actin and fibronectin. In summary, the probiotic administration had an ameliorating effect on diabetes-associated disturbed redox homeostasis, inflammation and fibrosis, which underline the development of diabetic complications.
T2  - Journal of Functional Foods
T1  - Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats
IS  - Part A
VL  - 38
DO  - 10.1016/j.jff.2017.09.033
SP  - 427
EP  - 437
ER  - 

@article{
author = "Mihailović, Mirjana and Živković, Milica and Arambašić Jovanović, Jelena and Tolinački, Maja and Đorđević, Marija and Rajić, Jovana and Uskoković, Aleksandra and Dinić, Svetlana and Grdović, Nevena and Golić, Nataša and Vidaković, Melita",
year = "2017",
abstract = "The aim of this study was to assess the effect of the probiotic Lactobacillus paraplantarum BGCG11 on the regulatory pathways that underlie the defense responses of the liver and kidney in diabetic rats. Probiotic-treated diabetic rats exhibited decreased hyperglycemia, glycated hemoglobin, triacylglycerols and a reduction in advanced glycation end products of serum proteins. The probiotic treatment adjusted the redox imbalance in the liver and kidney of diabetic rats, reduced the level of DNA damage, increased the activity of the pro-survival Akt kinase, decreased procaspase 3 degradation and lowered the level of inflammatory mediator C/EBPβ. Administration of probiotic to diabetic rats attenuated fibrotic process activated in the liver and kidneys as judged by the increase in E-cadherin and decreases in α-smooth muscle actin and fibronectin. In summary, the probiotic administration had an ameliorating effect on diabetes-associated disturbed redox homeostasis, inflammation and fibrosis, which underline the development of diabetic complications.",
journal = "Journal of Functional Foods",
title = "Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats",
number = "Part A",
volume = "38",
doi = "10.1016/j.jff.2017.09.033",
pages = "427-437"
}

Mihailović, M., Živković, M., Arambašić Jovanović, J., Tolinački, M., Đorđević, M., Rajić, J., Uskoković, A., Dinić, S., Grdović, N., Golić, N.,& Vidaković, M.. (2017). Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats. in Journal of Functional Foods, 38(Part A), 427-437.
https://doi.org/10.1016/j.jff.2017.09.033

Mihailović M, Živković M, Arambašić Jovanović J, Tolinački M, Đorđević M, Rajić J, Uskoković A, Dinić S, Grdović N, Golić N, Vidaković M. Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats. in Journal of Functional Foods. 2017;38(Part A):427-437.
doi:10.1016/j.jff.2017.09.033 .

Mihailović, Mirjana, Živković, Milica, Arambašić Jovanović, Jelena, Tolinački, Maja, Đorđević, Marija, Rajić, Jovana, Uskoković, Aleksandra, Dinić, Svetlana, Grdović, Nevena, Golić, Nataša, Vidaković, Melita, "Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats" in Journal of Functional Foods, 38, no. Part A (2017):427-437,
https://doi.org/10.1016/j.jff.2017.09.033 . .