@conference{
author = "Rajaković, Mladen and Sofrenić, Ivana and Stojković, Dejan and Glamočlija, Jasmina",
year = "2024",
abstract = "Hericium erinaceus (Bull.) Persoon (fam. Hericiaceae) or Lion’s mane, widely used in traditional Chinese medicine, represents one of the most promising mushrooms with many biological activities, such as neuroprotective, neuroregenerative and antitumor. The main focus of this study was to investigate the antibacterial potential of four extracts obtained from H. erinaceus fruiting bodies using ultrasound-assisted extraction with water, ethanol, ethyl acetate and dichloromethane/methanol 2:1 (v/v) mixture. The 1H NMR profiles revealed the metabolite fingerprint of all four extracts. The highest amount of phenolic compounds, such as caffeic and ferulic acids, was detected in ethyl acetate extract, comparing the total ionic current of the detected compounds between all four extracts obtained by HPLC-Orbitrap-MS/MS. Antibacterial activity of the extracts was evaluated by microdilution method against the following pathogenic bacteria: Pseudomonas aeruginosa, resistant P. aeruginosa, Escherichia coli, Staphylococcus lugdunensis, S. aureus and methicillin-resistant S. aureus (MRSA). Compared to the other two, dichloromethane/methanol and ethyl acetate extracts possessed higher antibacterial activity against P. aeruginosa and resistant P. aeruginosa, with MIC and MBC values of 2 mg/mL and 4 mg/mL for P. aeruginosa and 3 mg/mL and 4 mg/mL for resistant P. aeruginosa. In case of E. coli, ethyl acetate extract had a MIC value of 3 mg/mL, while all extracts had a MBC value of 8 mg/mL. The obtained MIC values of 2 mg/mL for S. lugdunensis and 3 mg/mL for S. aureus and MRSA and MBC value of 4 mg/mL for S. lugdunensis, S. aureus and MRSA indicate that ethyl acetate extract was empirically validated as the most appropriate, among tested extracts, regarding the obtained antibacterial potential, and should be considered for further research. The obtained results emphasize the medicinal potential of H. erinaceus different extracts, especially considering the worldwide trend towards seeking supplements of natural origin.",
publisher = "Belgrade: University of Belgrade, Faculty of Agriculture",
journal = "Book of abstracts: The 3rd International UNIFood Conference: UNIFood2024 Conference; 2024 Jun 28-29; Belgrade, Serbia",
title = "Chemical profiling and antibacterial activity of Hericium erinaceus extracts",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7027"
}
