@conference{
author = "Brdarić, Emilija and Popović, Dušanka and Kulaš, Jelena and Soković Bajić, Svetlana and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Crevna mikrobiota ima važnu ulogu u održavanju homeostaze koju ostvaruje preko interakcija sa imunskim
sistemom i gastrointestinalnim traktom. Toksične supstance ostvaruju direktan štetni efekat na sastav
i funkcionalnost crevne mikrobiote. Kadmijum (Cd) je metal sa izraženom toksičnošću, koji se povezuje
sa brojnim oboljenjima. U našoj studiji je predložen mehanizam zaštite koji bi se ostvario posredstvom
bakterijskih egzopolisaharida (EPS) izolovanih iz soja Lactiplantibacillus plantarum BGAN8, a bazira se
na visokom afinitetu adsorpcije kadmijumovih jona. Ciljevi rada su bili utvrđivanje efekata produženog
delovanja kadmijuma na sastav crevne mikrobiote Dark agouti (DA) pacova, kao i izučavanje efekta
EPS-AN8 na ublažavanje takvih štetnih efekata.
Pacovi su kroz vodu unosili nižu (5 ppm) i višu (50 ppm) dozu kadmijuma, dok je EPS-AN8 (100 μg/ml) dat
kroz hranu za životinje. Totalna DNK je izolovana iz lumena duodenuma i 16S rDNK amplikon je sekvenciran
strategijom uparenih krajeva na Illumina NovaSeq platformi. Dodatno, praćene su promene u
inflamaciji i oksidativnom stresu homogenata duodenuma. Kadmijum je favorizovao rast oportunističkih
patogenih bakterija poput pripadnika rodova Blautia, Prevotella, Alloprevotela, Bacteroides, dok je
relativna zastupljenost laktobacila bila značajno smanjena. Obe doze kadmijuma su stimulisale produkciju
proinflamatornih citokina (IL-1β, TNF-α i IFN-γ) i povisile nivo oksidativnog stresa (MDA, CAT i
GST). Oralni unos EPS-AN8 je značajno ublažio sve navedene promene. Oralna izloženost kadmijumu
uzrokuje nedvosmislene promene u sastavu mikrobiote, koje mogu biti praćene i pojačanim nivoima
inflamacije i oksidativnog stresa. Nasuprot tome, istovremeni unos EPS-AN8 redukuje pojavu takvih
promena., The gut microbiota has important role in maintaining homeostasis via its effect on the immune system
and healthy gastrointestinal tract. Environmental toxicants directly influenced the changes in gut microbiota
composition. Cadmium (Cd) is a hazard, world widely distributed metal. We have proposed
the putative mechanism of protection against Cd harmful effect in the organism by using exopolysaccharides
derived from Lactiplantibacillus plantarum BGAN8 (EPS-AN8) which has great ability to sequester Cd ions.
Aims of our study were to examine Cd related changes in the gut microbiota of Dark agouti (DA) rats after
prolonged oral exposure (30 days) to this metal and to determine impact of EPS-AN8 in preventing
those changes.
Rats were given via water lower (5 ppm) and higher (50 ppm) Cd’s dose. In paralel, EPS-AN8 was administrated
through food (100 μg/ml). Total DNA from duodenum was isolated and PCR amplicon for 16SrRNA was
sequenced on Illumina NovaSeq paired end platform. Moreover, we have followed changes in
oxidative stress and inflammation in duodenum. Cd favored growth of opportunistic pathogen bacteria
genera (Blautia, Prevotella, Alloprevotela, Bacteroides) while the relative abundance of lactobacilli was
decreased. The both doses of Cd increased production of inflammatory cytokines (IL-1β, TNF-α, and
IFN-γ). Also, parameters of oxidative stress were significantly upregulated (MDA, CAT, and GST).
All the mentioned changes were alleviated by EPS-AN8. Oral exposure to Cd lead to inevitable changes
in the microbiota structure followed by inflammation and oxidative stress of surrounding tissue, while
administration of EPS-AN8 reversed those changes.",
publisher = "Belgrade: Serbian Society of Toxicology",
journal = "Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia",
title = "Gut microbiota as a target of toxic compounds, Crevna mikrobiota kao osetljiva meta delovanja toksičnih supstanci",
pages = "92-93",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6113"
}