TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - Aquilanti, Giuliana
AU  - Cvetić Antić, Tijana
AU  - Todorović, Nataša
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5733
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.
PB  - Belgrade: Society of Physical Chemists of Serbia
C3  - Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 1; 2022 Sep 26-30; Belgrade, Serbia
T1  - Selenite metabolism in the mycelium of the fungus Phycomyces blakesleeanus
SP  - 93
EP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5733
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and Aquilanti, Giuliana and Cvetić Antić, Tijana and Todorović, Nataša and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.",
publisher = "Belgrade: Society of Physical Chemists of Serbia",
journal = "Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 1; 2022 Sep 26-30; Belgrade, Serbia",
title = "Selenite metabolism in the mycelium of the fungus Phycomyces blakesleeanus",
pages = "93-96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5733"
}

Žižić, M., Stanić, M., Aquilanti, G., Cvetić Antić, T., Todorović, N., Živić, M.,& Zakrzewska, J.. (2022). Selenite metabolism in the mycelium of the fungus Phycomyces blakesleeanus. in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 1; 2022 Sep 26-30; Belgrade, Serbia
Belgrade: Society of Physical Chemists of Serbia., 93-96.
https://hdl.handle.net/21.15107/rcub_ibiss_5733

Žižić M, Stanić M, Aquilanti G, Cvetić Antić T, Todorović N, Živić M, Zakrzewska J. Selenite metabolism in the mycelium of the fungus Phycomyces blakesleeanus. in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 1; 2022 Sep 26-30; Belgrade, Serbia. 2022;:93-96.
https://hdl.handle.net/21.15107/rcub_ibiss_5733 .

Žižić, Milan, Stanić, Marina, Aquilanti, Giuliana, Cvetić Antić, Tijana, Todorović, Nataša, Živić, Miroslav, Zakrzewska, Joanna, "Selenite metabolism in the mycelium of the fungus Phycomyces blakesleeanus" in Proceedings: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry: Physical Chemistry 2022, Vol. 1; 2022 Sep 26-30; Belgrade, Serbia (2022):93-96,
https://hdl.handle.net/21.15107/rcub_ibiss_5733 .

TY  - JOUR
AU  - Stanić, Marin
AU  - Križak, Strahinja
AU  - Jovanović, Mirna
AU  - Pajić, Tanja
AU  - Ćirić, Ana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antić, Tijana
AU  - Todorović, Nataša
AU  - Živić, Miroslav
PY  - 2017
UR  - http://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000429
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2730
AB  - Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 μM NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33±5 and 21±3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.
T2  - Microbiology
T1  - Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites
IS  - 3
VL  - 163
DO  - 10.1099/mic.0.000429
SP  - 364
EP  - 372
ER  - 

@article{
author = "Stanić, Marin and Križak, Strahinja and Jovanović, Mirna and Pajić, Tanja and Ćirić, Ana and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antić, Tijana and Todorović, Nataša and Živić, Miroslav",
year = "2017",
abstract = "Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 μM NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33±5 and 21±3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.",
journal = "Microbiology",
title = "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites",
number = "3",
volume = "163",
doi = "10.1099/mic.0.000429",
pages = "364-372"
}

Stanić, M., Križak, S., Jovanović, M., Pajić, T., Ćirić, A., Žižić, M., Zakrzewska, J., Cvetić Antić, T., Todorović, N.,& Živić, M.. (2017). Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology, 163(3), 364-372.
https://doi.org/10.1099/mic.0.000429

Stanić M, Križak S, Jovanović M, Pajić T, Ćirić A, Žižić M, Zakrzewska J, Cvetić Antić T, Todorović N, Živić M. Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology. 2017;163(3):364-372.
doi:10.1099/mic.0.000429 .

Stanić, Marin, Križak, Strahinja, Jovanović, Mirna, Pajić, Tanja, Ćirić, Ana, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antić, Tijana, Todorović, Nataša, Živić, Miroslav, "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites" in Microbiology, 163, no. 3 (2017):364-372,
https://doi.org/10.1099/mic.0.000429 . .

TY  - JOUR
AU  - Krizak, Strahinja
AU  - Nikoic, Ljiljana
AU  - Stanic, Marina
AU  - Zizic, Milan
AU  - Zakrzewska, Joanna
AU  - Zivic, Miroslav
AU  - Todorović, Nataša
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1977
AB  - We describe here whole-cell currents of droplets prepared from the
   apical region of growing Phycomyces blakesleeanus sporangiophores.
   Whole-cell current recordings revealed the osmotically activated,
   outwardly rectifying, fast inactivating instantaneous current (ORIC)
   with biophysical properties closely resembling volume-regulated anionic
   current (VRAC). ORIC is activated under conditions of osmotically
   induced swelling and shows strong selectivity for anions over cations.
   In addition, ORIC shows voltage and time-dependent inactivation at
   positive potentials and recovery from inactivation at negative
   potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion
   channel blocker, in a voltage-dependent manner. This is the first report
   of the presence of VRAC-like current in an organism outside the chordate
   lineage. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS.
   All rights reserved.
T2  - Research in Microbiology
T1  - Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores
IS  - 3
VL  - 166
DO  - 10.1016/j.resmic.2015.02.004
SP  - 162
EP  - 173
ER  - 

@article{
author = "Krizak, Strahinja and Nikoic, Ljiljana and Stanic, Marina and Zizic, Milan and Zakrzewska, Joanna and Zivic, Miroslav and Todorović, Nataša",
year = "2015",
abstract = "We describe here whole-cell currents of droplets prepared from the
   apical region of growing Phycomyces blakesleeanus sporangiophores.
   Whole-cell current recordings revealed the osmotically activated,
   outwardly rectifying, fast inactivating instantaneous current (ORIC)
   with biophysical properties closely resembling volume-regulated anionic
   current (VRAC). ORIC is activated under conditions of osmotically
   induced swelling and shows strong selectivity for anions over cations.
   In addition, ORIC shows voltage and time-dependent inactivation at
   positive potentials and recovery from inactivation at negative
   potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion
   channel blocker, in a voltage-dependent manner. This is the first report
   of the presence of VRAC-like current in an organism outside the chordate
   lineage. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS.
   All rights reserved.",
journal = "Research in Microbiology",
title = "Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores",
number = "3",
volume = "166",
doi = "10.1016/j.resmic.2015.02.004",
pages = "162-173"
}

Krizak, S., Nikoic, L., Stanic, M., Zizic, M., Zakrzewska, J., Zivic, M.,& Todorović, N.. (2015). Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores. in Research in Microbiology, 166(3), 162-173.
https://doi.org/10.1016/j.resmic.2015.02.004

Krizak S, Nikoic L, Stanic M, Zizic M, Zakrzewska J, Zivic M, Todorović N. Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores. in Research in Microbiology. 2015;166(3):162-173.
doi:10.1016/j.resmic.2015.02.004 .

Krizak, Strahinja, Nikoic, Ljiljana, Stanic, Marina, Zizic, Milan, Zakrzewska, Joanna, Zivic, Miroslav, Todorović, Nataša, "Osmotic swelling activates a novel anionic current with VRAC-like
 properties in a cytoplasmic droplet membrane from Phycomyces
 blakesleeanus sporangiophores" in Research in Microbiology, 166, no. 3 (2015):162-173,
https://doi.org/10.1016/j.resmic.2015.02.004 . .