TY  - JOUR
AU  - Bogdanović, Milica
AU  - Dragičević, Milan B.
AU  - Tanić, Nikola T
AU  - Todorović, Slađana
AU  - Mišić, Danijela
AU  - Živković, Suzana
AU  - Tissier, Alain
AU  - Simonović, Ana
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1045
AB  - Ribosomal 18S RNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern analysis, and real-time experiments. However, there are two disadvantages and two points of error introduction in using 18S rRNA as a reference gene. First, 18S has no poly(A) tail, so it is commonly reverse transcribed with specific primers or random hexamers, independently from poly(dT)-primed transcripts. Secondly, due to its abundance, the 18S cDNA must be extensively diluted to be comparable to the tested genes. In this study, 18S rRNA from five taxonomically diverse plant species, including Physcomitrella patens, Adiantum capillus-veneris, Centaurium erythraea, Arabidopsis thaliana, and Zea mays, was successfully reverse transcribed (RT) using poly(dT)(18). As all other homopolymers, including poly(dA)(18), poly(dC)(18), and poly(dG)(18), could serve as RT primers, it was concluded that homopolymers anneal by mispriming at the sites of complementary homopolymeric runs or segments rich in complementary base. Poly(dC)(18) was the most efficient as RT primer, and the only one which interfered with subsequent PCR, giving species-specific pattern of products. Poly(dT)-primed RT reactions were less efficient in comparison to specific primer or random hexamer-primed reactions. Homopolymeric priming of 18S in RT reactions is general in terms of RNA origin and the method of RNA isolation and is possibly applicable to other tailless housekeeping genes.
T2  - Plant Molecular Biology Reporter
T1  - Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers
IS  - 1
VL  - 31
SP  - 1
EP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1045
ER  - 

@article{
author = "Bogdanović, Milica and Dragičević, Milan B. and Tanić, Nikola T and Todorović, Slađana and Mišić, Danijela and Živković, Suzana and Tissier, Alain and Simonović, Ana",
year = "2013",
abstract = "Ribosomal 18S RNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern analysis, and real-time experiments. However, there are two disadvantages and two points of error introduction in using 18S rRNA as a reference gene. First, 18S has no poly(A) tail, so it is commonly reverse transcribed with specific primers or random hexamers, independently from poly(dT)-primed transcripts. Secondly, due to its abundance, the 18S cDNA must be extensively diluted to be comparable to the tested genes. In this study, 18S rRNA from five taxonomically diverse plant species, including Physcomitrella patens, Adiantum capillus-veneris, Centaurium erythraea, Arabidopsis thaliana, and Zea mays, was successfully reverse transcribed (RT) using poly(dT)(18). As all other homopolymers, including poly(dA)(18), poly(dC)(18), and poly(dG)(18), could serve as RT primers, it was concluded that homopolymers anneal by mispriming at the sites of complementary homopolymeric runs or segments rich in complementary base. Poly(dC)(18) was the most efficient as RT primer, and the only one which interfered with subsequent PCR, giving species-specific pattern of products. Poly(dT)-primed RT reactions were less efficient in comparison to specific primer or random hexamer-primed reactions. Homopolymeric priming of 18S in RT reactions is general in terms of RNA origin and the method of RNA isolation and is possibly applicable to other tailless housekeeping genes.",
journal = "Plant Molecular Biology Reporter",
title = "Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers",
number = "1",
volume = "31",
pages = "1-63",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1045"
}

Bogdanović, M., Dragičević, M. B., Tanić, N. T., Todorović, S., Mišić, D., Živković, S., Tissier, A.,& Simonović, A.. (2013). Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers. in Plant Molecular Biology Reporter, 31(1), 1-63.
https://hdl.handle.net/21.15107/rcub_ibiss_1045

Bogdanović M, Dragičević MB, Tanić NT, Todorović S, Mišić D, Živković S, Tissier A, Simonović A. Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers. in Plant Molecular Biology Reporter. 2013;31(1):1-63.
https://hdl.handle.net/21.15107/rcub_ibiss_1045 .

Bogdanović, Milica, Dragičević, Milan B., Tanić, Nikola T, Todorović, Slađana, Mišić, Danijela, Živković, Suzana, Tissier, Alain, Simonović, Ana, "Reverse Transcription of 18S rRNA with Poly(dT)(18) and Other Homopolymers" in Plant Molecular Biology Reporter, 31, no. 1 (2013):1-63,
https://hdl.handle.net/21.15107/rcub_ibiss_1045 .

TY  - JOUR
AU  - Dragičević, Milan B.
AU  - Platiša, Jelena
AU  - Nikolić, Radomirka R
AU  - Todorović, Slađana
AU  - Bogdanović, Milica
AU  - Banjac, Nevena
AU  - Simonović, Ana
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1052
AB  - Herbicide phosphinothricin (PPT) inhibits glutamine synthetase (GS), a key enzyme in nitrogen assimilation, thus causing ammonia accumulation, glutamine depletion and eventually plant death. However, the growth response of Lotus corniculatus L. plants immersed in solutions with a broad range of PPT concentrations is biphasic, with pronounced stimulating effect on biomass production at concentrations <= 50 mu M and growth inhibition at higher concentrations. The growth stimulation at low PPT concentrations is a result of activation of chloroplastic isoform GS2, while the growth suppression is caused by inhibition of both cytosolic GS1 and GS2 at higher PPT concentrations. Since the results are obtained in cell-free system (e. g. protein extracts), to which the principles of homeostasis are not applicable, this PPT effect is an unambiguous example of direct stimulation hormesis. A detailed molecular mechanism of concentration-dependent interaction of both PPT and a related GS inhibitor, methionine sulfoximine, with GS holoenzymes is proposed. The mechanism is in concurrence with all experimental and literature data.
T2  - Dose-Response
T1  - Herbicide Phosphinothricin Causes Direct Stimulation Hormesis
IS  - 3
VL  - 11
SP  - 299
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1052
ER  - 

@article{
author = "Dragičević, Milan B. and Platiša, Jelena and Nikolić, Radomirka R and Todorović, Slađana and Bogdanović, Milica and Banjac, Nevena and Simonović, Ana",
year = "2013",
abstract = "Herbicide phosphinothricin (PPT) inhibits glutamine synthetase (GS), a key enzyme in nitrogen assimilation, thus causing ammonia accumulation, glutamine depletion and eventually plant death. However, the growth response of Lotus corniculatus L. plants immersed in solutions with a broad range of PPT concentrations is biphasic, with pronounced stimulating effect on biomass production at concentrations <= 50 mu M and growth inhibition at higher concentrations. The growth stimulation at low PPT concentrations is a result of activation of chloroplastic isoform GS2, while the growth suppression is caused by inhibition of both cytosolic GS1 and GS2 at higher PPT concentrations. Since the results are obtained in cell-free system (e. g. protein extracts), to which the principles of homeostasis are not applicable, this PPT effect is an unambiguous example of direct stimulation hormesis. A detailed molecular mechanism of concentration-dependent interaction of both PPT and a related GS inhibitor, methionine sulfoximine, with GS holoenzymes is proposed. The mechanism is in concurrence with all experimental and literature data.",
journal = "Dose-Response",
title = "Herbicide Phosphinothricin Causes Direct Stimulation Hormesis",
number = "3",
volume = "11",
pages = "299-360",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1052"
}

Dragičević, M. B., Platiša, J., Nikolić, R. R., Todorović, S., Bogdanović, M., Banjac, N.,& Simonović, A.. (2013). Herbicide Phosphinothricin Causes Direct Stimulation Hormesis. in Dose-Response, 11(3), 299-360.
https://hdl.handle.net/21.15107/rcub_ibiss_1052

Dragičević MB, Platiša J, Nikolić RR, Todorović S, Bogdanović M, Banjac N, Simonović A. Herbicide Phosphinothricin Causes Direct Stimulation Hormesis. in Dose-Response. 2013;11(3):299-360.
https://hdl.handle.net/21.15107/rcub_ibiss_1052 .

Dragičević, Milan B., Platiša, Jelena, Nikolić, Radomirka R, Todorović, Slađana, Bogdanović, Milica, Banjac, Nevena, Simonović, Ana, "Herbicide Phosphinothricin Causes Direct Stimulation Hormesis" in Dose-Response, 11, no. 3 (2013):299-360,
https://hdl.handle.net/21.15107/rcub_ibiss_1052 .

TY  - JOUR
AU  - Petrić, Marija P
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Tadić, Vojin
AU  - Milošević, Snežana
AU  - Dragičević, Milan B.
AU  - Subotić, Angelina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/956
AB  - We investigated the effect of low temperature and gibberellic acid (GA(3)) treatment on dormancy in Fritillaria meleagris L. bulbs. Also, we studied the effect of dormancy breaking on the antioxidant enzymes activity. To overcome dormancy, bulbs require a period (4-8 weeks) of exposure to low temperature. Bulbs regenerated in vitro were grown in the dark on medium without growth regulators at the standard (24 A degrees C) or at low temperatures (4 and 15 A degrees C) for 4, 6, 8 and 10 weeks. Bulbs were collected after 3, 4 and 5 weeks of cooling at 4 A degrees C. To investigate the influence of GA(3) on dormancy, bulbs were treated for 24 h with GA(3) solutions with 1, 2 and 3 mg l(-1) concentrations. During the period of growth of bulbs at 4 A degrees C, regeneration of bulbs was very weak, while at 15 A degrees C the number of regenerated bulbs increased significantly. Improved bulb sprouting was achieved by a short treatment with gibberellin. Low temperature also represents a kind of oxidative stress for the plant. The activity of superoxide dismutase, catalase (CAT) and peroxidase (POX) in bulbs of F. meleagris L. grown in vitro and ex vitro increased with decreasing temperature in contrast to glutathione reductase. POX showed generally lower activity than CAT which indicates that major role in the breaking dormancy and preparing bulbs for sprouting have catalases.
T2  - Acta Physiologiae Plantarum
T1  - The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro
IS  - 11
VL  - 35
SP  - 23
EP  - 3236
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_956
ER  - 

@article{
author = "Petrić, Marija P and Jevremović, Slađana and Trifunović-Momčilov, Milana and Tadić, Vojin and Milošević, Snežana and Dragičević, Milan B. and Subotić, Angelina",
year = "2013",
abstract = "We investigated the effect of low temperature and gibberellic acid (GA(3)) treatment on dormancy in Fritillaria meleagris L. bulbs. Also, we studied the effect of dormancy breaking on the antioxidant enzymes activity. To overcome dormancy, bulbs require a period (4-8 weeks) of exposure to low temperature. Bulbs regenerated in vitro were grown in the dark on medium without growth regulators at the standard (24 A degrees C) or at low temperatures (4 and 15 A degrees C) for 4, 6, 8 and 10 weeks. Bulbs were collected after 3, 4 and 5 weeks of cooling at 4 A degrees C. To investigate the influence of GA(3) on dormancy, bulbs were treated for 24 h with GA(3) solutions with 1, 2 and 3 mg l(-1) concentrations. During the period of growth of bulbs at 4 A degrees C, regeneration of bulbs was very weak, while at 15 A degrees C the number of regenerated bulbs increased significantly. Improved bulb sprouting was achieved by a short treatment with gibberellin. Low temperature also represents a kind of oxidative stress for the plant. The activity of superoxide dismutase, catalase (CAT) and peroxidase (POX) in bulbs of F. meleagris L. grown in vitro and ex vitro increased with decreasing temperature in contrast to glutathione reductase. POX showed generally lower activity than CAT which indicates that major role in the breaking dormancy and preparing bulbs for sprouting have catalases.",
journal = "Acta Physiologiae Plantarum",
title = "The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro",
number = "11",
volume = "35",
pages = "23-3236",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_956"
}

Petrić, M. P., Jevremović, S., Trifunović-Momčilov, M., Tadić, V., Milošević, S., Dragičević, M. B.,& Subotić, A.. (2013). The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro. in Acta Physiologiae Plantarum, 35(11), 23-3236.
https://hdl.handle.net/21.15107/rcub_ibiss_956

Petrić MP, Jevremović S, Trifunović-Momčilov M, Tadić V, Milošević S, Dragičević MB, Subotić A. The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro. in Acta Physiologiae Plantarum. 2013;35(11):23-3236.
https://hdl.handle.net/21.15107/rcub_ibiss_956 .

Petrić, Marija P, Jevremović, Slađana, Trifunović-Momčilov, Milana, Tadić, Vojin, Milošević, Snežana, Dragičević, Milan B., Subotić, Angelina, "The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro" in Acta Physiologiae Plantarum, 35, no. 11 (2013):23-3236,
https://hdl.handle.net/21.15107/rcub_ibiss_956 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Dragičević, Milan B.
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Maksimović, Vuk M
AU  - Momčilović, Ivana
AU  - Nikolić, Miroslav
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1110
AB  - A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
T2  - Journal of Plant Physiology
T1  - Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
IS  - 13
VL  - 169
SP  - 93
EP  - 1289
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1110
ER  - 

@article{
author = "Mišić, Danijela and Dragičević, Milan B. and Šiler, Branislav and Nestorović Živković, Jasmina and Maksimović, Vuk M and Momčilović, Ivana and Nikolić, Miroslav",
year = "2012",
abstract = "A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.",
journal = "Journal of Plant Physiology",
title = "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress",
number = "13",
volume = "169",
pages = "93-1289",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1110"
}

Mišić, D., Dragičević, M. B., Šiler, B., Nestorović Živković, J., Maksimović, V. M., Momčilović, I.,& Nikolić, M.. (2012). Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology, 169(13), 93-1289.
https://hdl.handle.net/21.15107/rcub_ibiss_1110

Mišić D, Dragičević MB, Šiler B, Nestorović Živković J, Maksimović VM, Momčilović I, Nikolić M. Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology. 2012;169(13):93-1289.
https://hdl.handle.net/21.15107/rcub_ibiss_1110 .

Mišić, Danijela, Dragičević, Milan B., Šiler, Branislav, Nestorović Živković, Jasmina, Maksimović, Vuk M, Momčilović, Ivana, Nikolić, Miroslav, "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress" in Journal of Plant Physiology, 169, no. 13 (2012):93-1289,
https://hdl.handle.net/21.15107/rcub_ibiss_1110 .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Božić, Dragana
AU  - Simonović, Ana
AU  - Filipović, Biljana
AU  - Dragičević, Milan B.
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1353
AB  - The Empress tree (Paulownia tomentosa Steud.) is a fast growing deciduous tree originating from East Asia. It is grown as an ornamental plant, but can also be used as industrial wood. However, in some parts of North America, this species is considered invasive, with high impact on autochthonous populations. We have investigated whether post-fire expansion of this species can be explained at the germination level by analyzing the interaction between physical (light and temperature) and chemical (smoke and nitrogenous compounds) fire-related cues in the regulation of P. tomentosa germination. Light is the key environmental signal in the induction of germination of positively photoblastic P. tomentosa seeds. Smoke, applied in the form of extract (liquid smoke [LS]), stimulates light-induced germination if applied during imbibition, or shortly after the inductive irradiation. This stimulatory effect of LS can be effectively prevented by far-red light, suggesting that the mechanism of smoke action is phytochrome related. The LS had no effect on the germination percentage when applied during the phase of radicle protrusion. However, LS reduced radicle elongation and slowed its protrusion. The LS had no effect on the optimal temperature range of germination (23-27 degrees C). The combined action of LS and KNO(3) in light-induced germination was more effective then when each chemical was applied separately. It can be concluded that all tested components of fire, except elevated temperature, operate to stimulate germination of P. tomentosa seeds, suggesting that the post-fire invasive potential of this species can, at least in part, be explained at the germination level.
T2  - Plant Species Biology
T1  - Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud
IS  - 3
VL  - 25
EP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1353
ER  - 

@article{
author = "Todorović, Slađana and Božić, Dragana and Simonović, Ana and Filipović, Biljana and Dragičević, Milan B. and Giba, Zlatko and Grubišić, Dragoljub",
year = "2010",
abstract = "The Empress tree (Paulownia tomentosa Steud.) is a fast growing deciduous tree originating from East Asia. It is grown as an ornamental plant, but can also be used as industrial wood. However, in some parts of North America, this species is considered invasive, with high impact on autochthonous populations. We have investigated whether post-fire expansion of this species can be explained at the germination level by analyzing the interaction between physical (light and temperature) and chemical (smoke and nitrogenous compounds) fire-related cues in the regulation of P. tomentosa germination. Light is the key environmental signal in the induction of germination of positively photoblastic P. tomentosa seeds. Smoke, applied in the form of extract (liquid smoke [LS]), stimulates light-induced germination if applied during imbibition, or shortly after the inductive irradiation. This stimulatory effect of LS can be effectively prevented by far-red light, suggesting that the mechanism of smoke action is phytochrome related. The LS had no effect on the germination percentage when applied during the phase of radicle protrusion. However, LS reduced radicle elongation and slowed its protrusion. The LS had no effect on the optimal temperature range of germination (23-27 degrees C). The combined action of LS and KNO(3) in light-induced germination was more effective then when each chemical was applied separately. It can be concluded that all tested components of fire, except elevated temperature, operate to stimulate germination of P. tomentosa seeds, suggesting that the post-fire invasive potential of this species can, at least in part, be explained at the germination level.",
journal = "Plant Species Biology",
title = "Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud",
number = "3",
volume = "25",
pages = "202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1353"
}

Todorović, S., Božić, D., Simonović, A., Filipović, B., Dragičević, M. B., Giba, Z.,& Grubišić, D.. (2010). Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud. in Plant Species Biology, 25(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1353

Todorović S, Božić D, Simonović A, Filipović B, Dragičević MB, Giba Z, Grubišić D. Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud. in Plant Species Biology. 2010;25(3):null-202.
https://hdl.handle.net/21.15107/rcub_ibiss_1353 .

Todorović, Slađana, Božić, Dragana, Simonović, Ana, Filipović, Biljana, Dragičević, Milan B., Giba, Zlatko, Grubišić, Dragoljub, "Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud" in Plant Species Biology, 25, no. 3 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1353 .